생명과학회지 (Journal of Life Science)

  • 제14권3호
  • /
  • Pages.509-513
  • /
  • 2004
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

식용 및 약용 산채류로부터 트롬빈 저해물질의 탐색

Inhibitory Activities of Edible and Medicinal Herbs Against Human Thrombin

  • 권정숙 (안동대학교 식품영양학과) ;
  • 권윤숙 (안동대학교 식품영양학과) ;
  • 김영숙 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 진익렬 (경북대학교 미생물학과) ;
  • 류기철 (㈜대평 천연물신소재 개발연구소) ;
  • 손호용 (안동대학교 식품영양학과)
  • 발행 : 2004.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

혈전 관련 질환 예방 및 혈류개선 기능성 식품의 개발을 목표로, 식용 및 약용 산채류 55종의 메탄을 추출물을 조제한 후 트롬빈 저해활성을 검색하였다. 그 결과, 대조군으로 사용된 아스피린보다 우수한 항혈전 활성을 나타내는 붉나무, 사삼, 우산나물, 쥐다래, 참나물, 할미밀망, 큰까치수영, 첫버들 추출물 등 8종을 선별하였으며, aPTT, 혈전 용해활성, 열 안정성, 기타 소화효소 저해활성 등을 평가하며 참나물, 큰까치수영 및 갯버들 추출물을 최종 선별하였다. 참나물의 경우 열안정성이 낮은 반면 소화효소 비저해로 인해 그 이용성이 높으며, 큰 까치수영 및 갯버들의 경우 부분적인 소화효소 저해능이 있으나 우수한 항혈전활성 및 열 안정성을 나타내어 다양한 식품소재로 개발이 가능함을 확인하였다.

Inhibitory activities of 58 methanol extracts, which were prepared from different parts of 55 kinds of edible and medicinal plants, against human thrombin were evaluated. Analysis of the anti-coagulation activity determined by thrombin time enabled us to select 8 herb extracts as possible sources of anti-thrombosis agent. Further analysis of anti-thrombosis activity determined by activated partial thromboplastin time and fibrinolytic activity, and heat stability and inhibition against other proteolytic digestive enzymes provided new information that Pimpinella brachycarpa, Lysimachia clethroides, and Salix gracilistyla could be used as a potential anti-thrombosis agent. Our results suggest that edible and medicinal plants could be the potential source of thrombin inhibitor.

