Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Pathogenicity and Single Dose Toxicity of a Potential Probiotic Lactobacillus spp. PSC101 in Mice

  • Hwang, Mi-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Young-Hwon (Kyungpook Veterinary Service Laboratory) ;
  • Kim, Eun-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Song, Jae-Chan (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Keun-Woo (College of Veterinary Medicine, Kyungpook National University) ;
  • Jeong, Kyu-Shik (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man-hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Oh-Deok (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2004.06.01
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Abstract

This study was conducted to investigate the pathogenicity and acute single toxicity of Lactobacillus spp. PSC101 (PSC101) isolated from pigs and L. acidophilus (LA) at 2.5$\times$$10^9$CFU or 2.5$\times$$10^{12}$colony forming units (CFU) in mice for 14 days. After oral administration of the bacteria into mice, we could not find their any specific pathogenicity from the standpoints of clinical signs, and changes in body weight and body temperature, as compared with the control group during 14 days. We further investigated the toxicity of concentrated culture broth ($\times$10) after fermentation of them for safe industrial process. As the results, we could not find any clinical signs, changes in body weight and body temperature, as compared with the control group (MRS broth) for 14 days. The results obtained in this study suggest that the potentially probiotic, PSC101, is non-toxic in mice and is therefore likely to be safe for pig use.

Keywords

References

  1. Abe, F., Ishibashi, N. and Shimamura, S. (1995): Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets. J. Dairy. Sci., 78, 2838-2846
  2. Adami, A. and Cavazzoni, V. (1999): Occurrence of selected bacterial groups in the faeces of piglets fed with Bacillus coagulans as probiotic. J. Basic Microbiol., 39, 3-9 https://doi.org/10.1002/(SICI)1521-4028(199903)39:1<3::AID-JOBM3>3.0.CO;2-O
  3. Chou, L.S. and Weimer, B. (1999): Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J. Dairy. Sci., 82, 23-31
  4. Ishibashi, N. and Yamazaki, S. (2001): Probiotics and safety. Am. J. Glin. Nutr., 73, 465S-470S
  5. Kaur, J.P., Chopra, K. and Saini, A. (2002): Probiotics: potential pharmaceutical applications. Eur. J. Pharm. Sci., 15, 1-9
  6. Reid, G. (2002): The role of cranberry and probiotics in intestinal and urogenital tract health. Grit. Rev. Food Sci. Nutr., 42, 293-300
  7. Reid, G. and Friendship, R. (2002): Alternatives to antibiotic use: probiotics for the gut. Anim. Biotechnol., 13, 97-112
  8. Saarela, M., Mogensen, G., Fonden, R., Malto, J. and Mattila- Sandholm, T. (2000): Probiotic bacteria: safety, functional and technological properties. J. Biotechnol., 84, 197-215 https://doi.org/10.1016/S0168-1656(00)00375-8
  9. Vanbelle, M., Teller, E. and Focant, M. (1990): Probiotics in animal nutrition: a review. Arch. Tierernahr., 40, 543-567
  10. Verstegen, M.W. and Williams, B.A. (2002): Alternatives to the use of antibiotics as growth promoters for monogastric animals: Anim. Biotechnol., 13, 113-127
  11. Zhou, J.S., Shu, Q., Rutherfurd, K.J., Prasad, J., Gopal, P.K. and Gill, H.S. (2000): Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Ghem. Toxicol., 38, 153-161