16S rDNA 클론 Libraries를 이용한 치근단 농양 병소의 세균 동정

Identification of Bacteria from Periapical Abscess Using 16S rDNA Clone Libraries.

  • 유소영 (조선대학교 치과대학 구강생화학교실) ;
  • 김미광 (조선대학교 치과대학 구강생화학교) ;
  • 김화숙 (조선대학교 치과대학 구강생화학교) ;
  • 황호길 (조선대학교 치과대학 구강생물학 연구) ;
  • 김평식 (조선대학교 치과대학 구강생물학 연구) ;
  • 임성훈 (조선대학교 치과대학 구강생물학 연구) ;
  • 오상호 (조선대학교 치과대학 구강생물학 연구) ;
  • 민정범 (조선대학교 치과대학 구강생물학 연구) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • 발행 : 2004.06.01

초록

Molec-ular analysis was performed on the microflora found In the necrotic pulpal tissue collected from 5 infected root canals that were diagnosed as a periapical abscess. 16S rRNA coding gene (rDNA) library construction and sequencing were performed in order to identify the microflora, The 16S rDNA sequences from 278 clones were identified by a comparison with the database sequence in GenBank. Three phylum and 31 species, which were related to the oral microflora, were identified from the 3 samples (No. 87, 105, and 115). Dialister invisus (5.6%), Peptostreptococcus micron (18.3%), and Veillonella sp. (3.3%) were the organism present in all tee samples. Lac-tobacillusfementum (2.8%),Eubacterumsp./E. infirmum (6.7%), Shuttleworthiasatelles (3.9%), Psudorarnihacfer alactoiyticus (13.3%), Bulleidia moorei (2.8%), and Prevotella denticola (1.1%) were found in two samples. Two phylum and low species of environmental microflora were identified from 2 samples (No.95 and 101). The reason for this might be contamination of the samples with dental water. These results showed that molecular analysis could reveal more diverse microflora that are associated with endodontic infections than that revealed by conventional cultural methods. In addition, these results may of for the basic data to epidemiological studies related with endodontic infection.

키워드

참고문헌

  1. J. Periodontol. v.68 Putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis Albandar, J. M.;L. J. Brown;H. Loe https://doi.org/10.1902/jop.1997.68.10.973
  2. J. Int. Endodont. v.31 Microorganisms in closed periapical lesions Abou-Rass, M.;G. Bogen https://doi.org/10.1046/j.1365-2591.1998.t01-1-00088.x
  3. J. Endodont. v.25 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and polymerase chain reaction for differentiation of Prevotella intermedia and Prevotella nigrescens Baumgartner, J. C.;K. S. Bae;T. Xia;J. Whitt;L. L. David https://doi.org/10.1016/S0099-2399(06)81164-7
  4. J. Endodont. v.25 Association of black-pigmented bacteria with endodontic infections Baumgartner, J. C.;B. J. Watkins;K .S. Bae;T. Xia https://doi.org/10.1016/S0099-2399(99)80268-4
  5. Int. J. Syst. Evol. Microbiol. v.53 Dialister invisus sp. nov., isolated from the human oral cavity Downes, J.;M. Munson;W. G. Wade https://doi.org/10.1099/ijs.0.02640-0
  6. Int. J. Syst. Evol. Microbiol. v.52 Shuttleworthia satelles gen. nov., sp. nov., isolated from the human oral cavity Downes, J.;M. A. Munson;D. R. Radford;D. A. Spratt;W. G. Wade https://doi.org/10.1099/ijs.0.02122-0
  7. Oral Microbiol. Immunol. v.18 Molecular characterization of the presence of Eubacterium spp and Streptococcus spp in endodontic infections Found, A. F.;K. Y. Kum;M. L. Clawson;J. Barry;C. Abenoja;Q. Zhu;M. Caimano;J. D. Radolf https://doi.org/10.1034/j.1399-302X.2003.00077.x
  8. J. Endodont. v.21 Microbiological and immunological study of endodontic-periodontic lesions Kurihara, H.;Y. Kobayashi;I. A. Francisco;O. Isoshima;A. Nagai;Y. A. Murayama https://doi.org/10.1016/S0099-2399(06)81115-5
  9. Oral Microbiol. Immunol. v.16 Microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro Lana, M. A.;A. P. Ribeiro-Sobrinho;R. Stehling;G. D. Garcia;B. K. Silva;J. S. Hamdan;J. R. Nicoli;M. A. Carvalho;L. D. Farias https://doi.org/10.1034/j.1399-302x.2001.016002100.x
  10. Nucleic Acids Res. v.24 The ribosomal database project (RDP) Maidak, B. L.;G. J. Olsen;N. Larsen;R. Overbeek;M. J. McCaughey;C. R. Woese https://doi.org/10.1093/nar/24.1.82
  11. J. Dent. Res. v.81;82 Molecular and cultural analysis of the microflora associated with endodontic infections Munson, M. A.;T. Pitt-Ford;B. Chong;A. Weightman;W. G. Wade
  12. J. Bacteriol. v.183 Bacterial diversity in human subgingival plaque Paster, B. J.;S. K. Boches;J. L. Galvin;R. E. Ericson;C. N. Lau;V. A. Levanos;A. Sahasrabudhe;F. E. Dewhirst https://doi.org/10.1128/JB.183.12.3770-3783.2001
  13. J. Clin. Microbiol. v.39 Molecular identification of microorganisms from endodontic infections Rolph, H. J.;A. Lennon;M. P. Riggio;W. P. Saunders;D. MacKenzie;L. Coldero;J. Bagg https://doi.org/10.1128/JCM.39.9.3282-3289.2001
  14. Oral Sug. Oral Med. Oral Pathol. Oral Radiol. Endod. v.94 Dialister pneumosints can be a suspected endodontic pathogen Siqueira, J. F. Jr.;I. N. Rocas https://doi.org/10.1067/moe.2002.125202
  15. J. Endodon. v.27 Molecular detection of black-pigmented bacteria in infections of endodntic origin Siqueira, J. F. Jr;I. N. Rocas;J. C. Oliveira;K. R. Santos https://doi.org/10.1097/00004770-200109000-00002
  16. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. v.89 Checkerboard DNA-DNA hybridization analysis of endodontic infections Siqueira, J. F. Jr.;I. N. Rocas;R. Souto;M. de Uzeda;A. P. Colombo https://doi.org/10.1067/moe.2000.106576
  17. Clin. Infect. Dis. v.4 Oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment Tanner, A.;N. Stillman