Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimal Condition for Citric Acid Production from Milk Factory Waste Water by Using the Immobilized Cells of Aspergillus niger

고정화 Aspergillus niger 세포를 이용한 우유공장 폐수로부터 구연산 생산의 최적 조건

  • 이용희 (동의대학교 기초과학연구소 및 자연과학대학 화학전공) ;
  • 서명교 (동의공업대학 환경정보시스) ;
  • 노호석 (하창식품(주) ;
  • 이동환 (동의대학교 기초과학연구소 및 자연과학대학 화학전) ;
  • 정경태 (동의대학교 자연과학대학 생명응용과학전) ;
  • 정영기 (동의대학교 자연과학대학 생명응용과학전공)
  • Published : 2004.06.01
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Abstract

Immobilized cells of Aspergillus niger was employed to produce citric acid by fermentation of milk factory waste water. A. niger ATCC 9142 as a citric acid production strain was cultured for 3 days and was entrapped with Ca-alginate bead about 2.5∼3.5 mm. The optimal pH and temperature were estimated to be 3.0 and $30^{\circ}C$, respectively. Dilution rate for fermentation was calculated to be $0.025 h^{－1}$ . Maximum amount of citric acid was obtained at 4.5 g/$\ell$ with the optimized fermentation condition. The yield of citric acid produced by immobilized A. niger ATCC 9143 was 70.3%. The yield was increased by 20% with immobilized cell, compared to that of the shake flask culture. Hence, the milk factory waste water is worthy to be used for the substrate of citric acid fermentation.

고정화된 Aspenillus niger 세포가 우유공장폐수에서 발효로 구연산을 생산하는데 이용되었다. 구연산 생산균주로서 A. niger ATCC 9142가 3일간 전배양되었으며, 약 2.5∼3.5 mm Ca-alginate beads 로 포획되었다. 최적의 pH와 온도는 각각 3.0과 $30^{\circ}C$였다. 발효에 대한 최적 희석률은 $0.025h^{－1}$ 인 것으로 계산되었다. 구연산의 최대 생산량은 최적화된 발효조건과 함께 4.5 g/$\ell$에서 얻어졌다. 고정화된 Aspergillus niger ATCC 9142에 의해 생산된 구연산의 수율은 70.3%였다. Shake-flask배양실험에 비해 연속식 배양실험 결과가 고정화 세포에 의해 20%증가되었다. 따라서 우유공장폐수는 구연산 발효기질로서의 이용가치가 있다고 볼 수가 있겠다.

Keywords

References

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