Parasites, Hosts and Diseases

  • 제42권2호
  • /
  • Pages.57-60
  • /
  • 2004
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  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지

Enzymatic N-glycan analysis of 31 kDa molecule in plerocercoid of Spirometra mansoni (sparganum) and its antigenicity after chemical oxidation

  • Chung, Young-Bae (Department of Parasitology, College of Medicine and Institute of Medicine, Cheju National University) ;
  • Kong, Yoon (Department of Molecular Parasitology, Sungkyunkwan University, College of Medicine) ;
  • Yang, Hyun-Jong (Department of Parasitology, College of Medicine, Ewha Womans University)
  • 발행 : 2004.06.01
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초록

A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein was subjected to N-glycan enzymatic digestion for structural analysis. The relative electrophoretic mobility was analyzed by SDS-PAGE, before and after digestion. On SDS-PAGE after enzymatic digestion, the 31 kDa protein showed a molecular shift of approximately 2 kDa, which indicated the possession of complex N-linked oligosaccharides (N-glycosidase F sensitive) but not of high-mannose oligosaccharides (endo-beta-N-acetylglucosaminidase H, non-sensitive). Chemically periodated 31 kDa protein showed statistically non-significant changes with human sparganosis sera by enzyme linked immunosorbent assay (ELISA). Therefore, the dominant epitopes of the 31 kDa molecule in human sparganosis were found to be mainly polypeptide, while N-glycans of the antigenic molecule in sparganum was minimal in anti-carbohydrate antibody production.

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참고문헌

  1. Blackwell JM, Ezekowitz RA, Roberts MB, Channon JY, Sim RB, Gordon S (1985) Macrophage complement and lectin-like receptors bind Leishmania in the absence of serum. J Exp Med 162: 324-331 https://doi.org/10.1084/jem.162.1.324
  2. Channon JY, Roberts MB, Blackwell JM (1984) A study of the differential respiratory burst activity elicited by promastigotes and amastigotes of Leishmania donovani in murine resident peritoneal macrophages. Immunology 53: 345-355
  3. Cho SY, Bae JH, Seo BS (1975) Some aspects of human sparganosis in Korea. Korean J Parasitol 13: 60-77 https://doi.org/10.3347/kjp.1975.13.1.60
  4. Cho SY, Chung YB, Kong Y (1992) Component proteins and protease activities in excretory-secretory product of sparganum. Korean J Parasitol 30: 227-230 https://doi.org/10.3347/kjp.1992.30.3.227
  5. Choi SH, Kang SY, Kong Y, Cho SY (1988) Antigenic protein fractions reacting with sera of sparganosis patients. Korean J Parasitol 26: 163-167 https://doi.org/10.3347/kjp.1988.26.3.163
  6. Ellis LA, Reason AJ, Morris HR, et al. (1994) Glycans as targets for monoclonal antibodies that protect rats against Trichinella spiralis. Glycobiology 4: 585-592 https://doi.org/10.1093/glycob/4.5.585
  7. Eylar EH, Jaenloz RW (1962) Periodate oxidation of the $\alpha$1 acid glycoprotein. J Biol Chem 237: 1021-1025
  8. Ferguson MA, Homans SW (1988) Parasite glycoconjugates: towards the exploitation of their structure. Parasite Immunol 10: 465-479 https://doi.org/10.1111/j.1365-3024.1988.tb00236.x
  9. Kim H, Kim SI, Cho SY (1984) Serological diagnosis of human sparganosis by means of micro-ELISA. Korean J Parasitol 22: 222-228 https://doi.org/10.3347/kjp.1984.22.2.222
  10. Kim LS, Kong Y, Kang SY, Cho SY (1992) Immunohistochemical localization of 36 and 29 kDa proteins in sparganum. Korean J Parasitol 30: 25-31 https://doi.org/10.3347/kjp.1992.30.1.25