Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Chararterization of Causing Viruses from Acute Conjunctivitis Patients During Year 2001 to 2003.

2001∼2003년 유행성 눈병환자로부터 원인바이러스의 분리 및 특성

  • Published : 2004.08.01
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Abstract

Viruses causing acute conjuntivitis were isolated from 675 patients carrying eye infections for year 2001 to 2003 in Busan reagion and their antigenic properties characterized by a serological survey. In 2001, adenoviruses (serotype 8) were found in 5 of 48 cases. In 2002, the isolated viruses were 7 adenoviruses (serotype 8 and 37), 8 coxsakieviruses (serotype A24 and B3) and 1 echoviruses (serotype 6) from 324 specimens that are known as the causative agents of acute hemorrhagic conjuctivitis (AHC). In 2003, 25 case of 303 specimens were 7 adenoviruses (serotype 3, 4, 8 and 37), 7 echoviruses (serotype 6 and 7) and 4 untypable enteroviruses. Although coxsakievirus (serotype B3) and echoviruses (serotype 6 and 7) were generally known as causative agent of aseptic meningitis, it hasn't been reported until now that they were isolated from the conjunctival swabs. The out break of AC was observed from April to October in Busan. These isolated viruses showed a strong cytophatic effects on HEp-2, RD, Vero and BGM cell strains. Analysis of electron micrograph of those viruses showed that adenovirus consists of a 80 nm diameter and nonenvloped icosahedron and then echovirus and coxsackievirus were small nonenveloped and isometric-shaped viruses. Adenovirus showing a cytophatic effect was resulted in a 458 bp single band by PCR and echovirus, coxsackievirus and untypable enterovirus were detected a 437 bp products by RT-PCR.

2001년부터 2003년까지 3년간 부산지역의 안과 병ㆍ의원으로부터 채취한 급성 바이러스성 결막염 환자의 가검물 675 건을 대상으로 유행한 눈병 원인 바이러스의 분리 결과, 유행성각결막염의 원인바이러스인 Adenovirus 8ㆍ37형, 급성 출혈성결막염(아폴로눈병)의 원인바이러스인 Coxsackievirus A24ㆍB3형, Echovirus 6ㆍ7형을 분리하였고 인두결막열을 일으키는 Adenovirus 3ㆍ4형을 분리하였으며 Coxsackievirus A24는 2002년 아폴로눈병의 대유행과 관련하여 2002 년 8월 국내 최초로 분리하였다. 2002년과 2003년에 분리된 Coxsackievirus B3와 Echovirus 6은 지금까지 뇌수막염을 유발시키는 원인바이러스로 널리 알려져 있으나, 눈병환자에서의 분리된 것은 특징적이었다. 월별 발생양상은 주로 하절기에 집중적으로 분포하였고, 2003년에는 4월과 10월에도 높은 발생율을 보였다. 연령별 발생분포도는 10대에서 50대까지 높은 발생율을 나타내었고 유아와 60대 이상에서도 발생하였고 남녀 발생비는 1.2ㆍ1이었다.

Keywords

References

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