DOI QR코드

DOI QR Code

ISSRs 마크에 의한 고려 인삼의 분자적 인증과 유전적 다형현상

Molecular Authentication and Genetic Polymorphism of Korean Ginseng (Panax ginseng C. A. Meyer) by Inter-Simple Sequence Repeats (ISSRs) Markers

  • Bang, Kyong-Hwan (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Lee, Sung-Woo (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Hyun, Dong-Yun (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Cho, Joon-Hyeong (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Cha, Seon-Woo (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Seong, Nak-Sul (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
  • 발행 : 2004.06.01

초록

ISSR마크를 사용하여 고려 인삼의 품종 및 계통간 분자적 인증과 유전적 다형현상을 조사하였다. 56개의 ISSR 프라이머 중 5개가 일곱 품종 및 계통간 명확하고 재현성이 높은 DNA분절을 나타내는 최적 프라이머로 선택되었다. 전체 43밴드는 250 bp - 1,700 bp의 분자량을 가지며 프라이머당 8.6개의 밴드를 나타내었다. 고려 인삼에서 다형현상 정도는 20.9%였다. 특히 천풍 품종이 가장 높은 다형현상을 나타낸 반면 다른 품종은 거의 다형현상을 나타내지 않았다. 결론적으로 DNA수준에서 ISSR마크로 천풍이 다른 고려 인삼의 품종 및 계통인 연풍, 황숙종, 자경종과 구분에 이용될 수 있음이 판명되었다.

Molecular authentication and genetic polymorphism of Korean ginseng cultivars and accessions were investigated using ISSR (inter-simple sequence repeat amplification) markers. Five primers among 56 produced clear and reproducible DNA fragments among seven cultivars and accessions. A total of 43 bands ranging from 250 bp to 1,700 bp from five primers were scored. Average number of bands per primer was 8.6 and only nine bands were polymorphic across the six Panax ginseng from Korea. Especially Chunpoong cultivar exhibited the highest level of polymorphism, whereas other accessions did not showed almost any polymorphism. Consequently, these ISSR markers will be available to differentiate Chunpoong cultivar from other major Korean ginseng cultivars and accessions, such as Yunpoong, Hwangsukjong and Jakyungjong, at the DNA level.

키워드

참고문헌

  1. Theor. Appl. Genet. v.98 Intersimple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.) Blair,M.W.;O.Panaud;S.R.McCouth https://doi.org/10.1007/s001220051135
  2. The Complete German Commission E. Monographs : Therapeutic Guide to Herbal Medicines (1st ed.) Blumenthal,M.
  3. Proc. Int. Ginseng Conf. Vancouver 1994 The Ginseng Plant: Products, and Quality But,P.P.H.;S.Y.Hu;H.Cao;Bailey,W.G.(ed.);C.Whitehead(ed.);J.T.A.Proctor(ed.);J.T.Kyle(ed.)
  4. Plant Mol. Biol. Rep. v.1 A plant DNA minipreparation: version II Dellaporta,S.L. https://doi.org/10.1007/BF02712670
  5. Phytochem. Bull. v.19 A rapid DNA isolation procedure for small quantities of fresh leaf tissue Doyle,J.J.;J.L.Doyle
  6. J. Am. Board Fam. Pract. v.10 Dietary supplements users; demographics, product use, and medical system interaction Eliason,B.C.;J.Kruger
  7. Ginseong Fetrow,C.W.;J.R.Avila
  8. Ginseng and Related Herbs. Tyler's Honest Herbal A sensible Guide to the Use of herbs and Related Remedies (4the ed.) Foster,S.;V.E.Tyler
  9. Biol. Pharm. Bull. v.20 Application of RCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three Ginseng drugs Fushimi,H.;K.Komatsu;M.Isobe;T.Namba https://doi.org/10.1248/bpb.20.765
  10. Planta Med. v.67 Direct amplification of length polymorphism analysis differentiates Panax ginseng from P. quinquefolius Ha,W.Y.;F.C.Yau;P.P.But.;J.Wang;P.C.Shaw https://doi.org/10.1055/s-2001-16483
  11. Mol. Genet. Genomics v.266 Isolation and characterization of repetitive DNA sequences from Panax ginseng Ho,I.S.;F.C.Leung https://doi.org/10.1007/s00438-001-0617-6
  12. Theor. Appl. Genet. v.100 Genetic diversity and relationships of sweet potato and its wild relatives in Ipomea series Batatas(Convolvulaceae) as revealed by inter-simple sequence repeat(ISSR) and restriction analysis of chloroplast DNA Huang,J.C.;M.Sun https://doi.org/10.1007/s001220051386
  13. Mol. Breed. v.1 Assessment of genetic diversity in dent and popcorn(Zea mays L.) inbreed lines using inter-simple sequence repeat(ISSR) amplification Kantety,R.V.;X.Zhang;J.L.Bennetzen;B.Z.Zehr https://doi.org/10.1007/BF01248414
  14. Genome v.38 An evaluation of sequence tagged microsatellite site markers for genetic analysis within Citrus and related species Kijas,J.M.H.;J.C.S.Fowler;M.R.Thomas https://doi.org/10.1139/g95-045
  15. Kor. J. Genetics v.25 Genetic diversity and relationship in Korean ginseng (Panax schinseng) based on RAPD analysis Kim,C.K.;H.K.Choi
  16. Theor. Appl. Genet. v.96 Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers Kojima,T.;T.Nagaoka;K.Noba;Y.Ogihara
  17. Ginseng, Panax Herbal Medicines. A Guide for Healthcare Professionals Newall,C.A.;L.A.Anderson;J.D.Phillipson
  18. Phytochemistry v.50 Molecular authentication of Panax species Ngan,F.;P.Shaw;P.But;J.Wang https://doi.org/10.1016/S0031-9422(98)00606-2
  19. The Therapeutic Use of Phytomedicinals Performance and Immune Deficiencies. Tyler's Herbs of Choice Robbert,J.E.;V.E.Tyler
  20. Genetics and Conservation of Rare Plants Comparison of methods for assessing genetic variation in plant conservation biology Schaal,B.A.;W.J.Leverich;S.H.Rogstad;Falk,D.A.(ed.);K.E.Holsinger(ed.)
  21. Planta Med. v.61 Authentication of Panax species and their adulterants by random-primed polymerase chain reaction Shaw,P.C.;P.P.But https://doi.org/10.1055/s-2006-958138
  22. Nucleic Acids Res. v.12 Simple sequences are ubiquitous repetitive components of eukaryotic genomes Tautz,D.;M.Renz https://doi.org/10.1093/nar/12.10.4127
  23. Genome v.20 Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification Zietkiewicz,E.;A.Rafalske;D.Labuda
  24. Molecular Biology v.32 Genetic variation of Wild Ginseng populations (RAPD analysis) Zhuravlev,Y.N.;G.D.Reunova;E.V.Artyukova;M.M.Kozyrenko;T.I.Muzarok

