Application of an Interferometric Biosensor Chip to Biomonitoring an Endocrine Disruptor

  • Kim, Byung-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Lim, Sung-Hyuk (Department of Chemical Engineering, Sungkyunkwan University)
  • 발행 : 2004.03.01

초록

Recombinant E.coli ACV 1003 (recA::lacZ) releasing ${\beta}$-galactosidase by a SOS regulon system, when exposed to DNA-damaging compounds, have been used to effectively monitor endocrine disruptors. Low enzyme activity of less than 10 units/mL, corresponding to a $\mu\textrm{g}$/L(ppb) range of an endocrine disruptor (tributyl tin, bisphenol A. etc.), can be rapidly determined, not by a conventional time-consuming and tedious enzyme assay, but by an alternative interferometric biosensor. Heavily boron-doped porous silicon for application as an interferometer, was fabricated by etching to form a Fabry-Perot fringe pattern, which caused a change in the refractive index of the medium including ${\beta}$-galactosidase. In order to enhance the immobilization of the porous silicon surface, a calyx crown derivative (ProLinker A) was applied, instead of a conventional biomolecular affinity method using biotin. This resulted in a denser linked formation. The change in the effective optical thickness versus ${\beta}$-galactosidase activity, showed a linear increase up to a concentration of 150 unit ${\beta}$-galactosidase/mL, unlike the sigmoidal increase pattern observed with the biotin.

