Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on the Mycelial Growth of Agrocybe aegerita in Flask Culture.

플라스크배양에서 버들송이버섯 균사체 배양에 관한 연구

  • 차월석 (조선대학교 화학공학과) ;
  • 이명렬 (조선대학교 식품의약학) ;
  • 조배식 (조선대학교 식품의약학과, 광주광역시 보건환경연구) ;
  • 박세영 (조선대학교 식품영양학) ;
  • 오동규 (조선대학교 화학공학과)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Agrocybe aegerita is Hymenomycetes fungus belonging to the order Agaricales and family of Bolbitiaceae. Much less is known about liquid culture of Agrocybe aegerita. Thus, the present study was to investigate the liquid cultural characteristics of Agrocybe aegerita my-celium. The optimal medium for the mycelial growth and density was ME medium, optimal tem-perature and initial pH were 25$\pm$1$^{\circ}C$ and 5.5, respectively. And optimal culture time for mycelial growth was 12 days. The modified optimal medium compositions were dextrin 3% (w/v), yeast extract 2% (w/w), MgSO$_4$0.05% (w/v), and KH$_2$PO$_4$0.15% (w/v). Under optimal culture conditions, the mycelial growth of modified optimal medium was higher than that of ME medium.

버들송이버섯의 균사체 대량 생산의 기초자료를 얻을 일환으로 균사의 영양생장에 필요한 적합한 조건을 연구하고자 균사생장을 위한 최적 배양 조건 및 영양원을 조사한 결과는 다음과 같다 5종의 공시배지 중 ME 배지에서 버들송이 버섯의 균사생장 및 밀도가 가장 양호하였으며, 균사생장최적 온도는 $25^{\circ}C$이고, 최적 pH는 5.5이며 최적 배양일수는 12일이었다. 버들송이 버섯의 균사생장을 위한 최적 배지조성은 탄소원에서는 다당류인 dextrin이었으며, 최적 탄소원인 dextrin의 적정농도는 3% (w/v)였다. 질소원에서는 유기태 질소원인 yeast extract이었고, yeast extract 최적농도는 2% (w/v)였으며, 최적 무기염류는 MgSO$_4$와 KH$_2$PO$_4$이고, 최적 농도는 MgSO$_4$0.05% (w/v), KH$_2$PO$_4$0.15 (w/v)를 혼합하였을 때 가장 많은 생산량을 보였다. 기본배지인 ME 배지와 본 연구에서 얻어진 최적배지로 액체 배양하여 균체량을 비교한 결과 기본배지는 배양 12일째 최대 건조 균체량 7.59 g/l을 얻었으며, 최적배지는 배양 12일째 17.6 g/l의 최대 건조 균체량을 얻어 기본배지 보다 최적배지의 균체량이 훨씬 높은 균사체 생산수율을 보였다.

