생명과학회지 (Journal of Life Science)

  • 제14권2호
  • /
  • Pages.320-324
  • /
  • 2004
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Biosurfactant 생산균주 Bacillus sp. TBM 911-5의 분리 및 특성

Isolation and Characteristics of Biosurfactant Producing Bacterium, Bacillus sp. TBM 911-5

  • 김선희 (동아대학교 응용생명공학부) ;
  • 정연주 (동아대학교 응용생명공학부) ;
  • 이상철 (동아대학교 응용생명공학부) ;
  • 유주순 (동아대학교 응용생명공학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 정수열 (동주대학 식품과학계열) ;
  • 최시림 (동아대학교 응용생명공학부) ;
  • 최용락 (동아대학교 응용생명공학부)
  • 발행 : 2004.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 biosurfactant를 생산하는 우수한 균주를 얻고자 산지 토양에서 생산능이 우수한 균주 수십 종을 분리하였다. 분리된 균주 중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적 특성 및 생리 화학적 특성을 조사고, 16S rDNA의 부분적 염기서열 결정을 통하여 Bacillus sp. TBM911-5로 동정하였다. 동정된 균주 배양액에 생산된 biosurfactant에 의해 48시산 정도에서 표면장력이 최저 29mN/m까지 감소되었다. Bacillus sp. TBM 911-5가 생산하는 biosurfactant의 유화활성은 대두유를 기질로 사용하였을 때 최대였으며, 원유에서도 높은 편이였다. 유화안정성은 합성 계면활성제의 Tween 류나 Triton X-100등과 비슷하거나 우수하였으며, 이는 분리된 균이 생산하는 biosurfactant의 계면활성제로서의 산업적 이용 가능성을 보여준다.

The objective of this study was investigate the characteristic of biosurfactant produced from the isolated strain. The strain was isolated from soil samples and identified as Bacillus sp. TBM 911-5 by physiological characteristics and the partial nucleotide sequence analysis of 16S rDNA. We measured the surface tension every 6 hours for 80 hours. The surface tension of the culture filtrate of Bacillus sp. TBM 911-5 was decreased to 29 mN/m. Biosurfactant concentration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The biosurfactant emulsified hydrocarbons, vegetable oil and crude oil. Using soybean oil as substrate, the maximum emulsification activity and stability was obtained from the biosurfactant. The biosurfactant produced from Bacillus sp. TBM 911-5 had strong properties as an emulsifying agent and an emulsion-stabilizing agent.

키워드

참고문헌

  1. Kor. J. Appl. Microbiol. Biotechnol. v.27 Characterization of crude oil degradation by Klebsiella sp. KCL-1 isolated from sea water Cha,J.Y.;B.G.Kim;S.Y.Chung;Y.S.Cho;Y.L.CHoi;Y.C.Lee
  2. Appl. Environ. Microbiol. v.48 Isolation of a bioemulsifier from Candida lipolytica Cirigliano,M.C.;G.M.Carman
  3. Appl. Environ. Microbiol. v.50 Purification and Characterization of Liposan, a bioemulsifier from Candida lipolytica Cirlgliano,M.C.;G.M.Carman
  4. Adv. Appl. Microbiol. v.26 Surface-active compounds from microorganism Cooper,D.G.;J.E.Zajic https://doi.org/10.1016/S0065-2164(08)70335-6
  5. Trends Biotechnol. v.10 Biosurfactant: moving toward industrial application Fiechter,A. https://doi.org/10.1016/0167-7799(92)90215-H
  6. Kor. J. Appl. Microbiol. Biotechnol. v.27 Surface activity and enviromental characteristics of biosurfactant produced by Pseudomonas aerurginosa JRT-4 Hwang,K.A.;J.R.Lee;S.J.Kim;Y.S.Kim;H.J.Ahn
  7. Bergery's manual of systematic bacteriology (9th ed.) John,G.H.;N.R.Krieg;P.H.A.Sneath
  8. Korean J. Biotechnol. Bioeng v.14 Degradation of crude oil and purification of biosurfactant from marine bacterium Pseudomanas sp. CHCS-2 Kim,H.J.;B.J.Kim;S.D.Ha;S.H.Hwang;J.Y.Kong
  9. Korean J. Biotechnol. Bioeng v.24 Degradation of crude oil and purification of biosurfactant from marine bacterium Aeromonas sp. BES-741 Kim,H.J.;B.J.Kim;S.H.Hwang;D.J.Kim;H.W.Lee;J.Y.Kong
  10. J. Ferment. and Bioengin. v.28 Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9 Kim,S.H.;B.D.Yoon;C.H.Lee;H.Y.Seo;H.M.Oh;T.Katsuragi;Y.Tani
  11. Biotech. Appl. Biochem. v.31 Purification and characterization of biosurfactants from Nocardia sp. L-417 Kim,S.H.;E.J.Lim;S.O.Lee;J.D.Lee;T.H.Lee https://doi.org/10.1042/BA19990111
  12. Kor. J. Life Science. v.6 Characteristics of Biosurfactants produced by Bacillus sp. LSC11 Lee,S.C.;Y.J.Jung;J.S.Yoo;Y.S.Cho;I.H.Cha;Y.L.Choi https://doi.org/10.5352/JLS.2002.12.6.745
  13. Biochemical Tests for Identification for Medical Bacteria(2nd ed.) MacFaddin,J.F.
  14. Appl. Environ Microbiol. v.59 Isolation, characterization, and sequences analysis of cryptic plasmid from Acinetobacter calcoaceticus and their use in the construction of Escherichia coli shuttle plasmids Mibas,W.;D.L.Gutnick
  15. J. Biotech. v.12 The influence of phosphate metabolism on biosurfactant production by Pseudomonas aeruginosa Mulligan,C.N.;G.Mahmourides;B.F.Giggs https://doi.org/10.1016/0168-1656(89)90041-2
  16. Appl. Environ. Microbiol. v.48 Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as a sole carbon source Santos,L.G.;O.Kappeli;A.Fiechter
  17. Z. Naturforsch. v.46 Marine biosurfactants, Ⅰ. Screening for biosurfactants among crude oil degrading marine microorganism from the North Sea. Schulz,D.;A.Passeri;M.Schmidt;S.Lang;F.Wagner;V.Wary;W.Gunkel
  18. Soap Cosmet. Chem. Specialities v.70 Surfactants-94 Shaw,A.
  19. J. Microbiol. Biotechnol. v.9 Purification and characterization of biosurfactants produced by Pseudomonas sp. SW 1 Suk,W.S.;H.J.Son;G.Lee;S.J.Lee

피인용 문헌

  1. Characteristics of crude oil biodegradation by biosurfactant-producing bacterium Bacillus subtilis JK-1 vol.56, pp.2, 2013, https://doi.org/10.1007/s13765-012-3269-9