Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Comparison of DNase Activities from Infective Larvae vs Adult Worms of Haemonchus contortus

염전위충 감염자충과 성충의 DNase 활성 비교

  • 곽동미 (워싱턴주립대학교 수의과대학)
  • Published : 2004.09.01
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Abstract

DNase activity from infective larvae of the parasitic nematode Haemonchus contortus was characterized and compared to that from whole worm. DNase activity from infective larvae was detected throughout pHs 4-10, but high activity was detected under acidic conditions. The activity was not inhibited by 10 mM EDTA at pH 5.0, but was significantly inhibited at pH 7.0. The activity produced DNA, fragments with mixtures of 3'-hydroxyls (OH) and 3'-phosphates (P) at each pH with predominance of 3'-P. A unique DNase activity at 37 kDa was identified from infective larvae on zymograms. The 37 kDa DNase was detected only at pH 5.0, but not at pH 7.0, and this activity was not inhibited by EDTA at pH 5.0. These characteristics of the 37 kDa infective larval DNase resemble those of classic acidic DNases (e.g., DNase II). In contrast, 34, 36 and 38.5 kDa DNase activities were shown to be specific for whole worm. This result demonstrated that DNases in H contortus are regulated during development.

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References

  1. Gasmi G, Pasdeloup M, Pratviel G, Pitie M, Bernadou J, Meunier B. 31 P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/ KHSO5' in comparison with DNases I and II. Nucleic Acids Res 1991; 19: 2835-2839 https://doi.org/10.1093/nar/19.11.2835
  2. Hevelone J, Hartman PS. An endonuclease from Caenorhabditis elegans: partial purification and characterization. Biochem Genet 1988; 26: 447-461 https://doi.org/10.1007/BF02399412
  3. Jasmer DP, Wescott RB, Crane JW. Survival of third-stage larvae of Washington isolates of Haemonchus contortus and Ostertagia circumcincta exposed to cold temperatures. Proc Helminthol Soc Wash 1987; 54: 48-52
  4. Kwak D. Characterization of DNase activity from nymph stage midgut of Boophilus microplus tick. Kor J Vet Publ Hlth 2004; 28: 51-58
  5. Kwak D, Jasmer DP. Non-classic characteristics define prominent DNase activities from the intestine and other tissues of Haemonchus contortus. Exp Parasitol 2003; 104: 131-139 https://doi.org/10.1016/S0014-4894(03)00136-X
  6. Liu MY, Cho SM, Tsai WS, Chang MH, Chen JY, Yang CS. Prevalence of antibody to Epstein-Barr virus DDDNase in children. Intervirology 1992; 34: 192-196
  7. Mak CH, Ko RC. Characterization of endonuclease activity from excretory/secretory products of a parasitic nematode, Trichinella spiralis. Eur J Biochem 1999; 260: 477-481
  8. McKeand JB. Vaccine development and diagnostics of Dictyocaulus viviparus. Parasitology 2000; 120: S17-S23 https://doi.org/10.1017/S0031182099005727
  9. Mortensen LL, Williamson LH, Terrill TH, Kircher RA, Larsen M, Kaplan RM. Evaluation of prevalence and clinical implications of anthelmintic resistance in gastrointestinal nematodes in goats. J Am Vet Med Assoc 2003; 223: 495-500 https://doi.org/10.2460/javma.2003.223.495
  10. Redmond DL, Knox DP, Newlands G, Smith WD. Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus. Mol Biochem Parasitol 1997: 85: 77-87 https://doi.org/10.1016/S0166-6851(96)02812-5
  11. Shiokawa D, Tanuma S. Characterization of human DNase I family endonucleases and activation of DNase gamma during apoptosis. Biochemistry 2001; 40: 143-152 https://doi.org/10.1021/bi001041a
  12. Skuce PJ, Redmond DL, Liddell S, Stewart EM, Newlands GF, Smith WD, Knox DP. Molecular cloning and characterization of gut-derived cysteine proteinases associated with a host protective extract from Haemonchus contortus. Parasitology 1999; 119: 405-412 https://doi.org/10.1017/S0031182099004813
  13. Smith SK, Pettit D, Newlands GF, Redmond DL, Skuce PJ, Knox DP, Smith WD. Further immunization and biochemical studies with a protective antigen complex from the microvillar membrane of the intestine of Haemonchus contortus. Parasite Immunol 1999; 21: 187-199 https://doi.org/10.1046/j.1365-3024.1999.00217.x
  14. Smith WD, Newlands GF, Smith SK, Pettit D, Skuce PJ. Metalloendopeptidases from the intestinal brush border of Haemonchus contortus as protective antigens for sheep. Parasite Immunol 2003; 25: 313-323 https://doi.org/10.1046/j.1365-3024.2003.00637.x
  15. Smith WD, Smith SK, Murray JM. Protection studies with integral membrane fractions of Haemonchus contortus. Parasite Immunol 1994; 16: 231-241 https://doi.org/10.1111/j.1365-3024.1994.tb00345.x