Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Lectin Histochemistry of the Glycoconjugates in the Esophageal Mucous Cells of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis

조피볼락, 용치놀래기, 송곳니베도라치 및 졸복 식도 점액세포의 복합당질에 대한 Lectin 조직화학

  • 정길남 (부산대학교 사범대학 생물교육과) ;
  • 이응희 (부산대학교 사범대학 생물교육과) ;
  • 조기진 (부산대학교 사범대학 생물교육과) ;
  • 정권순 (부산대학교 사범대학 생물교육과) ;
  • 조운복 (부산대학교 사범대학 생물교육과)
  • Published : 2004.06.01
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Abstract

This study attempts to investigate lectin binding patterns of the glycoconjugates of the esophageal mucous cells in four teleostean speceis, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis. To investigate glycoconjugates of esophageal mucous cells, nine biotinylated lectins (DBA, SBA, PNA, BSL-l, RCA-l, sWGA, UEA-l, LCA and ConA) were applied with ABC method. Esophageal mucous cells of Sebastes schlegeli and Halichoeres poecilopterus were mixed with large, medium sized and small mucous cells. But these cells of the other species only were mixed with medium sized and small mucous cells. The lectin binding pattern of esophageal mucous cells depends on the species; Sebastes schlegeli was stained with DBA, SBA, BSL-l, RCA-l and sWGA, Halichoeres poecilopterus with DBA, SBA, PNA and sWGA, Bryzoichthys lysimus with SBA and sWGA, Takifugu pardalis with all lectins except DBA, LCA and Con A, respectively. All the mucous cells of Sebastes schlegeli were stained with DBA, SBA and sWGA, while small mucous cells with BSL-l besides these lectins. In Halichoeres poecilopterus,l the large mucous cells reacted with PNA, medium sized mucous cells with DBA, SBA and sWGA, and small mucous cells with DBA and SBA, respectively. Medium sized mucous cells of Bryzoichthys lysimus were stained with sWGA, and small mucous cells with SBA and sWGA. In Takifugu pardalis, all mucous cells reacted with SBA, PNA and RCA-l, but medium sized mucous cells with sWGA and UEA-l besides these lectins. Especially DBA and SBA lectins showed a strong binding to all mucous cells of Sebastes schlegeli. In Halichoeres poecilopterus, PNA binding were notable in large mucous cells, and SBA binding in medium sized and small cells, respectively. However, SBA, PNA, sWGA and UEA-l lectins of Takifugu pardalis showed a strong binding to medium sized mucous cells, but RCA-l binding which small mucous cells were notable.

경골어류인 조피볼락, 용치놀래기, 송곳니베도라치 및 졸복 식도 점액세포의 복합당질 성상을 biotinylated lectin인 DBA, SBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, LCA 및 Con A로 연구하였다. 조피볼락과 용치놀래기는 큰ㆍ중간 및 작은 점액세포가 섞여 있었고, 송곳니베도라치와 졸복은 중간 및 작은 점액세포들이 섞여 있었다. 식도 점액세포들의 lectin 결합양상도 어종에 따라 차이가 있었으며 조피볼락은 DBA, SBA, BSL-1, RCA-1 및 sWGA에, 용치 놀래기는 DBA, SBA, PNA 및 sWGA에, 송곳니베도라치는 SBA 및 sWGA에 각각 반응을 하였으며 졸복은 DBA, LCA 및 Con A를 제외한 모든 lectin에 반응하였다. 조피볼락의 모든 점액세포에는 DBA, SBA 및 sWGA가, 작은 점액세포에는 이 외에 BSL-1이 반응하였다. 용치놀래기의 큰 점액세포에서는 PNA가, 중간 점액세포에는 DBA, SBA 및 sWGA가, 작은 점액세포에는 DBA와 SBA가 반응하였다. 송곳니베도라치의 중간 점액세포에는 sWGA가, 작은 점액세포에는 SBA와 sWGA가 반응하였으며 졸복은 모든 점액세포에 SBA, PNA 및 RCA-1가, 중간 점액세포에는 이 외에 sWGA와 UEA-1이 반응하였다. 특히 조피볼락의 점액세포에서 DBA와 SBA의 양이 많고, 용치놀래기에서는 큰 점액세포에서 PNA 양이, 중간 및 작은 점액세포에서 SBA양이 많았으며, 졸복의 경우 중간 점액세포에서 SBA, PNA, sWGA 및 UEA-1의 양이, 작은 점액세포에서 RCA-1의 양이 많았다.

