Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Improved Plant Growth from Seed Bacterization Using Siderophore Overproducing Cold Resistant Mutant of Pseudomonas fluorescens

  • Katiyar, Vandana (Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant Univ. of Ag. & Tech.) ;
  • Goel, Reeta (Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant Univ. of Ag. & Tech.)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The cold resistant mutants of P. fluorescens strain $PRS_{9}$ and ATCC13525 were developed which could grow equally well at $28^{\circ}C$ and $10^{\circ}C$. All the mutants were tested for siderophore production, of which $CRPF_9$ (ATCC13525 mutant) was selected, as there was a 16.8-fold increase when compared to its wild-type. Under in vitro conditions, $CRPF_9$ showed better growth promotion both in wheat (29.1% increase in root length) and mung bean (51.5% increase in root length) at $10^{\circ}C$. Greenhouse trials showed a significant increase in root (13.84cm) and shoot (15.0cm) length of $CRPF_9$-treated mung bean seeds, indicating increased rhizocompetence of the mutant. Ferric citrate was a better iron source than ferric hydroxide for plant growth.

Keywords

References

  1. Z. Naturforsch. v.52c Siderophores of fluorescent pseudomonads Budzikiewicz, H.
  2. Appl. Environ. Microbiol. v.59 A method for the detection of pseudobactin, the siderophore produced by a plant-growth-promoting Pseudomonas strain, in the barley rhizosphere Buyer, J. S.;M. G. Kratzke;L. J. Sikora
  3. Plant Physiol. v.87 Utilization of microbial siderophores in iron acquistion by oats Crowley, D. E.;C. P. P. Reid;P. J. Szaniszlo https://doi.org/10.1104/pp.87.3.680
  4. Can. J. Microbiol. v.41 The enhancement of plant growth by freeliving bacteria Glick, B. R. https://doi.org/10.1139/m95-015
  5. Biochemical and Genetic Mechanisms Used by Plant Growth-Promoting Bacteria Glick, B. R.;C. C. Patten;G. Holguin;D. M. Penrose
  6. Asian J. Microbiol. Biotechnol. Environ. Sci. v.4 Detection and characterization of cold resistant mutants of fluorescent pseudomonads by ARDRA Goel, R.;S. Khanna;M. Kumar
  7. Univ. Calif. Coll. Agric. Exp. Stud. Circ. The water culture method for growing plants without soil Hoagland, D. R.;D. I. Arnon
  8. Mol. Ecol. v.3 Fluorescent Pseudomonas species categorized by using polymerase chain reaction(PCR) restriction fragment analysis of 16S rDNA Laguerre,G.;L.Rigottier-Gois;P.Lemanceau https://doi.org/10.1111/j.1365-294X.1994.tb00126.x
  9. Arch. Microbiol. v.174 Pyoverdines: Pigments, siderophores and potential taxonomic markers of fluorescent Pseudomonas sp Meyer, J. M. https://doi.org/10.1007/s002030000188
  10. J. Gen. Microbiol. v.160 The fluorescent pigment of Pseudomonas fluorescens: Biosynthesis, purification and physiochemical properties Meyer, J. M.;M. A. Abdallah
  11. Appl. Environ. Microbiol. v.68 Siderophore typing, a powerful tool for the identification of fluorescent and non fluorescent pseudomonads Meyer, J. M.;V. Geoffroy;N. Baida;L. Gardan;D. Izard;P. Lemanceau;W. Achouak;N. Palleroni https://doi.org/10.1128/AEM.68.6.2745-2753.2002
  12. FEMS Microbiol. Ecol. v.34 Fitness in soil and rhizosphere of Pseudomonas fluorescens C7R12 comapred with a C7R12 mutant affected in pyoverdine synthesis and uptake Mirleau, P.;S. Delorme;L. Philippot;J. M. Meyer;S. Mazurier;P. Lemanceau https://doi.org/10.1111/j.1574-6941.2000.tb00752.x
  13. J. Biotechnol. v.75 Development of a cold resistant mutant of plant growth promoting Pseudomonas fluorescens and its functional characterization Mishra, M.;R. Goel https://doi.org/10.1016/S0168-1656(99)00137-6
  14. Plant Pathol. v.48 Protection of cucumber against Pythium root rot by fluorescent pseudomonads: Predominant role of induced resistance over siderophores and antibiosis Ongena, M.;F. Daayf;P. Jacques;P. Thonart;N. Benhamou;T. C. Paulitz https://doi.org/10.1046/j.1365-3059.1999.00315.x
  15. Plant Cell Environ. v.26 Tales from the underground: Molecular plant-rhizobacteia interactions Persello-Cartieaux, F.;L. Nussaume;C. Robaglia https://doi.org/10.1046/j.1365-3040.2003.00956.x
  16. Ind. J. Microbiol. v.38 Seed and root extracts in chemotaxis, agglutination, adherence, and root colonization of soybean (Glycine max) by fluorescent pseudomonads Rao, Ch. V. S.;B. N. Johri
  17. Science v.216 Diseasesuppressive soil and root-colonizing bacteria Schroth, M. N.;J. G. Hancock https://doi.org/10.1126/science.216.4553.1376
  18. Modern Soil Microbiology The rhizospher as a habiat for soil microorganisms Sorenson, J.;J. D. van Elsas(ed.);J. T. Trevors(ed.);E. M. H. Wellington(ed.)
  19. Can. J. Microbiol. v.41 Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 Sun, X.;M. Griffith;J. J. Pasternak;B. R. Glick https://doi.org/10.1139/m95-107
  20. Plant Cell Environ. v.16 Evidence for direct utilization of siderophore, ferroxamine B in axenically grown cucumber Wang, Y.;H. N. Brown;D. E. Crowley;P. J. Szaniszlo https://doi.org/10.1111/j.1365-3040.1993.tb00906.x
  21. Phytopathology v.73 Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads Weller, D. M.;R. J. Cook https://doi.org/10.1094/Phyto-73-463