Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Breeding of L(+)-Lactic Acid Producing Strain by Low-Energy Ion Implantation

  • Ge, Chun-Mei (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Gu, Shao-Bin (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Zhou, Xiu-Hong (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Yao, Jian-Ming (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Pan, Ren-Rui (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Yu, Zeng-Liang (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences)
  • Published : 2004.04.01
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Abstract

In order to obtain an industrial strain with higher L(+)-lactic acid yield, the wild type strain Rhizopus oryzae PW352 was mutated by means of Nitrogen ions implantation (l5 Kev, $7.8 \times 10^{14} - 2.08 \times 10^{15} ions/Cm^2$ and two mutants RE3303 and RF9052 were isolated. After 36 h shake-flask cultivation, the concentration of L(+)-lactic acid reached 131-136 g/l, the conversion rate of glucose was as high as 86%-90% and the productivity was 3.61 g/l.h. It was almost a 75% increase in lactic acid production compared with the wild type strain. Maximum fermentation temperature of RF9052 was increased to $45^{\circ}C$ from original $36^{\circ}C$. At the same time, the preferred range of fermentation temperature of RF9052 was broadened compared with PW352.

Keywords

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