Journal of Microbiology and Biotechnology

  • 제14권1호
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  • Pages.193-196
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  • 2004
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Immobilization of Arg-Gly-Asp (RGD) Sequence in Sugar-Containing Copolymer for Culturing Fibroblast Cells

  • Na, Kun (College of Pharmacy, Chosun University) ;
  • Park, Keun-Hong (Department of Biomedical Engineering, Ajou University)
  • 발행 : 2004.02.01
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초록

The peptide Arg-Gly-Asp (RGD) was immobilized through their amino terminus to ends of a sugar bearing copolymer, producing a functional hybrid copolymer. Poly(N-p-vinylbenzyl-D-maltonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRGDS) [p(VMA-co-VBGRGDS)] promoted the attachment and growth of NIH fibroblast cells. The interaction between fibroblast cells and p(VMA-co- VBGRGDS) copolymer resulted in effective cell attachment, proliferation, and morphological changes by introduction of a GRGDS sequence. Moreover, when pretreated with soluble RGD monomer, attachment of fibroblast cells was suppressed approximately 50% from that of the p(VMA-co-VBGRGDS) surface.

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참고문헌

  1. Barrera, D. A., E. Zylstra, P. T. Lansbury, and R. Langer. 1993. Synthesis and RGD peptide modification of a new biodegradable copolymer system: Poly(lactic acid-colysine). J. Am. Chem. Soc. 115: 11010-11011 https://doi.org/10.1021/ja00076a077
  2. Brandley, B. K. and R. L. Schnaar. 1998. Covalent attachment of an Arg-Gly-Asp sequence peptide to derivatizable polyacrylamide surfaces: Support of fibroblast adhesion and long-term growth. Anal. Biochem. 172: 270-278
  3. Cook, A. D., J. S. Hrkach, N. Cao, I. M. Johnson, U. B. Pajvani, S. M. Cannizaro, and R. Langer. Characterization and development of RGD-peptide-modified poly(lactic acidco- lysine) as an interactive, resorbable biomaterial. J. Biomed. Mater. Res. 35: 513-523
  4. Dai, W., J. Bekt, and W. M. Saltman. 1994. Cell-binding peptides conjugated to poly(ethylene glycol) promote neural cell aggregation. Bio/Technology 12: 797-801
  5. Dai, W. and W. M. Saltzman. 1996. Fibroblast aggregation by suspension with conjugates of poly(ethylene glycol) and RGD. Biotechnol. Bioeng. 50: 349-356
  6. Drumheller, P. D., D. L. Elbert, and J. A. Hubbell. 1994. Multifunctional poly(ethylene glycol) semi-interpenetrating networks as highly selective substrates for bioadhesive peptide grafting. Biotechnol. Bioeng. 43: 772-780
  7. vFalb, R. D. and G. A. Grode. 1971. Covalent bonding of proteins to solid surfaces. Fed. Proc. 30: 1688-1691
  8. Ikada, Y. 1994. Surface modification of polymers for medical applications. Biomaterials 15: 725-736
  9. Kim, S.-K., S.-H. Yu, J.-H. Lee, J.-Y. Lee, A. Rademacher, D.-H. Lee, and J.-K. Park. 2001. Effect of collagen concentration on the viability and metabolic function of encapsulated hepatocytes. J. Microbiol. Biotechnol. 11: 418-421
  10. Kobayashi, K. and H. Sumitomo. 1988. Oligosaccharidecarrying styrene-type macromers. Polymerization and specific interactions between the polymers and liver cells. J. Macromol. Sci. Chem. A25: 655-667
  11. Lee, D.-H., J.-H. Lee, J.-E. Choi, Y.-J. Kim, S.-K. Kim and J.-K. Park. 2002. Determination of optimum aggregates of porcine hepatocytes as a cell source of a bioartificial liver. J. Microbiol. Biotechnol. 12: 735-739
  12. Lee, J. H., J. W. Jung, I.-K. Kang, and H. B. Lee. 1994. Cell behavior on polymer surfaces with different functional groups. Biomaterials 15: 705-711
  13. Lin, H. B., Z. C. Zhao, C. Garcia-Exheverria, D. H. Rich, and S. L. Cooper. 1991. Synthesis of novel polyurethane copolymer containing covalently attached RGD peptide. J. Biomater. Sci. Polym. Eds. 3: 217-227
  14. Maeda, M., Y. Izuno, K. Kawasaki, Y. Kaneda, Y. Mu, Y. Yasuo, S. Nakagawa, and T. Mayumi. 1997. Amino acids and peptides. Preparation of Arg-Gly-Asp (RGD) hybrids with poly(ethylene glycol) analogs and their antimetastatic effects. Chem. Pharm. Bull. 45: 1788-1792
  15. Massia, S. P. and J. A. Hubbell. 1990. Covalent surface immobilization of Arg-Gly-Asp- and Try-Ile-Gly-Ser-Argcontaining peptides to obtain well-defined cell adhesive substrates. Anal. Biochem. 187: 292-301
  16. Massia, S. P. and J. A. Hubbell. 1992. Vascular endothelial cell adhesion and spreading promoted by the peptide REDV of the IICS region of plasma fibronectin is mediated by integrin $\alpha$4b1. J. Biol. Chem. 267: 14019-14026
  17. Na, K., H.-K. Choi, T. Akaike, and K.-H. Park. 2001. Conjugation of Arg-Gly-Asp (RGD) sequence in copolymer bearing sugar moiety for insulinoma cell (MIN6) culture. Biosci. Biotech. Biochem. 65: 1284-1289
  18. Na, K., D.-W. Kim, and K.-H. Park. 2001. Certification of fibroblast cell adhesion and spreading mediated by Arg-Gly- Asp (RGD) sequence on thermo-reversible hydrogel. J. Microbiol. Biotechnol. 11: 922-927