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Systematics of Thuja Based on Leaf Monoterpenoids

측백나무속(Thuja)의 잎에 합유된 Monoterpenoids 분석을 통한 종간의 화학분류학적 연구

  • 조규갑 (경남대학교 자연과학대학 생명과학부) ;
  • 김종희 (경남대학교 자연과학대학 생명과학부)
  • Published : 2004.06.01

Abstract

The compositions of the leaf monoterpenoids in 7 species of Thuja (Thuja orientalis, T. orientalis 'Avrea Nana', T. orientalis cv. compacts, T. occidentalis, T. occidentalis 'Boothii', T. occidentalis 'Pumila', T. occidentalis 'Tiny Tim') were analyzed by GC-MS and compared between species. These Thuja contains 30 compounds and α -pinene, camphene, sabinene, myrcene, limonene, bonyl acetate, γ-terpinene, α -terpinenyl acetatee are occurred in these all species. Compounds in these leaf extracts are remarkably different between species. The simplest monoterpenoid (11 compounds) was found in T. orientalis 'Avrea Nana' the most complex monoterpenoid was in T. occidentalis 'Pumila'(26 compounds). Based on these data, similarities are computed using presence/absence matching by PAUP (Phylogenetic Analysis Using Parsimony). It appears that four taxa are present within these Thuja plants. The minimum spanning network reveals that Thuja occidentalis and Thuja occidentalis 'Boothii' were the most similar compounds.

본 실험에서는 Thuja 속 7종(Thuja orientalts, T. orientalis 'Avrea Nana', T. orientalis cv. compacts, T. occidentalis, T. occidentalis 'Boothii', T. occidentalis 'Pumila', T. occidentalis 'Tiny Tim')의 침엽에 함유된 monoterpenenoids를 GC-MS로 분석하여 종간을 비교하였다. 분석결과 총 30종류의 성분이 검출되었는데 그 중 α-pinene, camphene, sabinene, myrcene, limonene, bonyl aetate, γ-terpinene, α-terpinenyl acetate는 7종 식물모두에서 검출되었다. Thuja속 7종에서 추출된 monoterpenenoids는 종간에 차이가 나타났으며 Thuja orientalis 'Avrea Nana'(11 Compounds)는 가장 작은 성분이 나타났고 Thuja occidentalis 'Pumila'(26 Compounds)는 가장 많은 종류의 성분이 검출되었다. 검출된 성분을 토대로 종간의 유사성을 PAUP(Phylogenetic Analysis Using Parsimony) 프로그램으로 분계분석을 하였다. 분계도는 4가지 형태가 나타났고 Thuja occidentalis와 Thuja occidentalis 'Boothii'가 가장 유사한 유연관계를 보였다.

Keywords

References

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