키워드

참고문헌

  1. Environ. Sci. Technol. v.32 Widespread sexual disruption in wild fish Jobling,S.;M.Nolan;C.R.Tyler;G.Brighty;J.P.Sumpter https://doi.org/10.1021/es9710870
  2. Environmental Biotechnology: Principles and Applications Luminescent biosensors for the detection of tributyltin and dimethyl sulfoxide and the elucidation of their mechanisms of toxicity Briscoe,S.F.;C.Diorio;M.S.DuBow
  3. Environmental Biotechnology: Principles and Applications Luminescent biosensors for the detection of tributyltin and dimethyl sulfoxide and the elucidation of their mechanisms of toxicity Briscoe,S.F.;C.Diorio;M.S.DuBow;M.Moo-Young(ed.)
  4. Biotechnol. Bioprocess Eng. v.5 Environmental biosensors for organochlorines, cyanobacterial toxins, and endocrine disrupting chemicals Sadik,O.A.;M.M.Ngundi;F.Yan https://doi.org/10.1007/BF02931939
  5. Microbiol. Rev. v.48 Mutagenesis and inducible responses to deoxyribonucleic acid damage in E. coli Walker,G.C.
  6. Appl. Environ. Microbiol. v.63 Detection of DNA damage by use of E. coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids Vollmer,A.C.;S.Belkin;D.R.Smulski;T.K.Van Dyk;R.A.LaRossa
  7. Gene v.103 SOS induction as an in vivo assay of enzyme-DNA interactions Heitman,J.;P.Model https://doi.org/10.1016/0378-1119(91)90383-M
  8. Optical Biosensors, Present and Future Interferometric biosensors Campbell,D.P.;C.J.McCloskey;F.S.Ligler(ed.);C.A.Rowe Taitt(ed.)
  9. Biotechnol. Bioprocess Eng. v.7 Determination of tyrosinase mRNA in melanoma by reverse transcription-PCR and optical mirror resonance biosensor Choe,T.;I.Park;S.Hong https://doi.org/10.1007/BF02932972
  10. Science v.262 Attempts to mimic docking processes of the immune system Muller,W.;H.Ringsdorf;E.Rump;G.Wildburg;X.Zhang;L.Angmaier;W.Knoll;M.Lilet;J.Spinke https://doi.org/10.1126/science.8259513
  11. Nature v.411 Microarrays of cells expressing defined cDNAs Zizuddin,M.;D.M.Sabatini https://doi.org/10.1038/35075114
  12. Angew. Chem. Int. Ed. v.39 Integration of layered redox proteins and conductive supports for bioelectronic applications Willner,I.;E.Katz https://doi.org/10.1002/(SICI)1521-3773(20000403)39:7<1180::AID-ANIE1180>3.0.CO;2-E
  13. Kor. J. Biotechnol. Bioeng. v.17 Applications of self assembled monolayers [SAMs] for biosensor Kim,E.R.
  14. Trends in Anal. Chem. v.19 Chip-based Micro-systems for genomic and proteomic analysis Sandres,G.H.S.;A.Manz https://doi.org/10.1016/S0165-9936(00)00011-X
  15. Biomaterials v.19 Protein patterning Blawas,A.S.;W.M.Reichert https://doi.org/10.1016/S0142-9612(97)00218-4
  16. Biotechnol. Prog. v.12 Development of a technique for microimmobilization of proteins on silicon wafers by a streptavidinbiotin reaction Koyano,T.;M.Saito;Y.Miyamoto;K.Kaifu;M.Kato https://doi.org/10.1021/bp9500573
  17. J. Am. Chem. Soc. v.120 Macroporous p-type silicon fabry-fringe layers: Fabrication, characterization, and application in biosensing Janshoff,A.;K.P.S.Dancil;C.Steinem;D.P.Greiner;V.S.Y.Lin;C.Gurtner;K.Motesharei;M.J.Sailor;M.R.Ghardiri https://doi.org/10.1021/ja9826237
  18. Science v.278 A porous silicon-based optical interferometric biosensor Lin,V.S.Y.;K.Motesharei;K.P.S.Dancil;M.J.Sailor;M.R.Ghardiri https://doi.org/10.1126/science.278.5339.840
  19. Proteomics v.3 ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies Lee,Y.;E.K.Lee;Y.W.Cho;T.Matsui;I.C.Kang;T.S.Kim;M.H.Han https://doi.org/10.1074/mcp.R300012-MCP200
  20. Proc. Natl. Acad. Sci. USA v.92 Overexpression of RNAase H partially complements the growth defect of an E. coli ΔtopA mutant Drolet,M.;P.Phoenix;R.Menael;E.Masse;L.F.Liu;R.J.Crouch
  21. Proc. Natl. Acad. Sci. USA v.77 DNA-damaging agents stimulates gene expression at specific loci in E. coli Kenyon,C.J.;G.C.Walker https://doi.org/10.1073/pnas.77.5.2819
  22. J. Bacteriol. v.153 Isolation and characterization of Tn5 insertion mutations in the lexA gene of E. coli Krueger,J.H.;S.J.Elledge;G.Walker
  23. Experiments in Molecular Genetics Miller,J.H.
  24. Appl. Phys. Lett. v.37 Effect of Si dissolution and recrystalization upon ohmic Al/p-Si contacts Dascalu,D.;G.H.Brezeanu;P.A.Dan https://doi.org/10.1063/1.91830
  25. J. Am. Chem. Soc. v.121 A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface Dancil,K.P.S.;D.P.Greiner;M.J.Sailor https://doi.org/10.1021/ja991421n
  26. Bioprocess Biosyst. Eng v.24 Bioassay of environmental endocrine disruptors TBT and DMSO using recombinant E. coli Cha,J.M.;B.W.Kim https://doi.org/10.1007/s004490100269
  27. Colloids and Surfaces A: Physicochem. Eng. Aspects v.207 Thermal stability of mercaptan terminated self-assembled multilayer films on SiO2 surface Senkevich,J.J.;G.R.Yang;T.M.Lu https://doi.org/10.1016/S0927-7757(02)00074-2
  28. Protein Data Bank
  29. Optics(4th ed.) Hecht,E.
  30. J. Colloid Interface Sci. v.196 Studies on the biotin-avidin multilayer adsorption by spectroscopic ellipsometry Spaeth,K.;A.Brecht;G.Gauglitz https://doi.org/10.1006/jcis.1997.5200