생명과학회지 (Journal of Life Science)

  • 제14권4호
  • /
  • Pages.531-536
  • /
  • 2004
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

신령버섯 균사체 액체배양액이 고콜레스테롤혈증 흰쥐의 지질대사에 미치는 영향

Effects of Liquid Culture of Agaricus blazei Murill on Lipid Metabolism in Rats Fed Cholesterol Diet.

  • 고진복 (신라대학교 생명과학과) ;
  • 김재영 (부산가톨릭대학교 임상병리학과)
  • 발행 : 2004.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

신령버섯 균사체 액체배양액이 고콜레스테롤 식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자, 생후 7주령의 숫쥐에 표준식이를 급여한 정상군, 표준식이에 15% 돈지와 0.5% 콜레스테롤을 첨가한 식이를 급여한 대조군, 대조식이에 신령버섯 균사체 액체배양액을 음료수에 30% 및 40%로 혼합 급여한 군(30% 및 40% 신령버섯군) 등 4군으로 나누어 6주간 사육한 결과는 다음과 같다. 실험동물의 체중 증가량, 식이섭취량 및 식이효율은 대조군과 30%와 40% 신령버섯군이 유사하였다. 간, 신장 및 부고환지방의 무게는 대조군과 신령버섯군들이 비슷하였다 간의 콜레스테롤 농도, 간과 혈청의 중성지질 농도는 대조군과 신령버섯군들이 비슷한 수준으로 감소효과가 나타나지 않았다. 혈청의 총 콜레스테롤과 LDL-콜레스테롤 농도 및 동맥경화지수는 대조군에 비해 30% 및 40% 신령버섯군이 유의하게 감소되었다. 총콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 신령버섯군들이 유의하게 증가되었다. HDL-콜레스테롤 농도는 대조군과 신령 버섯군들이 비슷한 수준으로 신령 버섯섭취에 따른 영향은 나타나지 않았다. 분으로 배설된 지방은 대조군에 비해 40% 신령버섯군이 유의하게 증가되었다. 이상의 결과로 보아 고콜레스테롤 식이를 급여한 흰쥐에 신령버섯 균사체 액체배양액을 30%나 40% 급여시 혈청의 총 콜레스테롤, LDL-콜레스테롤 농도 및 동맥경화지수를 낮추고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시키는 것으로 나타났다.

The effects of liquid culture of Agaricus blazei Murill on the weight gains, serum and hepatic lipid concentrations were studied in male rats. Sprague-Dawley rats (7 weeks old) were given four different types of diets for 6 weeks, respectively: a normal diet group, a high fat control diet group (normal diet+15% lard +0.5% cholesterol), a 30% or 40% A. blazei diet groups (high fat control diet+30% or 40% A. blazei in water) according to the levels of A. blazei supplementation. The body weight gains, food intake, food efficiency ratios, and the liver, kidney, and epididymal fat pad weights of the rats fed 30% or 40% A. blazei diets were similar to those of the rats fed high fat control diet. The concentrations of serum total cholesterol, LDL-cholesterol and atherogenic index in rats fed the 30% or 40% A. blazei diets were significantly decreased compared with those of rats fed the high fat control diet. The HDL-cholesterol/total-cholesterol ratios of the rats fed 30% or 40% A. blazei diet were significantly increased compared with those of rats fed the high fat control diet. The fecal excretion of total lipid . in the rats fed 40% A. blazei diet was significantly increased compared with those of rats fed the high fat control diet. The concentrations of serum total lipid in the rats fed 40% A. blazei diet was significantly lower than that in the rats fed high fat control diet. But the concentrations of serum HDL-cholesterol, hepatic total cholesterol and triglyceride of rats fed the 30 or 40% A. blazei diets were similar to those of rats fed the high fat control diet. These results showed that the 30 or 40% A. blazei diets feeding decreased the total cholesterol, LDL-cholesterol and atherogenic index, and increased the HDL-cholesterol/total-cholesterol ratio in serum of rats.