Keywords

References

  1. Textbook of comparative histology Andrew,W.
  2. Histology of the vertebrates Andrew,W.;C.P.Hickman
  3. J. Fish Biol. v.3 The anatomy and histology of the alimentary tract of the carnivorus fish, the pike Esoxlucius Bucke,D. https://doi.org/10.1111/j.1095-8649.1971.tb05914.x
  4. J. Morph. v.65 The histology of the digestive tube of the carp(Cyprines carpio commuins) Curry,E. https://doi.org/10.1002/jmor.1050650104
  5. J. Cell Biol. v.62 Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectin Etzler,M.F.;M.L.Branstrator https://doi.org/10.1083/jcb.62.2.329
  6. Adv. Carbohydr. Cytochem. Biochem. v.35 The lectins: carbohydrate-binding proteins of plant and animal Goldstein,I,J.;C.E.Hayes https://doi.org/10.1016/S0065-2318(08)60220-6
  7. Biochem. Soc. Trans. v.1 Epithelial mucins of the atlantic salmon (Salmon salar L.) Harris,J.;S.Hunt
  8. Histochemistry v.88 Light microscopic characterization of glycoconjugates in secretory cells of the carp (Cyprinus carpio) gill epithelium Hidalgo,J.;A.Velasco;I.Sanchez Aguayo;P.Amores https://doi.org/10.1007/BF00490170
  9. Cancer v.47 Carcinoma associated cytostructural antigenic alteration; Detected by lectin binding Howard,D.R.;P.Ferguson;J.G.Batsicis https://doi.org/10.1002/1097-0142(19810615)47:12<2872::AID-CNCR2820471220>3.0.CO;2-S
  10. Epithelial transport in lower vertebrates Functional morphology of fish gills Hughes,G.M.;Lahlon,B.(ed.)
  11. Alfred Benzon Symposium 11, Osmotic and Volume regulation External charged layer and $Na^{+}$ regulation Kirschner,L.B.
  12. J. Histochem. Cytochem. v.13 On the use of testicular hyaluronidase for identifying acid mucins in tissue sections Leppi,T.J.;R.J.Stoward https://doi.org/10.1177/13.5.406
  13. Acta. Histochem. Cytochem. v.22 no.1 Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry Madrid,J.F.;J.Bellesta;M.T.Castells;J.A.Marin https://doi.org/10.1267/ahc.22.1
  14. Stain Techn. v.23 (AFIP modification) Periodic acid Schiff's reaction McManus,J.F.A.
  15. Biochem. Physiol. v.72 Distribution of mucosal macromolecular glycoprotein in rat stomach, Comp. Ohara,S.;K.Ischihara;M.Kakei;Y.Azuumi;K.Hotta https://doi.org/10.1016/0305-0491(82)90051-7
  16. Comparative vertebrate histology Patt,D.I.;G.R.Patt
  17. Dev. Brain Oes. v.68 Expression of lectin binding in the superficial dorsal horn of the rat spinal-cord during prenatal and postnatal-development Plenderleith,M.B.;L.L.Wright;D.J.Snow https://doi.org/10.1016/0165-3806(92)90252-R
  18. J. Morph v.152 Structure and carbohydrate histochemistry of the esophagus in ten teleostean species Reifel,C.W.;A.A.Travill https://doi.org/10.1002/jmor.1051520303
  19. Anat. Anz. v.144 Gross morphology of the alimentary canal in ten teleostean species Reifel,C.W.;A.A.Travill
  20. I. Mouse Histochem. J. v.15 Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates Schulte,B.A.;S.S.Spicer https://doi.org/10.1007/BF01002741
