초록
감잎 4품종과 녹차의 화학성분을 분석하고 항산화 활성을 비교하여 전통 건강차로써 감잎차의 우수성을 알리는 기초 자료로 활용하고자 건시료의 유리당, 유리아미노산, 비타민C, 카페인 등을 분석하였다. 감잎 4품종 모두 녹차에 비해 총 유리당 함량이 높았고 유리당은 sucrose, glucose, fructose, maltose, xylose 5종의 유리당이 동정되었으며 그 중 sucrose의 함량이 가장 높았다. 유리아미노산 함량은 감잎 품종에서 총 31∼32종이 검출되었으며, 총 유리아미노산 함량은 상주둥시 60.40 nmol/$\mu$L, 봉옥 53.21 nmol/$\mu$L, 월하시 52.29 nmol/$\mu$L, 청도반시 47.58 nmol/$\mu$L이었고 감잎에 비해 녹차가 114.72 nmol/$\mu$L로 가장 많았다. 비타민 C 함량은 월하시 0.089%, 청도반시 0.079%, 봉옥 0.032%, 상주둥시 0.015%, 녹차 0.01%로 감잎이 녹차에 비해 8배 이상 높았다. 카페인은 감잎 4품종에서는 발견되지 않았고, 녹차는 6.63 mg/100 g의 함량을 보였고, 카테친은 청도반시 0.35%, 봉옥 0.34%로 가장 높은 함량을 보였고, 녹차는 0.07%로 감잎의 1/5 정도 수준이었다. gallic acid는 감잎 품종 중에는 상주둥시 0.32%, 봉옥 0.20%가 높은 편이었고 녹차 1.41%로 가장 높았다. Ca의 함량은 청도반시가 9516.17 ppm으로 가장 높았고 녹차 2349.71 ppm로 감잎이 녹차에 비해 4∼5배정도 높았다. 향기성분 패턴은 감잎 품종간에는 비슷한 경향이었고 녹차와는 차이가 있었으며 항산화 활성은 감잎 품종 중에는 월하시가 IC50($\mu$g)가 42.0으로 가장 높게 나타났고 녹차가 IC50($\mu$g)가 19.0로 감잎에 비해 높았다.
As a foundational study for notifing excellence of persimmon leaves tea, the chemical component and antioxidant activity were investigated in persimmon leaves from Dungsi, Gabjubaekmok, Weulhasi and Cheongdobansi and green tea leaves. Total sugar contents in all persimmon leaves more higher than that of green tea leaves, and the highest free sugar contained in persimmon and green tea leaves was sucrose. Free sugars present in persimmon and green tea leaves were composed of sucrose, glucose, fructose, maltose and xylose. Sucrose and fructose took more than 70% of total sugar contents. 31∼32 kinds of amino acid were detected in persimmon leaves and 35 kinds in green tea leaves. And total amino acids contained in persimmon leaves were Dungsi, Gabjubaekmok, Weulhasi and Cheongdobansi, respectively 60.40 nmol/${\mu}$L, 53.21 nmol/${\mu}$L, 52.29 nmol/${\mu}$L and 47.58 nmol/${\mu}$L. Total amino acid contents in green tea leaves was the most abundant of all as 114.72 nmol/${\mu}$L. The contents of vitamin C in persimmon and green tea leaves were in the range of 0.015∼0.089% and 0.01%, respectively. Vitamin C was significant higher content in the persimmon leaves than in green tea leaves. Caffeine was not detected in all persimmon leaves, but the caffeine content of green tea leaves was 6.63 mg/l00 g. The content of catechin was showed in the orders of Cheongdobansi, Gabjubaekmok, Weulhasi, Dungsi and green tea leaves; 0.35%, 0.34%, 0.24%, 0.18% and 0.07%, respectively. The contents of gallic acid in Dungsi and Gabjubaekmok were 0.32% and 0.20%. That of green tea was 1.41%, it was the highest content in all samples. The content of calcium in Chengdobansi was most abundant in all samples as 3516.14 ppm, it was 4∼5 times as that of green tea leaves. Flavor component pattern among persimmon leaves was similar, but that of green tea leaves was different. The IC50(${\mu}$g) value of Dungsi, Weulhasi, Gabjubaekmok, Cheongdobansi and green tea were 64.5, 42.0, 47.0, 64.0 and 19.0 respectively.
