Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Bacteria from Periapical Abscess Using 16S rDNA Clone Libraries.

16S rDNA 클론 Libraries를 이용한 치근단 농양 병소의 세균 동정

  • 유소영 (조선대학교 치과대학 구강생화학교실) ;
  • 김미광 (조선대학교 치과대학 구강생화학교) ;
  • 김화숙 (조선대학교 치과대학 구강생화학교) ;
  • 황호길 (조선대학교 치과대학 구강생물학 연구) ;
  • 김평식 (조선대학교 치과대학 구강생물학 연구) ;
  • 임성훈 (조선대학교 치과대학 구강생물학 연구) ;
  • 오상호 (조선대학교 치과대학 구강생물학 연구) ;
  • 민정범 (조선대학교 치과대학 구강생물학 연구) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • Published : 2004.06.01
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Abstract

Molec-ular analysis was performed on the microflora found In the necrotic pulpal tissue collected from 5 infected root canals that were diagnosed as a periapical abscess. 16S rRNA coding gene (rDNA) library construction and sequencing were performed in order to identify the microflora, The 16S rDNA sequences from 278 clones were identified by a comparison with the database sequence in GenBank. Three phylum and 31 species, which were related to the oral microflora, were identified from the 3 samples (No. 87, 105, and 115). Dialister invisus (5.6％), Peptostreptococcus micron (18.3％), and Veillonella sp. (3.3％) were the organism present in all tee samples. Lac-tobacillusfementum (2.8％),Eubacterumsp./E. infirmum (6.7％), Shuttleworthiasatelles (3.9％), Psudorarnihacfer alactoiyticus (13.3％), Bulleidia moorei (2.8％), and Prevotella denticola (1.1％) were found in two samples. Two phylum and low species of environmental microflora were identified from 2 samples (No.95 and 101). The reason for this might be contamination of the samples with dental water. These results showed that molecular analysis could reveal more diverse microflora that are associated with endodontic infections than that revealed by conventional cultural methods. In addition, these results may of for the basic data to epidemiological studies related with endodontic infection.

Keywords

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