Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning, Purification, and Characterization of an Extracellular Nuclease from Aeromonas hydrophila ATCC14715

  • Nam, In-Young (Department of Bioscience and Biotechnology, Hankuk University) ;
  • Myung, Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University) ;
  • Joh, Ki-Seong (Department of Bioscience and Biotechnology, Hankuk University)
  • Published : 2004.02.01
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Abstract

A gene encoding an extracellular nuclease was cloned from Aeromonas hydrophila strain ATCC14715. The gene was overexpressed and the enzyme was purified by fusing to maltose binding protein. It was shown that the protein possessed DNase activity on both single-stranded and double-stranded DNAs. It exhibited both endo- and exonuclease activities. It was also shown that the protein had an RNase activity. Possible roles of this extracellular enzyme in the A. hydrophila life cycle are discussed.

Keywords

References

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