Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Alterations of c-Fos mRNA Expression in Hypothalamic-Pituitary-Adrenal Axis and Various Brain Regions Induced by Intrathecal Single and Repeated Substance P Administrations in Mice

  • Choi, Seong-Soo (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Lee, Han-Kyu (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Shim, Eon-Jeong (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Kwon, Min-Soo (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Seo, Young-Jun (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Lee, Jin-Young (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Suh, Hong-Won (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University)
  • Published : 2004.08.01
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Abstract

The effect of substance P (Sub P) injected intrathecally (I.t.) on c-fos mRNA expression in vari-ous tissues was examined in the present study. We found that a single administration of Sub P(0.5 nM) caused an increase of the c-fos mRNA level in the hypothalamic-pituitary-adrenal(HPA) axis, hippocampus, and spinal cord. The time-course study showed that c-fos mRNA level was maximal at 10 min and began to decrease 30 min after the Sub P injection in all tis-sues, and the Sub P-induced increase of the c-fos mRNA level was returned to the control level 1 h after the injection. The kinetics of the c-fos mRNA expression in mice that were repeatedly injected with Sub P (every 30 min interval up to 4 times) were different in the HPA axis, hippocampus, and spinal cord. The increased c-fos mRNA level in the hypothalamus and the spinal cord induced by I.t. injected Sub P remained at a high level. In the pituitary gland, adrenal gland, and hippocampus, the increased level of c-fos mRNA expression gradually returned to the control level during the repeated substance P injections up to 4 times. Our results suggest that spinally injected Sub P-induced pain stress increases c-fos mRNA expres-sion in the spinal cord, hippocampus, and HPA axis. In mice repeatedly injected with Sub P, the kinetics of c-fos mRNA appear to be different varied from tissue to tissue.

Keywords

References

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