Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Chitosan on Fracture Healing in Fractured Rat Model.

골절모델 쥐에서 키토산 투여가 골절치유에 미치는 영향

  • 서정욱 (한국화학연구원 부설 안전성평가연구소) ;
  • 김은주 (한국화학연구원 부설 안전성평가연구소) ;
  • 한상섭 (한국화학연구원 부설 안전성평가연구소)
  • Published : 2004.06.01
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Abstract

Chitosan is abundant polysaccharide polymer in nature Chitosan has been the subject of much research regarding its potential as a nutritional adjunct and pharmaceutical ingredient. In the present study, we examined fracture-healing process with chitosan administration and low calcium diet in rats. Left femur was fractured and fixed with intramedullary pin. The rats were fed normal diet or low calcium diet and administered chitosan with the doses of 0, 50, 100 and 150ng/ml orally 5 times a week for 10 weeks. Soft X-ray and mechanical testing of all fractured femora was taken. Radiographical finding showed that the callus formation and process of fracture healing was present in all the fractured femora. Mechanical testing indicated that the maximum load and stiffness of femur in rat fed low calcium diet was lower than those of that in rat fed normal diet. No difference in maximum load and stiffness of fractured femora in chitosan treated rat were observed as compared to vehicle treated rat. Chitosan or/and low calcium diet did not affect the ratio of fractured/unfractured femur about maximum load and stiffness. The results suggest that chitosan dose not affect the bone mechanical strength and the process of fracture healing. Low calcium diet does decrease the bone mechanical strength.

키토산은 자연에 풍부하게 존재하는 다당의 중합체로 식품이나 약제의 성분으로 활용성이 있어 많은 연구가 이루어져 왔다. 본 연구에서는 키토산 투여와 저칼슘사료 급이가 랫드에서 골절의 치유과정에 미치는 영향을 실험하였다. 좌측 대퇴골을 골절시킨 후 핀을 골내에 삽입하여 고정하였다. 이후 랫드에 일반사료 또는 저칼슘사료를 급이하고 키토산을 0, 50, 100, 150 mg/kg 용량으로 10주간 주 5회 경구투여 하였다. 실험종료 시에 골절시킨 대퇴골의 X-ray 촬영과 물리적 측정을 실시하였다. X-ray필름 관찰에서는 골절을 유발한 모든 대퇴골에서 골절치유의 과정과 가골이 형성되었다. 물리적인 골강도 측정에서는 저칼슘사료를 급이한 랫드에서 일반사료를 급이한 랫드에 비해 최대하중과 강도의 감소를 나타내었다. 키토산을 투여한 랫드와 부형제를 투여한 랫드에서의 대퇴골의 최대하중과 강도의 차이는 없었다 또한 키토산 투여 및 저칼슘사료 급이는 최대하중과 강도에 대한 골절유발 대퇴골/비유발 대퇴골의 비율에는 영향을 미치지 않았다. 이 같은 결과로 보아 키토산은 골절의 치유과정과 제의 물리적 강도에 영향을 미치지 않는 것으로 사료된다. 또한 저칼슘사료 급이는 뼈의 물리적 강도를 감소시키는 것으로 사료된다.