Keywords

References

  1. Chang, H. Y., D. Y. Cha, A. S. Kang, I. P. Hong, K. P. Kim, S. J. Seok, Y. J. Ryu and J. M. Sung. 1995. Cultural characteristics of Fomitella fraxinea (Fr.) Imaz. Kor. J. Mycol. 23, 238-245
  2. Chi, J. H., T. M. Ha, Y. H. Kim and Y. D. Rho. 1996. Studies on the main factors affecting the mycelial growth of Phellinus linteus. Kor. J. Mycol. 24, 214-222
  3. Chihara, G., J. Hamuro, Y. Y. Maeda, Y. Arai and F. Fukuoka. 1970. Fractionation and purification of the polysaccharide with marked antitumor activity, especially lentinan from Lentinus edodes. Cancer Res. 30, 2776-2781
  4. Go, S. J., C. H. Yoo and D. Y. Cha. 1981. Studies on the artificial substrate with rice straw and the spawning for the Pleurotus florida in Korea. Kor. J. Mycol. 9, 67-72
  5. Ha, H. C., S. Park, C. W. Lee, I. C. Jung, S. H. Kim, Y. I. Kwon and J. S. Lee. 1995. Isolation and purification of protein-bond polysaccharides from the sawdust of Agrocybe cylindracea. Kor. J. Mycol. 23, 121-128
  6. Hashimoto, K. and Z. Takahashi. 1974. Studies on the growth of Pleurotus ostreatus. Mush. Sci. IX, 585-593
  7. Hayakawa, T., H. Yosimura, M. Kanetake and N. Sato. 1991. Chemical composition of yanagimatsutake (Agrocybe cylindracea) cultivated on sawdust medium. Nippon Nogeikagaku Kaishi 65, 747-754 https://doi.org/10.1271/nogeikagaku1924.65.747
  8. Hong, I. P. and M. W. Lee. 1990. Studies on the cultural characteristics of Poria cocos. Kor. J. Mycol. 18, 42-49
  9. Hong, J. S. and K. H. Kang. 1983. Fruit-body formation of Pleurotus florida on the synthetic medium. Kor. J. Mycol. 11, 121-128
  10. Hong, J. S., J. Y. Lee, M. S. Kim and D. H. Kim. 1986. Studies on the production of mycelium by Lyophyllum decastes in submerged culture. Kor. J. Mycol. 14, 131-139
  11. Hong, J. S., Y. J. Kwon and G. T. Jung. 1983. Studies on Basidiomycetes (2) -Production of mushroom mycelium (Pleurotus ostreatus and Auricularia auricula-judae) in shaking culture. Kor. J. Mycol. 11, 1-7
  12. Hong, J. S., Y. H. Choi and S. E. Yun. 1996. Studies on the celluloytic enzymes produced by Ganoderma lucidum in synthetic media. Kor. J. Mycol. 14, 121-130
  13. Jung, I. C., S. H. Kim, Y. I. Kwon, S. Y. Kim, J. S. Lee, S. Park, K. S. Park and J. S. Lee. 1997. Cultural condition for the mycelial growth of Phellinus igniarius on chemically defined medium and grains. Kor. J. Mycol. 25, 133-142
  14. Kang, A. S., D. Y. Cha, I. P. Hong, H. Y. Chang and S. H. Yu. 1994. Studies of cultural condition on the mycelial vegetative growth in Naematoloma sublateritium (Fr.) Karst. Kor. J. Mycol. 22, 153-159
  15. Kang, M. S., T. S. Kang, A. S. Kang, H. R. Shon and J. M. Sung. 2000. Studies on mycelial growth and artificial cultivation of Pleurotus eryngii. Kor. J. Mycol. 28, 73-80
  16. Yoshida, I., T. Kiho, S. Usui, M. Sakushima and S. Ukai. 1996. Polysaccharides in fungi. X X X VII. Immunomodulating activities of carboxymethylated derivatives of linear (1$\rightarrow$3)-alpha-D-glucans extracted from the fruiting bodies of Agrocybe cylindracea and Amanita muscaria. Biol. Phram. Bull. 19, 114-121 https://doi.org/10.1248/bpb.19.114
  17. Kim, H. K., J. S. Park, Y. S. Kim and Y. H. Park. 1989. Studies on the artifical cultivation of Agrocybe aegerita (Brig.) using sawdust substrate. Kor. J. Mycol. 17, 124-131
  18. Kim, S. Y., J. H. Son, H. C. Ha, H. W. Lee and J. S. Lee. 2002. Chemopreventive effects of the extracts from soybean fermented with basidiomycetes. Kor. J. Mycol. 30, 124-130 https://doi.org/10.4489/KJM.2002.30.2.124
  19. Lee, H. W., D. W. Lee, H. C. Ha, I. C. Jung and J. S. Lee. 2002. Antioxidant activities of the mycelium and culture broth of Phellinus igniarius and Agrocybe cylindracea. Kor. J. Mycol. 30, 37-43 https://doi.org/10.4489/KJM.2002.30.1.037
  20. Lee, I. K., B. S. Yun and I. D. Yoo. 1998. A nucleoside with lipid peroxidation inhibitory activity from Agrocybe cylindracea. Kor. J. Appl. Microbiol. Biotechnol. 26, 558-561
  21. Lee, J. K., Y. S. Choi and J. M. Sung. 2000. Investigation on cultural characteristics of mycelial growth by Cordyceps scarabaeicola. Kor. J. Mycol. 28, 81-87
  22. Lee, J. Y. 1998. Coloured Korean Mushrooms Ⅰ. Academic Press, 137

Cited by

  1. Development of bag culture medium of Auricularia auricula vol.12, pp.3, 2014, https://doi.org/10.14480/JM.2014.12.3.216
  2. Homology search of genus Pleurotus using an internal transcribed spacer region vol.24, pp.3, 2007, https://doi.org/10.1007/s11814-007-0070-2
  3. Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract vol.14, pp.2, 2009, https://doi.org/10.1007/s12257-008-0291-1
  4. Effects ofFomitopsis pinicola extracts on antioxidant and antitumor activities vol.12, pp.5, 2007, https://doi.org/10.1007/BF02931349
  5. A study of mycelial growth and exopolysaccharide production from a submerged culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor vol.28, pp.6, 2011, https://doi.org/10.1007/s11814-011-0109-2