키워드

참고문헌

  1. Anderson, J. W., A. E. Jones and S. Riddell-Mason. 1994. Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats. J. Nutr. 124, 78-83
  2. Bobek, P., L. Ozdin and S. Galbavy. 1998. Dose-and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats. Nutrition 14, 282-286 https://doi.org/10.1016/S0899-9007(97)00471-1
  3. Bobek. P., L. Ozdin and L. Kuniak. 1997. Effect of oyster mushroom and isolated $\beta$-glucan on lipid peroxidation and on the activities of antioxidative enzymes in rats fed the cholesterol diet. J. Nutr. Biochem. 8, 469-489 https://doi.org/10.1016/S0955-2863(97)00058-2
  4. Chang, H. L., G. R. Chao, C. C. Chen and J. L. Mau. 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chemistry 74, 203-207 https://doi.org/10.1016/S0308-8146(01)00127-3
  5. Cheung, P. C. K. 1996. The hypocholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutr. Res. 16, 1953-1957 https://doi.org/10.1016/S0271-5317(96)00218-7
  6. Chibada, I., K. Okumura, S. Takeyama and A. Kotera. 1969. Lentinacin, a new hypocholesterolemic substance in Lentinus edodes. Experientia 25, 1237-1242 https://doi.org/10.1007/BF01897467
  7. Choi, J. M. and S. J. Koo. 2000. Effects of $\beta$-glucan from Agaricus blazei Murill on blood glucose and lipid composition in db/db mice. Korean J. Food Sci. Technol. 32, 1418-1425
  8. Choi,. M. Y., S. S. Lim and T. Y. Chung. 2000. The effects of hot water soluble polysaccharides from Lentinus edodes on lipid metabolism in the rats fed butter yellow. J. Korean Soc. Food Sci. Nutr. 29, 294-299
  9. Dong, Q., J. Yao, X. T. Yang and J. N. Fang. 2002. Structural characterization of water-soluble $\beta$-D-glucan from fruiting bodies of Agaricus blazei Murr. Carbohydrate Research 337, 1417-1421 https://doi.org/10.1016/S0008-6215(02)00166-0
  10. Ebihara K. and B. O. Schneeman. 1989. Interaction of bile acids, phospholipids, cholesterol and triglycerides with dietary fiber in the small intestine of rats. J. Nutr. 119, 1100-1106
  11. Folch, J., M. Lees and G. S. H. Stanley. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem. 226, 497-509
  12. Fridwald, W. T., R. I. Levy and D. S. Fedreison. 1979. Estimation of concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499-508
  13. Frings, C. S. and R. T. Dunn. 1970. A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am. J. Clin. Path. 53, 89-91
  14. Haglund, O., R. Loustarinen, R. Wallin, I. Wibell and T. Saldeen. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. J. Nutr. 121, 165-172
  15. Herman, S., A. D. Seiaoetama, D. Karyadi and A. C. Beynen. 1991. Influence of background composition of the diet on th lipemic effect of fish oil versus oil in rats. J. Nutr. 121, 622-630
  16. Itoh, H., H. Amano and H. Noda. 1994. Inhibitory action of a (1$\rightarrow$6)-$\beta$-D-glucan-protein complex isolated from Agaricus blazei Muril on metha fibrosarcoma-bearing mice and its antitumor mechanism. Jpn. J. Pharmacol. 66, 265-271 https://doi.org/10.1254/jjp.66.265
  17. Kabir, Y., S. Kimura and T. Tamura. 1988. Dietary effects of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypotensive rats (SHR). J. Nutr. Sci. Vitaminol. 34, 433-438 https://doi.org/10.3177/jnsv.34.433
  18. Kailash, P. 1999. Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99, 1355-1362 https://doi.org/10.1161/01.CIR.99.10.1355
  19. Kawagish, H., R. Inagaki and T. Kanao. 1989. Fraction and antitumor activity of the water-insoluble residue of Agaricus blazei fruiting bodies. Carbohydrate Research 186, 267-273 https://doi.org/10.1016/0008-6215(89)84040-6
  20. Kawagish, H., T. Kanao, R. Inagaki and T. Mizuno. 1990. Formolysis of a potent antitumor (1-6)-$\beta$-D-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products. Carbohydrate Polymers 12, 393-403 https://doi.org/10.1016/0144-8617(90)90089-B
  21. Kim, B. K., G. G. Shin, B. S. Jeon and J. Y. Cha. 2001. Cholesterol-lowering effect of mushrooms powder Cholyperlipidemic rats. J. Korean Soc. Food Sci. Nutr. 30, 510-515
  22. Kim, G. H. and H. K. Han. 1998. The effect of mushroom extracts on carbon tetrachloride-induced hepatotoxicity in rats. J. Korean Soc. Food Sci. Nutr. 27, 326-332
