Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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An Immobilized Culture of Choristoneura fumiferana Cells for High Production of CfMNPV

  • Son, Jeong-Hwa (Department of Biotechnology ＆ Bioengineering, Pukyoon National University) ;
  • Buchholz, Rainer (Department of Bioprecess Engineering, Friedrich-Alexander University) ;
  • Kim, Sung-Koo (Department of Biotechnology ＆ Bioengineering, Pukyoon National University)
  • Published : 2004.04.01
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Abstract

Choristoneura fumiferana (Cf-2Cl) insect cells were cultured and immobilized by using cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). A concentration of CfMNPV (Choristoneura fumiferana multiple-mucleopolyhedrovirus) and a Cf-2Cl cell density in the microspheres have been achieved at the densities of $1.57\times10^{10}$ PIBs/ml and $7.5\times10^7$ cells/ml, respectively. Additionally, MTT-test was used to measure the viable cell density in the microspheres, and the confidence of MIT-test was investigated before and after baculovirus infection in the immobilized cell culture.

Keywords

References

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