Keywords

References

  1. The laboratory rat. (1st eds.) Baker,H.J.;Lindsey,J.R.;Weisbroth,S.H.
  2. J. Orthop. Res. v.2 Production of a standard closed fracture in Iaboratory animal bone Bonnarens,F.;Einhorn,T.A. https://doi.org/10.1002/jor.1100020115
  3. J. Appl. Pharmacol. v.9 Therapeutic effects of safflower seed extract and chitosan on hard tissue regeneration Choung,S.Y.;Park,J.B.;Park,K.K.;Kwon,Y.H.;Kim,S.J.
  4. Drug Dev. Ind. Pharm. v.24 Chitosan: a unique polysaccharide for drug delivery Felt,O.;Buri,P.;Gurny,R. https://doi.org/10.3109/03639049809089942
  5. Lipids v.26 Effect of chitosan feeding on intestinal bile acid metabolism in rats Fukada,Y.;Kimura,K.;Ayaki,Y. https://doi.org/10.1007/BF02537206
  6. J. Orthop. Res. v.3 Effect of EHDP on fracture healing in dogs Lenehan,T.M.;Balligand,M.;Nunamaker,D.M.;Wood,F.E.Jr. https://doi.org/10.1002/jor.1100030413
  7. J. Bone Miner. Res. v.16 Long-time effect of incadronate disodium (YM-175) on fracture healing of femoral shaft in growing rats Li,C.;Mori,S.;Li,J.;Kaji,Y.;Akiyama,T.;Kawanishi,J.;Norimatsu,H.J. https://doi.org/10.1359/jbmr.2001.16.3.429
  8. J. Bone Miner. Res. v.14 Effect of bisphosphonate (incadronate) on fracture healing of long bones in rats Li,J.;Mori,S.;Kaji,Y.;Mashiba,T.;Kawanishi,J.;Norimatsu,H. https://doi.org/10.1359/jbmr.1999.14.6.969
  9. J. Bone Joint Surg. v.60B The biology of fracture healing in long bones McKibbin,B.
  10. J. Controlled Release v.46 Hydrogel beads based on amidated pectins for colon-specific drug delivery: the role of chitosan in modifying drug release Munjeri,O.;Collett,J.H.;Fell,J.T. https://doi.org/10.1016/S0168-3659(96)01607-0
  11. Carbohydr. Polym. v.36 Osteogenesis promoted by calcium phosphate N, N-diarb-oxymethyl chitosan Muzzarelli,R.A.A.;Romos,V.;Stanic,V.;Dubini,B.;Mattioli-Belmonte,M.;Tosi,G.;Giardino,R. https://doi.org/10.1016/S0144-8617(98)00008-3
  12. Biomaterials v.14 Osteoconductive properties of methylpyrrolidinone chitosan in an animal model Muzzarelli,R.A.A.;Zucchini,C.;Ilari,P.;Pugnaloni,A.;Mattioli-Belmonte,M.;Biagini,G.;Castaldini,C. https://doi.org/10.1016/0142-9612(93)90134-N
  13. Atherosclerosis v.138 Dietary chitosan inhibits hypercholesterolaemia and atherogenesis in the apoliporotein E-deficient mouse model of atherosclerosis Ormord,D.J.;Holmes,C.C.;Miller,T.E. https://doi.org/10.1016/S0021-9150(98)00045-8
  14. J. Bone Joint Surg. Am. v.85A Effect of intermittent pneumatic soft-tissue compression on fracture-healing in an animal model Park,S.H.;Silva,M.
  15. Lipids v.23 Hypocholesterolemic action of chitosans with different viscosity in rats Sugano,M.;Watanabe,S.;Kishi,A.;Izume,M.;Ohtakara,A. https://doi.org/10.1007/BF02535456
  16. Advanced Drug Delivery Reviews v.52 Oraldrug absorption enhancement by chitosan and its derivatives Thanou,M.;Verhoef,J.C.;Junginger,H.E. https://doi.org/10.1016/S0169-409X(01)00231-9
  17. Methods Find Exp. Clin. Pharmacol. v.21 Decrease in serum LDL cholesterol with microcrystalline chitosan Wuolijoki,E.;Hirvela,T.;Ylitalo,P. https://doi.org/10.1358/mf.1999.21.5.793477
  18. J. Nuri. Sci. Vitaminol. v.48 Effects of habitual chitosan intake on bone mass, bone-related matabolic markers and duodenum CaBP D9K mRNA in ovariectomized SHRSP rats Yang,C.Y.;Oh,T.W.;Nakajima,D.;Maeda,A.;Nka,T.;Kim,C.S.;Igawa,S.;Ohta,F. https://doi.org/10.3177/jnsv.48.371
  19. J. Bone Miner. Res. v.16 Low-intensity pulsed ultrasound accelerates rat femoral fracture healing by acting on the various cellular reactions in the fracture callus Yoshiaki,A.;Masaya,I.;Yoshifumi,H.;Hideko,T.;Tomohiro,O.;Seiya,J. https://doi.org/10.1359/jbmr.2001.16.4.671