생명과학회지 (Journal of Life Science)

  • 제14권3호
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  • Pages.472-477
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  • 2004
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Carotenogenesis의 생성 자극 인자를 이용한 Xanthophyllomyces dendrorhous에 의한 astaxanthin 산물의 증가

Improved Astaxanthin Production of Xanthophyllomyces dendrorhous with Carotenogenesis Stimulating Factors

  • Xu, Bao-Jun (The Pharmaceutical Institute, Dalian University) ;
  • Sung, Chang-Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Li, Chang-Tian (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Mo, Eun-Kyoung (Biotechnology Research Institute, Dbio Inc.,)
  • 발행 : 2004.06.01
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초록

야생 균주 Xanthophyllomyces dendrorhous으로부터 biomass와 astaxanthin 산물을 증가시키기 위해 shake flask배양 조건에서 astaxanthin산물에 영향을 미치는 물리적 인자, 영양 인자와 Carotenogenesis의 생성 자극 인자에 대한 연구를 HPLC분석 방법을 이용하여 수행하였다. 최적에 온도, 초기 pH값, 탄소와 질소 공급 조건에서 Carotenogenesis의 선구 물질인 acetic 산, mevalonic 산, 토마토 추출물과 당근 추출물은 astaxanthin 생산을 상당히 증가시킬 수 있었다.

To improve biomass and astaxanthin production by wild-type Xanthophyllomyces dendrorhous simultaneously in shake flask culture, physical factors, nutritional factors and carotenogenesis stimulating factors affecting astaxanthin production were studied on base of HPLC quantitative analysis. The results suggested that carotenogenesis precursor composition acetic acid, mevalonic acid, tomato extract, and carrot extract could increase the productivity of astaxanthin markedly based on the optimized temperature, initial pH value, carbon and nitrogen sources conditions.

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참고문헌

  1. An, G. H., C. H. Kim, E. S. Choi, and S. K. Rhee. 1996. Medium optimiza-tion of acarotenoid -hyperproducing Phaffia rhodozyma mutant HT-5FO1C. J. Ferment. Bioeng. 82, 515-518 https://doi.org/10.1016/S0922-338X(97)86996-6
  2. An, G. H., M. H. Cho, and E. A. Johnson. 1999. Monocyclic carotenoid biosynthetic pathway in the yeast Phaffia rhodozyma (Xanthophyllomyces dendrorhous). J. Biosci. Bioeng. 88, 189-193 https://doi.org/10.1016/S1389-1723(99)80200-X
  3. An, G. H. 2001. Improved growth of the red yeast Phaffia rhodozyma, in the presence of tricarboxylic acid cycle intermediates. Biotechnol. Lett. 23, 1005-1009 https://doi.org/10.1023/A:1010523800650
  4. Andrewes, A. G., H. J. Phaff, and M. P. Starr. 1976. Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting yeast. Phytochemistry 15, 1003-1007 https://doi.org/10.1016/S0031-9422(00)84390-3
  5. Calo, P., T. D. Miguel, J. B. Velzquez, and T. G. Villa. 1995. Mevalonic acid increases trans-astaxanthin and carotenoid biosynthesis in Phaffia rhodozyma. Biotechnol. Lett. 17, 575-578 https://doi.org/10.1007/BF00129380
  6. Fang, T. J., and Y. S. Cheng. 1993. Improvement of astaxanthin production by a Phaffia rhodozyma through mutation and optimization of culture conditions. J. Ferment. Bioeng. 75, 466-469 https://doi.org/10.1016/0922-338X(93)90099-T
  7. Goldstein, J. L. and M. S. Brown. 1990. Regulation of the mevalonate pathway. Nature 343, 425-430 https://doi.org/10.1038/343425a0
  8. Johnson, E. A. and G. H. An. 1991. Astaxanthin from microbial sources. CRC Critical Reviews in Biotechnology 11, 297-326 https://doi.org/10.3109/07388559109040622
  9. Johnson, E. A. and M. J. Lewis. 1979. Astaxanthin formation by the yeast Phaffia rhodozyma. J. Gen. Microbiol. 115, 173-183 https://doi.org/10.1099/00221287-115-1-173
  10. Meyer, P. S., and J. C. Du preez. 1994. Effect of culture onditions on astaxanthin production by a mutant of Phaffia rhodozyma in batch and chemostat culture. Appl. Microbiol. Biotechnol. 40, 780-785 https://doi.org/10.1007/BF00173974
  11. Miki, W. 1991. Biological functions and activities of animal carotenoids. Pure Appl. Chem. 63, 141-146 https://doi.org/10.1351/pac199163010141
  12. Naguib, Y. 2000. Antioxidant activities of astaxanthin and related carotenoids. J. Agr. Chem. 48, 1150-1154 https://doi.org/10.1021/jf991106k
  13. Parajo, J. C., V. Santos, and M. Vazquez. 1997. Production of carotenoids by Xanthophyllomyces dendrorhous growing on enzymatic hydrolysates of pre-hydrolysed wood. Food chemistry 60, 347-355 https://doi.org/10.1016/S0308-8146(96)00341-X
  14. Schroeder, W. A., and E. A. Johnson. 1993. Antioxidant role of carotenoids in Phaffia rhodozyma. J. Gen. Microbiol. 139, 907-912 https://doi.org/10.1099/00221287-139-5-907
  15. Shinidzu, N., M. Goto, and W. Miki. 1996. Carotenoids as singlet oxygen quenchers in marine organisms. Fish. Sci. 61, 134-137
  16. Spencer, K. G. 1989. Pigmentation supplements for animal feed composition. US Patent 4,871,551
  17. Xu, X. M., Z. Y. Jin, and D. H. Liu. 2000. Astaxanthin production by Phaffia rhodozyma in shake flask culture. Journal of Wuxi University of Light Industry (in Chinese) 19, 230-235