High Copy Rme1p Suppresses Iron-Induced Cell Growth Defect of Saccharomyces cerevisiae

  • Park, Yong-Sung (School of Life Sciences and Biotechnology, Korea University) ;
  • Yun, Cheol-Won (School of Life Sciences and Biotechnology, Korea University) ;
  • Kong, Jae-Yang (Korea Research Institute of Chemical Technology) ;
  • Kim, Tae-Hyoung (Chosun University School of Medicine, Department of Biochemistry & Molecular Biology) ;
  • Sung, Ha-Chin (School of Life Sciences and Biotechnology, Korea University)
  • 발행 : 2004.06.01

초록

In the yeast Saccharomyces cerevisiae, iron can be toxic. Because of this phenomenon, its metabolism of iron is strictly regulated. We have constructed a model system in which cell growth is defected during periods of iron over-load. When $Aft1-1^{up}$ protein was overexpressed with Ga110 promoter, a galactose inducible promoter, cell growth was defected and levels of CLN2 transcript decreased. However transcript levels of AFT1 and FET3 genes increased over time in a consistent manner throughout the course of $AFT1-1^{up}$ overexpression. We have screened to find genes to suppress cell growth defect by iron overload with YEp-derived high copy yeast genomic DNA library and found that high copy of Rmelp suppressed cell growth defects. Rme1p has been known as an activator protein of CLN2 gene expression. Taking these results together, we suggest that the yeast cell cycle is arrested at the $G_1$, phase by iron overload via Cln2p.

키워드

참고문헌

  1. Iron Transport and Storage. Cammack,R.;J.M.Wrigglesworth;H.Baum;Ponka,P.(ed.);Shulman,H.M.(ed.);Woodworth,R.C.(ed.)
  2. Genes Dev. v.5 The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-alpha 2 Covitz,P.A.;I.Herskowitz;A.P.Mitchell https://doi.org/10.1101/gad.5.11.1982
  3. Genes Dev. v.7 Repression by the yeast meiotic inhibitor RME1 Covitz,P.A.;A.P.Mitchell https://doi.org/10.1101/gad.7.8.1598
  4. Genetics v.138 Requirement for RGR1 abd SIN4 in RME1-dependent repression in Saccharomyces cerevisiae Covitz,P.A.;A.Song;A.P.Mitchell
  5. Curr. Opin. Cell Biol. v.7 Starting the cell cycle: What's the point? Cross,F.R. https://doi.org/10.1016/0955-0674(95)80062-X
  6. Mol. Cell. Biol. v.10 Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae Dancis,A.;R.D.Klausner;A.G.Hinnebusch;J.G.Barriocanal https://doi.org/10.1128/MCB.10.5.2294
  7. J. Biol. Chem. v.269 The FET4 gene encodes the low affinity fe(II) transport protein of Saccharomyces cerevisiae Dix,D.R.;J.T.Bridgham;M.A.Broderius;C.A.Byersdorfer;D.J.Eide
  8. J. Biol. Chem. v.272 Characterization of the FET4 protein of yeast. Evidence for a direct role in the transport of iron Dix,D.R.;J.T.Bridgham;M.A.Broderius;D.J.Eide https://doi.org/10.1074/jbc.272.18.11770
  9. Mol. Genet. Genomics v.266 Rme1, which controls CLN2 expression in Saccharomyces cerevisiae, is a nuclear protein that is cell cycle regulated Frenz,L.M.;A.L.Johnson;L.H.Johnston https://doi.org/10.1007/s004380100515
  10. Free Radicals in Biology and Medicine.(2 nd Ed.) Halliwell,B.;J.M.Gutteridge
  11. J. Biol. Chem. v.271 Transcriptional remodeling and G1 arrest in dioxygen stress in Saccharomyces cerevisiae Lee,J.;A.Romeo;D.J.Kosman https://doi.org/10.1074/jbc.271.40.24885
  12. Nature v.319 Activation of meiosis and sporulation by repression of the RME1 product in yeast Mitchell,A.P.;I.Herskowitz https://doi.org/10.1038/319738a0
  13. EMBO J. v.17 Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1up yeast Philpott,C.C.;J.Rashford;Y. Yamaguchi-Iwai;T.A.Roualt;A.Dancis;R.D.Klausner https://doi.org/10.1093/emboj/17.17.5026
  14. Nucleic Acids Research v.26 Genomic footpriting of the yeast zinc finger protein Rme1p and its roles in repression of the meiotic activator IME Shimizu,M.;W.Li;P.A.Covitz;M.Hara;H.Shindo;A.Mitchell https://doi.org/10.1093/nar/26.10.2329
  15. EMBO J. v.14 AFT-1: A mediator of iron regulated transcriptional control in Saccharomyces cerevisiae Yamaguchi-Iwai,Y.;A.Dancis;R.D.Klausner
  16. EMBO J. v.15 Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in teast Yamaguchi-Iwai,Y.;R.Stearman;A.Dancis;R.D.Klausner