Journal of Microbiology and Biotechnology

  • 제14권5호
  • /
  • Pages.932-937
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  • 2004
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Improvement of Hydrocarbon Recovery by Two-Stage Cell-Recycle Extraction in the Cultivation of Botryococcus braunii

  • An, Jin-Young (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Kim, Byung-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Lee, Jin-Suk (Biomass Research Team, Korea Institute of Energy Research)
  • 발행 : 2004.10.01
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초록

In situ extraction by organic solvent was studied in order to improve the recovery yield of hydrocarbon from the culture of Botryococcus braunii, a green colonial microalga. When the solvent mixture of octanol as an extractive solvent and n-octane as a biocompatible solvent was added to a two-phase column, the algal growth was seriously inhibited, even at a low concentration of polar octanol. Therefore, a two-stage cell-recycle extraction process was proposed to improve the contact area between the organic phase and the aqueous phase. The hydrocarbon recovery with in situ cell-recycle extraction showed a three-fold increase (57% of cell) in yield over that with two-phase extraction. In addition, over 60% of the hydrocarbon could be recovered without serious cell damage by downstream separation when this process was applied to the culture broth after batch fermentation.

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참고문헌

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