생명과학회지 (Journal of Life Science)

  • 제14권3호
  • /
  • Pages.467-471
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  • 2004
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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버섯병원균에 대한 길항세균 Bacillus sp. SD-10이 생산하는 항균물질의 분리

Isolation of Antimicrobial Substance by Produced Bacillus sp. SD-10 with Antagonistic Activity Towards Mushroom Pathogens

  • 이상원 (진주산업대학교 미생물공학과) ;
  • 류현순 (진주산업대학교 미생물공학과) ;
  • 갈상완 (진주산업대학교 미생물공학과) ;
  • 박기훈 (경상대학교 농업생명과학과) ;
  • 김철호 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학과)
  • 발행 : 2004.06.01
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초록

Bacillus sp. SD-10이 생산하는 항균물질을 이용하여 버섯의 갈색무늬병균 및 푸른곰팡이병균에 대한 천연물 버섯병해 방제약재 개발을 위한 기초적 연구를 행하였다. Bacillus sp. SD-10은 35∼4$0^{\circ}C$의 배양온도에서 30∼40 hrs 배양하였을 때 가장 높은 항균활성을 나타내었다. 이 배양액을 이용하여 T. virens의 균사 생육저해 및 포자발아 억제 농도를 검토한 결과, 균사생육은 30 ul 이상 처리 시 뚜렷하게 저해되었으며 포자발아는 평판배지에 5%(v/v) 이상 첨가 시 완전하게 억제되었다. Bacillus sp. SD-10의 배양액으로부터 항균물질을 순수분리하여 UPX-1으로 명명하고, UPX-1을 $^1H-$NMR과 $^{13}C$-NMR 스펙트럼으로 구조를 동정한 결과 탄소 6개로 구성된 당 2개가 2당류 구조로 결합되어 있는 화합물임이 밝혀졌다. UPX-1은 T. virens 뿐 아니라 Pse. tolaassi에도 아주 강한 항균효과를 나타내었다.

Bacillus sp. SD-10 was investigated to develope biological pesticides for control of mushroom diseases. Bacillus sp. SD-10 showed high antifungal activity when cultured at 35∼4$0^{\circ}C$ for 30∼4$0^{\circ}C$. The culture filtrate of the bacterium inhibited the growth of mycelium of T. virens which is a kind of mushroom pathogene. On the test of inhibition of spore germination of T. virens, more than 5% of the culture filtrate in the media inhibited completely the germination of the spores. An antimicrobial substance, UPX-1 was purified from the culture filtrate of the Bacillus. From the $^1H$-NMR and $^{13}C$-NMR spectrum analysis, the substance was indentifed as disaccharide composed to six carbon sugars. UPX-1 has not only strong antifungal activity against T. virens but also antibacterial activity against Pseudomonas tolaassi.

키워드

참고문헌

  1. J. Korean Soc. Agric. Chem. Biotechnol. v.45 Development of optimal culture media for the stable production of mushroom Gal,S.W.;S.W.Lee
  2. Proceedings Eighth Aust. Biotechnol. Conference A biological control agent for the mushroom industry Healey,K.W.;J.M.Harvey
  3. Korean J. Plant Pathol. v.10 Studies on the pathogenic Pseudomonas causing bacterial disease of cultivated mushrooms in Korea 1. On the causal organisms of the rots of Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes Kim,J.W.;K.H.Kim;H.J.Kang
  4. Korean J. Plant Pathol. v.11 Studies on the pathogenic Pseudomonas causing bacterial disease of cultivated mushrooms in Korea. 2 Bacteriological characteristics of P. tolaasi causing mushroom brown blotch and white line reacting organisms Kim,J.W.;S.I.Kwon;H.J.Kang
  5. Mushroom v.3 Mushroom diseases and protection strategies Lee,J.G.
  6. J. Am. Chem. Soc. v.113 Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii paine Nutkins,C.J.;J.R.Mortishire-Smith;C.L.Packman;L.C.Brodey;B.P.Rainey https://doi.org/10.1021/ja00007a040
  7. Ann. Appl. Biol. v.5 Studies in bacteriosis II. A brown blotch disease of cultivated mushroom Paine,S.G. https://doi.org/10.1111/j.1744-7348.1919.tb05291.x
  8. Jinju National University, M.S. Thesis Production and characteristics of bacteriocin of Bacillus sp. HS-25 isolated from traditional Doenjang and properties of its product Ryu,H.S.
  9. J. Phytopathol. v.134 Identification of antagonists for biological control of the post harvest pathogens Pezicula malicortics and Nectria galligena on apple Schiewe,A.;K.Mendgen https://doi.org/10.1111/j.1439-0434.1992.tb01231.x
  10. Mushroom v.5 Mushroom pathogen and its protection Seo,G.S.
  11. Phytopathology v.5 A bacterial disease of cultivated mushrooms Tolaas,A.G.
  12. Phytopathology v.86 Postharvest discoloration of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. reactarts and P. gingeri Wells,J.M.;G.M.Sapers;W.F.Felt;J.E.Butterfield;B.Jones;H.Bouzar;F.C.Miller https://doi.org/10.1094/Phyto-86-1098

피인용 문헌

  1. Growth Inhibition of Mushroom Pathogen by Bacillus sp. HJ 57 vol.22, pp.6, 2012, https://doi.org/10.5352/JLS.2012.22.6.799