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서실의 항산화, 항균효과 연구

Studies on the Antioxidative and Antimicrobial Effects of Chondria crassicaulis

  • 배송자 (신라대학교 식품영양학과, 마린바이오 산업화 지원센터)
  • 발행 : 2004.06.01

초록

홍조류의 하나인 서실을 추출, 분획하여 각 용매별 항산화와 항균효과를 관찰하였다. 서실의 항산화 효과를 측정하기 위해 DPPH radical 소거능과, $.$ $O^{2-}$$.$ scavenging activity를 측정함으로서 $.$ $O^{2-}$ 제거능을 실험하였다. 항산화 효과를 실험한 결과는 메탄을 분획층인 CCMM층이 대조군으로 사용한 일반적 항산화제인 BHT와 vitC와 유사한 DPPH radical 소거능을 보였고, $O^{2-}$ 제거능은 CCMM층과 CCMA층에서 활성을 보였다. 본 실험의 결과로 CCMM층에서 항산화 효과가 제일 좋았고 CCMA층에서도 항산화 효과를 가지는 물질이 있을 것으로 사료된다. paper disc method를 이용한 항균활성 효과 실험 결과, 홍조 해조류인 서실의 Bacillus subtilis를 제외하고는 CCMB층에서의 항균력이 가장 높게 나타났으며 실험 군주 4종 모두 CCMH층에서는 미약하나마 효과가 있었다. 본 실험 결과 홍조 해조류인 서실의 항산화 효과 검색에서 Methanol층인 CCMM층이 항산화 효과가 가장 좋았으며 항균효과 검색에서는 CCMB층이 활성을 나타내었다. 이 연구결과를 토대로 서실의 항산화 및 항균활성물질에 대찬 단계적인 분리 동정이 이루어져 CCMM층과 CCMB층의 생리활성물질 개발이 기대되는 바이다.

In this study, we investigated the biological activity of antioxidant and antimicrobiological effect of Chondria crassicaulis (CC), which, using methanol, dichlolometane and ethanol, were extracted and fractionated into four different types: hexane(CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. The reducing activity on the 1,I-diphenyl-2-picrylhydrazyl (DPPH) radical and $O^{2-}$ and $O_{2}$$O_{2}$radical scavenging potential, in search for antioxidation effects of CC partition layer, were sequentially screened. Among the four fractions, CCMM had the highest antioxidative activity. The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the various solvent layers, the CCMB, CCMH and CCMM showed relatively strong antimicrobial activities in the order.

키워드

참고문헌

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피인용 문헌

  1. Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts vol.21, pp.4, 2011, https://doi.org/10.5352/JLS.2011.21.4.576
  2. Anti-Inflammatory Effect of Chondrus nipponicus Yendo Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells vol.45, pp.2, 2016, https://doi.org/10.3746/jkfn.2016.45.2.194
  3. The Antioxidative and Antimicrobial Effects of Internal Organs of Aplysia kurodai Fractions vol.39, pp.10, 2010, https://doi.org/10.3746/jkfn.2010.39.10.1433
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  5. Anti-Inflammatory Effect of Chondria crassicaulis Ethanol Extract on MAPKs and NF-κB Signaling Pathway in LPS-Induced RAW 264.7 Macrophages vol.32, pp.4, 2017, https://doi.org/10.7841/ksbbj.2017.32.4.352