피인용 문헌

  1. Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes vol.35, pp.4, 2011, https://doi.org/10.5142/jgr.2011.35.4.504
  2. A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar “Chunpoong” vol.37, pp.2, 2010, https://doi.org/10.1007/s11033-009-9827-5
  3. Molecular authentication of Panax ginseng and ginseng products using robust SNP markers in ribosomal external transcribed spacer region vol.55, pp.5, 2011, https://doi.org/10.1016/j.jpba.2011.03.037
  4. Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences vol.1, pp.3, 2007, https://doi.org/10.1007/s11816-007-0019-2
  5. A Nucleotide Signature for the Identification of American Ginseng and Its Products vol.7, 2016, https://doi.org/10.3389/fpls.2016.00319
  6. Development of Molecular Markers for the Determination of the New Cultivar ‘Chunpoong’ in Panax ginseng C. A. MEYER Associated with a Major Latex-Like Protein Gene vol.33, pp.2, 2010, https://doi.org/10.1248/bpb.33.183
  7. A simple and rapid technique for the authentication of the ginseng cultivar, Yunpoong, using an SNP marker in a large sample of ginseng leaves vol.487, pp.1, 2011, https://doi.org/10.1016/j.gene.2011.05.021
  8. A plant-transformation-competent BIBAC library of ginseng (Panax ginseng C. A. Meyer) for functional genomics research and characterization of genes involved in ginsenoside biosynthesis vol.31, pp.3, 2013, https://doi.org/10.1007/s11032-012-9826-4
  9. Molecular identification of the Korean ginseng cultivar “Chunpoong” using the mitochondrialnad7intron 4 region vol.20, pp.2-3, 2009, https://doi.org/10.1080/19401730902856738
  10. Ethnopharmacological Investigation and Rapid Authentication of Mongolian Patent Medicines Digeda vol.7, pp.3, 2015, https://doi.org/10.1016/S1674-6384(15)60044-2
  11. Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species vol.21, pp.2, 2013, https://doi.org/10.7783/KJMCS.2013.21.2.91
  12. The Spatial and Temporal Transcriptomic Landscapes of Ginseng, Panax ginseng C. A. Meyer vol.5, pp.1, 2016, https://doi.org/10.1038/srep18283
  13. CAPS markers using mitochondrial consensus primers for molecular identification of Panax species and Korean ginseng cultivars (Panax ginseng C. A. Meyer) vol.39, pp.1, 2012, https://doi.org/10.1007/s11033-011-0792-4