  23. Kim. S. K., S. Rhee, I. K. Rhee, G. J. Joo and H. P. Ha. 1998. Effects of dietary xylooligosaccharide on lipid levels of serum in rats fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 27, 945-951
  24. Kinosian, B., H. Glick, L. Preiss and K. I. Puder. 1995. Cholesterol and coronary heart disease: predicting risk in men by changes in levels and ratios. J. Invest. Med. 43, 443-450
  25. Koh, J. B. 2002. Effect of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed high fat diet. Korean J. Nutrition 35, 414-420
  26. Koh, J. B. and M. A. Choi. 2001. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J. Nutrition 34, 265-270
  27. Koh, J. B. and M. A. Choi. 2003. Effect of Paecilomyes japonica on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 32, 238-243 https://doi.org/10.3746/jkfn.2003.32.2.238
  28. Menoli, R. C. R. N., M. S. Mantovani, L. R. Ribeiro, G. Speit and B. Q. Jordao. 2001. Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells. Mutation Research 496, 5-13 https://doi.org/10.1016/S1383-5718(01)00227-3
  29. Mizuno, T., T. Hagiwara, T. Nakamura, H. Ito, K. Shimura and T. Sumiya. 1990. Antitumor activity and some properties of water soluble polysaccharides from the fruiting body of Agaricus blazei Muril. Agric. Biol. Chem. 54, 2889-2896 https://doi.org/10.1271/bbb1961.54.2889
  30. Mizuno, T., R. Inagaki, T. Kanao and T. Hagiwara. 1990. Antitumor activity and some properties of water-insoluble hetero-glucans from 'Himematsutake' the fruiting body of Agaricus blazei Muril. Agric. Biol. Chem. 54, 2897-2905 https://doi.org/10.1271/bbb1961.54.2897
  31. Park, Y. J. and Y. J. Park. 1997. Effect of high fat and high cholesterol diet on kidney function. Korean J. Nutrition 30, 187-194
  32. Pella, D. and R. Rybar. 1997. Pleurotus ostreatus-effective hypolipidaemic agent. Atherosclerosis 134, 332-338 https://doi.org/10.1016/S0021-9150(97)89698-0
  33. Reeves, P. G., F. H. Nielsen and G. C. Fahey. 1993. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951
  34. Schectman, G., M Patsches and E. A. Sasse. 1996. Variability in cholesterol of measurements: comparison of calculated and direct LDL cholesterol determinations. Clin. Chem. 42, 732-737
  35. Sugiyama, K., T. Akachi and A. Yamakawa. 1995. Hypocholesterolemic action of eritadenine is mediate by a modification of hepatic phospholipid metabolism in rat. Nutr. 125, 2134-2140
  36. Sung, J. M., Y. B. Yoo and D. Y. Cha. 1998. Mushroom. pp. 3-10, Kyohaksa. Seoul
  37. Warnick, G. R., R. H. Knoopp, V. Fitzpatrick, L. Branson. 1990. Estimating low density lipoprotein cholesterol by the Friedewald equation is adequate for classifying patient on the basis of nationally recommended cut points. Clin. Chem. 36, 15-19
  38. Yamakoshi, J., M. K. Piskula, T. Izumi, K. Tobe, M. Saito, S. Kataoka, A. Obata and M. Kikchi. 2000. Isoflavone aglycone-rich extract without soy protein attenuates atherosclerosis development in cholesterol-fed rabbits. J. Nutr. 130, 1887-1893
  39. Yoshiaki, F., K. Hidekazu, O. Koichi, S. Ryo and E. Takusaburo. 1998. Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. Nippon Shokuhim. Kagaku Kaishi 45, 246-252 https://doi.org/10.3136/nskkk.45.246
  40. Zhang, X., J. A. Joles, H. A. Koomans, A. Van Tol and A. C. Beynen. 1992. Excessive cholesterolemic response in analbuminemic rats fed a cholesterol-rich diet containing casein. J. Nutr. 122, 520-527

피인용 문헌

  1. Effects of Pleurotus eryngii on Lipid Levels and Enzyme Activity in Male Rats Fed High Cholesterol Diets vol.16, pp.7, 2006, https://doi.org/10.5352/JLS.2006.16.7.1127
  2. Inhibition of α-Glucosidase by a Semi-Purified Ethyl Acetate Fraction from Submerged-Liquid Culture of Agaricus blazei Murill vol.21, pp.11, 2011, https://doi.org/10.5352/JLS.2011.21.11.1579
  3. Effect of Hen Egg Supplementation on Blood Lipid Profile and Fecal Bile Acid of C57BL/6 Mouse Fed Normal and High Cholesterol Diet vol.31, pp.2, 2011, https://doi.org/10.5851/kosfa.2011.31.2.250
  4. Effect of Feeding Eggs on Levels of Serum Lipid, Protein, and Enzyme Activities in Rats vol.17, pp.4, 2007, https://doi.org/10.5352/JLS.2007.17.4.529