Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Improvement Effect of Capsaicin Against Orotic Acid-Induced Fatty Liver in Rats

Orotic Acid-유발 지방간에 미치는 Capsaicin의 개선효과

  • 전방실 (동아대학교 응용생명공학부) ;
  • 차재영 (동아대학교 응용생명공학) ;
  • 유기수 (동아대학교 의과대학 해부학교) ;
  • 조영수 (동아대학교 응용생명공학부)
  • Published : 2004.06.01
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Abstract

The effect of capsaicin (0.02%) on the concentrations of liver and serum lipids, GOT and GPT activities, and hepatocyte morphology in male Sprague Dawley rats fed diets with or without erotic acid (1.0%) for 28 days was studied. Liver triglyceride concentration was increased by approximately 4.7-fold after erotic acid-feeding, wherease simultaneous feeding of capsaicin and erotic acid reduced its concentration by 38%. Orotic acidfeeding elevated liver cholesterol concentration, but an addition of capsaicin to the erotic acid diet resulted in the significant decrease in liver cholesterol. Capsaicin alone did not affect the liver triglyceride and cholesterol concentrations. Reduction of the serum concentrations of triglyceride and cholesterol by capsaicin was also observed in rats fed diets with erotic acid. The serum GOT and GPT activities were not significantly different among the experimental groups. Liver tissue morphology showed that the hepatocytes of erotic acid-feeding rats were a typical fatty liver with numerous fat droplets, whereas simultaneous feeding of capsaicin with erotic acid reduced the size and numbers of fat droplets. The present study demonstrated that the diet containing 0.02% capsaicin can prevent the erotic acid-induced fatty liver.

Orotic acid(OA)-유발 지방간 흰쥐의 혈청 및 간장의 지질농도, 임상생화학적 및 조직형태학적 변화에 미치는 cap-saicin(CS)성분의 영향을 검토하였다. 기본식이에 OA 1%, CS 0.02% 및 OA와 CS를 각각 1%와 0.02% 수준으로 첨가하여 4주간 섭취 시켰다. 간장 중성지방 농도는 대조군에 비해 약 4.7배 현저히 증가하여 지방간이 유발되었으나, OA十CS 동시투여에 의해서는 간장 중성지방 농도가 OA군에 비해 38% 감소하여 지방간이 개선된 것으로 나타났다. OA 식이에 의해 간장 콜레스테롤 농도가 증가하였으나, capsaicin 동시투여에 의해 감소하였다. Capsaicin 단독 투여는 간장 중성지방 및 콜레스테롤 농도에 영향을 미치지 못하였다. Capsaicin 투여에 의한 혈청 중성지방 및 콜레스테롤 농도의 감소는 OA 첨가 또는 무첨가 식이군에서 모두 관찰되었다. 간기능 지표 효소인 혈청 GOT 및 GPT 활성은 각 실험군간에 차이가 나타나지 않았다. 간조직의 형태학적 관찰 결과, 대조군과 CS군은 정상적인 간소견을 보였으나 OA군의 간세포에는 소포성 지방 변성이 소엽 중심대로 주로 나타나 있고, 크고 많은 지방세포들이 균일하게 배열되어 있었다. 그러나 OA+CS군의 간세포들은 손상된 세포가 재생될 뿐 아니라 소포성 지방세포들의 크기와 수가 줄어들고 있음을 확인하였다. 이상의 실험 결과에서, capsaicin 0.02% 농도는 OA-유발 지방간을 개선시키는 효과가 있는 것으로 판단되어진다.

Keywords

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