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유해성 적조생물에 대한 Ceratium 추출물 영향 평가

The Influences of Extracts from Ceratium spp, on the Growth of Harmful Microalgae

  • 발행 : 2004.08.01

초록

Ceratium 적조생물을 메타놀이나 수용성 물질을 분리하거나 cell-free medium 추출물을 Cochlodinium polykrikoides 성장에 미치는 영향을 조사했다. cell-free medium을 25% 이상 첨가한 시험구에서 가장 우수한 C. polykrikoides 성장을 가져왔다. 그러나 메타놀이나 수용성으로 처리한 추출물에서는 고농도로 배지에 첨가시켜도 C. polykrikoides 성장에는 큰 영향이 없었다. 특히 cell-free medium은 Gyredinium impudicum이나 Chaetoceros 종에서도 C. polykrikoides와 비슷한 양상을 보였으나, Alxendrium 적조생물에 대해서는 현저히 성장을 저해시키는 결과를 나타내었고, Prorocentrium minimum에서도 성장을 촉진시키지는 못했다. 이러한 결과로 보와서 Ceratium 추출물은 C. polykrikoides성장을 촉진시킬 수 있는 주요한 역할을 하는 것으로 보여서 두 적조생물이 공존하게 되면 상호간에 영향을 미칠 수 있을 것으로 판단된다.

The growth response of the fish-killing dinoflagellate Cochlodinium polykrikoides was studied in cultures, using the treatment of Ceratium extracts by a methanol, a water-soluble, and a cell-free medium. The cell-free medium had the most increasing on the growth of C. polykrikoides cultures, enriched with $\geq$ 25% Ceratium, whereas the methanol and water-souble fractions did not affect the growth of C. polykrikoides exposed to even higher concentration. In particular, the cell-free medium also increased the growth of Gyrodinium impudicum and Chaetoceros sp., similar species to C. polykrikoides. In contrast to C. polykrikoides, G. impudicum and Chaetoceros sp., the growth of Alexandrium tamarense was inhibited significantly, and there was no great effect on the growth of Prorocentrum minimum. These results imply that Ceratium extracts may play an important role in the stimulatory effect of C. polykrikoides, and they have to affect the interaction between C. polykrikoides and Ceratium when co-existing.

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참고문헌

  1. Blanchemain, A., D. Grizeau and J. C. Guary. 1994. Effect of different organic buffers on the growth of Skeletonema costatum cultures; further evidence fro an autoinhibiotry effect. J. Plank. Res. 16, 1433-1440 https://doi.org/10.1093/plankt/16.10.1433
  2. Guillard, R. L. and J. H. Ryther. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detommule conferracea (Cleve). Gram. Can. J. Microbiol. 8, 229-239 https://doi.org/10.1139/m62-029
  3. Imada, N., K. Kobayashi, K. Isomura, H. Saito, S. Kimura, K. Tahara and Y. Oshima. 1992. Isolation and identification of an autoinhibitor produced by Skeletonema costatum. Nippon Suisan. Gakkaishi 58, 1687-1692 https://doi.org/10.2331/suisan.58.1687
  4. Imada, N., K. Kobayashi, K. Tahara and Y. Oshima. 1991. Production of an autoinhibitor by Skeletonema costatum and its effect on the growth of other phytoplankton. Nippon Suisan Gakkaishi 57, 2285-2290 https://doi.org/10.2331/suisan.57.2285
  5. Imai, I., Y. Ishida, S. Sawayama and Y. Hata. 1991. Isolation of a marine gliding bacterium that kills Chattonella antiqua (Raphidophyceae). Nippon Suisan Gakkaishi 57, 1409
  6. Kim, H. G., S. G. Lee, K. H. An, S. H. Youn, P. Y. Lee, C. K. Lee, E. S., Cho, J. B. Kim, H. G. Choi and P. J. Kim. 1997. Recent red tides in Korean coastal waters. Kudeok Publishing, Pusan, 292pp (in Korean)
  7. Kondo, K., Y. Seike and Y. Date. 1990. Red tides in the brackish lake Nakanoumi (II): Relationships between the occurrence of Prorocentrum minimum red tide and environmental conditions. Bull. Plank. Soc. Japan 37, 19-34
  8. Lim, W. A., H. G. Kim, W. J. Lee and S. S. Lee. 1993. Composition of fatty acid and the effect of environmental factors on the population growth of Scrippsiella trochoidea a dinoflagellate responsible for a red tide. Bull. Korean Fish. Soc. 26, 198-203 (in Korean)
  9. Mountfort, D.O., M. Atkinsoh, K. Ponikla, B. Burke and K. Todd. 1996. Lysis of Gymnodinium species by the fungus Verticillium lecanii. Bot. Mar. 39, 159-165
  10. Nagasaki, K., M. Ando, I. Imai, S. Itakura and Y. Ishidaq. 1994. Virus-like particles in Heterosigma akashiwo (Raphidophyceae): a possible red tide disintegration mechanism. Mar. Biol. 119, 307-312 https://doi.org/10.1007/BF00349570
  11. Uye, S. I. and K. Tamatsu. 1990. Feeding interactions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser. 59, 97-107 https://doi.org/10.3354/meps059097
  12. Uye, S. I. 1996. Induction of reproductive failure in the planktonic copepod Calanus pacificus by diatoms. Mar. Ecol. Prog. Ser. 133, 89-97 https://doi.org/10.3354/meps133089
  13. Yokote, M., T. Honjo and M. Asakawa. 1985. Histochemical demonstration of a glycocalyx on the cell surface of Heterosigma akashiwo. Mar. Biol. 88, 295-299 https://doi.org/10.1007/BF00392590