The pH as a Control Parameter for Oxidation-Reduction Potential on the Denitrification by Ochrobactrum anthropi SY 509

  • Kim, Sung-Hong (Bio-Pharmaceutical Research Team, SK Corporation/School of Chemical Engineering, Seoul National University) ;
  • Song, Seung-Hoon (Interdisciplinary Program for Biochemical Engineering and Biotechnology) ;
  • Yoo, Young-Je (Interdisciplinary Program for Biochemical Engineering and Biotechnology and School of Chemical Engineering, Seoul National University)
  • Published : 2004.06.01

Abstract

The pH as a control parameter for oxidation-reduction potential (ORP) was investigated through the denitrification by Ochrobactrum anthropi SY509 under non-growing condition. The optimal pH of nitrate reductase was 7.0, and the minimal ORP level was -250 mV for the denitrification under aerobic condition. In the case of anaerobic condition, the optimal pHs of nitrate and nitrite reductase were shifted to 10.0 and 9.0, respectively, and the minimal ORP levels of nitrate and nitrite reductase were decreased to -370 mV and -340mV, respectively. In the case of alkaline pH and anaerobic condition, the denitrification efficiency of nitrate was increased up to about 2-fold over that of neutral pH and anaerobic condition. Therefore, the combined control of pH and ORP in the anaerobic condition is shown to be an important parameter in the biological denitrification process.

Keywords

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