키워드

참고문헌

  1. Archs. Biochem. Biophys. v.40 The fibrin plate method for estimating fibrinolytic activity Astrup,T.;S.Mullertz https://doi.org/10.1016/0003-9861(52)90121-5
  2. Biochemisrty (Moscow) v.67 Blood coagulation Butenas,S.;K.G.Mann https://doi.org/10.1023/A:1013985911759
  3. Thrombosis Research v.105 Pharmacodynamics of the anticoagulant activity(APTT) of an algal polysaccharide Cattaneo,F.;F.Trento;R.Pescador;R.Porta;L.Furro https://doi.org/10.1016/S0049-3848(02)00047-6
  4. Kor. J. Pharmacogn v.30 Evaluation of anticoagulant and fibrinolytic activities from crude extracts of insects Hahn,B.S.;S.Wu;S.W.Kim;Y.S.Kim
  5. Korean J. Food Sci. Technol. v.33 Antimicrobial activity of Lysimachia clethroides Duby extracts on food-borne microorgansims Han,J.S.;D.H.Shin
  6. Korean J. Soc. Food Cookery Sci. v.17 Analysis of Umbelliferaceae wild plants and antioxidative activity of pork meat products added with wild plants Heo,S.J.;M.Y.Yang;E.J.Cho
  7. Thrombosis Research v.86 Thrombin interaction with fibrin polymerization sites Hsieh,K.H. https://doi.org/10.1016/S0049-3848(97)00073-X
  8. A study on causes of death for 1999 Korea National Statistical Office
  9. Planta Med. v.67 Effect of salicis cortex extract on human platelet aggregation Krivoy,N.;E.Pavlotzky;S.Chrubasik;E.Eisenberg;G.Brook https://doi.org/10.1055/s-2001-12000
  10. Neutraceuticals & Food v.7 Induction of quinone reductase, an anticarcinogenic marker enzyme, by medicinal herb extracts Kwon,C.S.;J.H.Kim;K.H.Son;Y.K.Kim;J.S.Lee;J.K.Lim;J.S.Kim https://doi.org/10.3746/jfn.2002.7.4.358
  11. J. Korean Soc. Agri. Chem Biotechnol. v.42 Inhibitory activites of Basidiomycetes on proly1 endopeptidase, acetylcholine esterase and coagulation Lee,H.J.;J.S.Kim;G.Y.Heo;K.B.Lee;I.K.Rhee;K.S.Song
  12. Korean J. Food Sci. Technol. v.34 Studies on proteolytic and fibrinolytic activity of Bacillus subtilis JM-3 isolated from anchovy sauce Lee,S.S.;S.M.Kim;U.Y.Park;H.Y.Kim;I.S.Shin
  13. Illustrated flora of Korea Lee,T.B.
  14. Se Pu. v.19 Determination of salicin in extract of willow bark by high performance liquid chromatography Li,L.S.;W.D.Huang;Q.He;S.Ye
  15. Korean J.Soc. Food Cookery Sci. v.18 Nitrite scavenging effect of Umbelliferaceae Noh,K.S.;M.Y.Yang;E.J.Cho
  16. Thrombosis Research v.106 Direct thrombin inhibitors Weitz,J.I.;M.Crowther https://doi.org/10.1016/S0049-3848(02)00093-2
  17. Kor. J.Appl. Microbiol. Bioeng. v.10 Trypsin inhibitor from Streptomyces sp. (Part 1) Isolation of microorganism and purification of the inhibitor Yi,D.H.;J.H.Seu
  18. J. Fd Hyg. Safety v.11 The antithrobotic effects of green tea catechins Yun,Y.P.;W.S.Kang;M.Y.Lee
  19. Kor. J. Pharmacogn v.26 Antithrombotic effects of some traditional plant medicines Yun-Choi,H.S.;K.S.Chung;M.H.Kim;J.H.Oh

피인용 문헌

  1. Screening of Thrombin Inhibitor and its DPPH Radical Scavenging Activity from Wild Insects. vol.18, pp.3, 2008, https://doi.org/10.5352/JLS.2008.18.3.363
  2. Antifungal Activity of Zanthoxylium schinifolium Against Fusarium graminearum, a Barley Powdery Mildew Fungus. vol.18, pp.7, 2008, https://doi.org/10.5352/JLS.2008.18.7.974
  3. Analysis of the Terpenoids from Syneilesis palmata Essential Oil and the Variation of the Sesquiterpene Compounds by Harvest Year vol.26, pp.2, 2013, https://doi.org/10.9799/ksfan.2013.26.2.287
  4. Antioxidant activities of hot water extract of Syneilesis palmata root and aerial part vol.21, pp.1, 2014, https://doi.org/10.11002/kjfp.2014.21.1.9
  5. Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa vol.18, pp.4, 2011, https://doi.org/10.11002/kjfp.2011.18.4.590
  6. Thrombin Inhibition Activity of Fructus Extract of Crataggus pinnatifida Bunge vol.17, pp.4, 2007, https://doi.org/10.5352/JLS.2007.17.4.535
  7. Quinic acid derivatives from Pimpinella brachycarpa exert anti-neuroinflammatory activity in lipopolysaccharide-induced microglia vol.23, pp.7, 2013, https://doi.org/10.1016/j.bmcl.2013.01.115
  8. Evaluation of In-vitro Anticoagulation Activity of 35 Different Seaweed Extracts vol.20, pp.11, 2010, https://doi.org/10.5352/JLS.2010.20.11.1640
  9. Comparative analysis of elemental compositions of selected edible wild plants vol.25, pp.3, 2018, https://doi.org/10.11002/kjfp.2018.25.3.330