  21. J. Histochem. Cytochem. v.31 Light microscopic histochemical detection of terminal galactose and N-ace-tylgalactosamine residues in rodent complex carbohydrates using a galactose-oxidase-Schiff sequence and peanut lectin-horseradish peroxidase conjugate Schulte,B.A.;S.S.Spicer https://doi.org/10.1177/31.1.6187799
  22. J. Histochem. Chtochem. v.31 Light microscopic histochemical detection of sugar residues in secretory glycoproteins of rodent and human tracheal glands with lectin-horseradish peroxidase conjugates and the galactose oxidase-Schiff sequence Schulte,B.A.;S.S.Spicer https://doi.org/10.1177/31.3.6186733
  23. J. Histochem. Cytochem. v.33 no.5 Histochemical methods for characterizing secretory and cell surface sialo-glycoconjugates Schulte,B.A.;S.S.Spicer https://doi.org/10.1177/33.5.3989272
  24. Science v.177 Lectins as cell recognition Sharon,N.;H.Lis https://doi.org/10.1126/science.177.4053.949
  25. Connective tissue disease Cytochemistry of complex carbohydrates by light and electron microscopy : Available methods and their application Spicer,S.S.;B.A.Schulte;G.N.Thomopoulos;R.T.Parmley;M.Takagi;Wagner,B.A.(ed.)
  26. Histochemistry v.73 Post-embedding staining of rat gastric mucous cells with lectins Suzuki,S.;S.Tsuyama;F.Murata https://doi.org/10.1007/BF00493369
  27. Histochemistry v.86 Evaluation on secretory glycoconjugates in normal human jejunum by means of lectin histochemistry Vecchi,M.;G.Torgano;M.Monti;E.Berti;D.Agape;M.Primignani;G.Ronchi;R.Franchis https://doi.org/10.1007/BF00494993
  28. J. Histochem. Cytochem. v.29 Discrete distribution of binding sites for Dolichos biflorus agglutinin(DBA) and for peanut agglutinin(PNA) in mouse organ tissue Watanabe,M.;T.Muramatsu;H.Shirane;K.Ugai https://doi.org/10.1177/29.7.7053086
  29. J. Histochem. v.24 Changes in lectin binding pattern of leydig-cells during fetal and postnatal development in mice Zhou,X.H.;H.Kawakami;H.Hirano https://doi.org/10.1007/BF01046167
  30. J. Pusan Med. Coll. v.35 no.1 Differential glycoconjugate expression in the goblet cells of the rat proximal colon Kim,K.L.;S.C.Moon;S.Y.Baek;J.B.Kim;B.S.Kim
  31. J. Sci. Pusan Natl. Uni. v.14 Comparative histochemical study on the mucosa of the digestive tract of fresh water fishes Kim,Y.K.
  32. J. Catholic Med. Coll. v.36 no.4 Histochemical study on mucosubstances of the mucous cells in teleostean esophagus Im,M.S.;H.S.Kwun
  33. J. Mol. Bio. Pusan Natl. Uni. v.1 Histochemical study on mucosubstances of the esophageal mucous cells in the teleosts Yi,S.H.;U.B.Jo
  34. Korean J. Life Sci. v.10 no.2 Histochemical study on glycosaminoglycans of esophageal mucous cells in Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma and Helicolenus dactylopterus Jung,K.S.;M.S.Hong;G.J.Jo;U.B.Jo
  35. J. Pusan Natl. Uni. v.13 Comparative study on the digestive mucosa of frogs and fishes Jo,U.B.;H.C.Park
  36. Korean J. Anat. v.7 no.1 Histochemical studies on the acid mucosubstances in the mucosa of alimentary tract of lower vertebrates Jo,U.B.
  37. J. Sci. Pusan Natl. Uni. v.35 Histochemical study of the properties of mucosubstances on the esophageal mucous cells in five teleostean species Jo,U.B.;I.J.Choi.