Restorative Dentistry and Endodontics

  • 제29권4호
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  • Pages.399-408
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  • 2004
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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상아모세포 관련 유전자, OD314의 발현과 기능 연구

EXPRESSION AND FUNCTIONAL CHARACTERIZATION OF ODONTOBLAST-DERIVED GENE: OD314

  • 김두현 (조선대학교 치과대학 구강조직학교실) ;
  • 김흥중 (조선대학교 치과대학 구강조직학교실) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실) ;
  • 손호현 (서울대학교 치과대학 치과보존학교실) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실)
  • Kim, Doo-Hyun (Department of Oral Histology, School of Dentistry, Chosun University) ;
  • Kim, Heung-Joong (Department of Oral Histology, School of Dentistry, Chosun University) ;
  • Jeong, Moon-Jin (Department of Oral Histology, School of Dentistry, Chosun University) ;
  • Son, Ho-Hyun (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Park, Joo-Cheol (Department of Oral Histology, School of Dentistry, Chosun University)
  • 발행 : 2004.07.01
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초록

Odontoblasts are responsible for the formation and maintenance of dentin. They are known to synthesize unique gene products including dentin sialophosphoprotein (DSPP). Another unique genes of the cells remain unclear. OD314 was isolated from the odontoblasts/pulp cells of rats and partially characterized as an odontoblast-enriched gene (Dey et al., 2001). This study aimed to elucidate the biological function of OD314, relating to odontoblast differentiation and dentinogenesis. After determining the open reading frame (ORP) of OD314 by transient transfection analysis using green fluorescent protein (GPP) expression vector, mRNA in-situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and western analysis were performed. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were expressed in odontoblasts of developing coronal and root pulp. 2. OD314 was a novel protein encoding 154 amino acids, and the protein was mainly expressed in cytoplasm by transient transfection analysis. 3. Mineralized nodules were associated with multilayer cell nodules in the culture of human dental pulp cells and first detected from day 21 using alizarin-red S staining. 4. In RT-PCR analysis, OD314, osteocalcin (OC) and DSPP strongly expressed throughout 28 days of culture. Whereas, osteonectin (ON) mRNA expression stayed low up to day 14, and then gradually decreased from day 21. 5. Western blots showed an approximately 17 kDa band. OD314 protein was expressed from the start of culture and then increased greatly from day 21. In conclusion, OD314 is considered as an odontoblast-enriched gene and may play important roles in odontoblast differentiation and dentin mineralization.

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