Biotechnology and Bioprocess Engineering:BBE

  • 제9권4호
  • /
  • Pages.256-260
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  • 2004
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Decolorization of Melanin by Lignin Peroxidase from Phanerochaete chrysosporium

  • Woo, Sung-Hwan (Department of Biological Engineering, Inha University) ;
  • Cho, Jeung-Suk (Department of Biological Engineering, Inha University) ;
  • Lee, Baek-Seok (Department of Biological Engineering, Inha University) ;
  • Kim, Eun-Ki (Department of Biological Engineering, Inha University)
  • 발행 : 2004.07.01
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초록

Melanin was decolorized by lignin peroxidase from Phanerochaete chrysosporium. This decolorization reaction showed a Michaelis-Mentens type relationship between the decolorization rate and concentration of two substrates: melanin and hydrogen peroxide. Kinetic constants of the decolorization reaction were 0.1 OD$\sub$475//min ($V_{max}$) and 99.7 mg/L ($K_{m}$) for melanin and 0.08 OD$\sub$475//min ($V_{max}$) and 504.9 ${\mu}$M ($K_{m}$) for hydrogen peroxide, respectively. Depletion of hydrogen peroxide interrupted the decolorization reaction, indicating the essential requirement of hydrogen peroxide. Pulsewise feeding of hydrogen peroxide continued the decolorizing reaction catalyzed by lignin peroxidase. These results indicate that enzymatic decolorization of melanin has applications in the development of new cosmetic whitening agents.

키워드

참고문헌

  1. Melanins and Melanogenesis Pigment cell metabolism: Enzymatic and chemical control Prota, G.
  2. Chem. Res. Toxicol. v.11 Photoprotective actions of natural and synthetic melanins Krol, E. S.;D. C. Liebler https://doi.org/10.1021/tx980114c
  3. J. Soc. Cos. Sci. Korea v.26 The 21C research trend on cosmeceuticals Horii, I.
  4. J. Microbiol. Biotechnol. v.12 Purification and Characterization of 2,4-Dichloropheneol Oxidizing Peroxidase from Streptomyces sp. AD001 Jeon, J. H.
  5. Biotechnol. Bioprocess Eng. v.8 The selective visualization of lignin peroxidase, manganese peroxidase and laccase, produced by white rot fungi on solid media Ryu, W. T.;M. Y. lang;M. H. Cho https://doi.org/10.1007/BF02940268
  6. Enzyme Microb. Technol. v.28 Growth, dye degradation, and ligninolytic activity studies on Zimbabwean white-rot fungi Tekere, M.;A. Y. Mswaka;R. Zvavya;J. S. Read https://doi.org/10.1016/S0141-0229(00)00343-4
  7. Biotechnol. Bioprocess Eng. v.8 Biodegradation of endocrine-disrupting phenolic compounds using laccase followed by activated sludge treatment Nakamura, Y.;G. Mtui https://doi.org/10.1007/BF02949220
  8. Appl. Microbiol. Biotechnol. v.42 Decolourisation and depolymerisaton of solubilised low lank coal by the white rot basidiomycete Phanerochaete chrysosporium Ralph, J. P.;D. E. A. Catcheside https://doi.org/10.1007/BF00173917
  9. Method. Enzymol. v.161 Lignin peroxidase of Phanerochaete chrysosporium Tien, M.;T. K. Kirk https://doi.org/10.1016/0076-6879(88)61025-1
  10. Appl. Microbiol. Biotechnol. v.46 Depolymerization of low-rank coal by extracellular fungal enzyme systems: I. Screening for low-rank-coal-depolymerizing activities Hofrichter, M.;W. Fritsche https://doi.org/10.1007/s002530050808
  11. Arch. Microbiol. v.27 Uber die Einwirkung von Huminsauren auf das Wachstum einiger Penicillium-marten Flaig, W.;H. L. Schmidt
  12. Aust. J. Soil Res. v.7 Inhibition and stimulation of proteolytic enzyme activities by soil humic acids Ladd, J. N.;J. H. A. Butler https://doi.org/10.1071/SR9690253
  13. Acta Fac. Rerum. Microbiol. v.8 Decomposition of humic acids from peat soil by micromycetes Macor, M.
  14. Appl. Biochem. Biotechnol. v.20 Depolymerization of water soluble coal polymer from subbitumious coal and lignite by lignin peroxidase Wondrack, L.;M. Szanto;W. A. Wood https://doi.org/10.1007/BF02936524
  15. Appl. Environ. Microbiol. v.59 Decolorizaton of azo, triphenyl methane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes from Phanerochaete chrysosporium Ollikka, P.;K. Alhonmaki;V. M. Leppanen;T. Glumoff;T. Raijola;I. Suominen
  16. J. Microbiol. Biotechnol. v.8 Degradation of polycyclic aromatic hydrocarbons by selected white-rot fungi and the influence of lignin peroxidase Kim, M. S.;E. J. Huh;H. J. Kim;K. W. Mon
  17. Enzyme Microb. Technol. v.24 The evaluation of white rot fungi in the decolorization of textile dyes Swamy, J.;J. A. Ramsay https://doi.org/10.1016/S0141-0229(98)00105-7
  18. Res. Microbiol. v.147 Study of a melanic pigment of Proteus mirabilis Agodi, A.;S. Stefani;C. Corsaro;F. Campanile;S. Gribaldo;S. Sichel https://doi.org/10.1016/0923-2508(96)80216-6