Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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The mRNA Expression of Radio-Sensitive Genes Exposed to Various Dosage of Ionizing Radiation in U-937 Cell

U-937 세포에서 이온화 방사선의 조사선량에 따른 감수성 유전자들의 발현 변화

  • 김종수 (경기대학교 자연과학부 생물학과) ;
  • 임희영 (경기대학교 자연과학부 생물학과) ;
  • 오연경 (경기대학교 자연과학부 생물학과) ;
  • 김인규 (한국원자력연구소 방사선 생체 해석분야) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실) ;
  • 윤병수 (경기대학교 자연과학부 생물학과)
  • Published : 2004.03.01
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Abstract

We used cDNA microarray to assess gene expression profiles in hematopoetic cell line, U-937, exposed to low doses of ionizing irradiation. The 1,000 DNA elements on this array were PCR-amplified cDNAs selected from named human cancer related genes. According to the strength of irradiation, the levels of some gene expression were increased or decreased as dose-dependent manner. The gene expressions of Tubulin alpha, protein kinase, interferon-alpha, -beta, -omega receptor and ras homolog gene family H were significantly increased. Especially, Tubulin gene was shown 2.5 fold up-regulated manner under stress of 500 rad irradiation than 200 rad. On the other hand, fibroblast growth factor 12 and four and a half LIM domains, etc. were significantly down-regu-lated. Also, tumor protein 53(TP53) related genes that p53 inducible protein, tumor protein 53-binding protein looks of little significance as radiation sensitive manner. The radio-sensitivity of tubulin gene etc. that we proposed could be useful to rapid and correct survey for the bio-damage by exposure to low dose irradiation.

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References

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