Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Properties of Non-Cariogenicity Sugar Produced by Alkalophilic Bacillus sp. S-1013

  • Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University) ;
  • Kim, Sun-Sook (Department of Dental Hygienics, Suwon Women's College) ;
  • Lee, Kap-Sang (College of Life Science and Natural Resources, Wonkwang University)
  • Published : 2004.08.01
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Abstract

The NCS(Non-Cariogenicity Sugar) from Bacillus sp. S-1013 was purified by cold acetone and methanol precipitation, and DEAE-cellulose ion-exchange and Sephadex G-100 column chromatographies, to yield an amorphous yellow syrup. The melting point and $[\alpha]_D^{20}$ were 155-$157^{\circ}C$ and +53, respectively. Instrumental analyses such as FT-IR, $^1H-NMR, and ^{13}C-NMR$ showed that the NCS contained an O-H group, C-H, C=O, $NH_2$, anomeric carbon, anomeric proton, N-acetylgalactose, fucose, and neuramic acid, thus, the NCS was determined to be a trisaccharide of Fuc($1\longrightarrow4$)GalNAc($2\longrightarrow6$) NeuAc.

Keywords

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