Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Reduction of Oxidative Stress by Chondroitin Sulfate in the Ovariectomy-Induced Aging Rat

난소절제로 유도한 노화쥐에서 chondroitin sulfate에 의한 산화 스트레스의 감소효과

  • 이진영 (인제대학교 의과대학 의학과 생화학전공) ;
  • 하배진 (신라대학교 공과대학 생명공학전공)
  • Published : 2004.04.01
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Abstract

The ovarian hormone-deficiency induced ovariectomy rat is widely used as an aging model due to its practicality, convenience, and cost effectiveness. The surgically ovariectomized rat induces reactive oxygen species (ROS) generation like aging phenomena. Free oxygen radicals have been proposed as important causative agents of aging. The purpose of this study was to investigate the effect of chondroitin sulfate (CS) to prevent ovariectomy (OVX)-induced oxidative stress. The OVX rats were given intraperitoneally CS at doses of 100 mg/kg and 200 mg/kg daily for fifteen weeks. Malondialdehyde (MDA) levels were determined as well as the activities of superoxide dismutase (SOD), catalase (CAT), reduced-glutathione (GSH), oxidized-glutathione (GSSG), glutathione peroxidase (GPx) in the liver. The liver antioxidative enzyme activity was elevated while MDA concentration decreased in all CS treated animals. The results demonstrated that CS reduced oxidative stress in a dose dependent manner. These results suggest that CS might be a useful candidate for antioxidative reagent.

노화과정에 산소라디칼이 관여할 가능성을 난소를 절제한 횐 쥐를 동물모델로 하여 알아보았다. 난소를 절제한 쥐와 난소를 절제하지 않은 정상 쥐에서의 생리활성 및 항산화 효과를 알아보기 위하여 CS를 사용하였다. 난소를 절제한 횐 쥐에 각각 CS를 각 100 mg/kg과 200 mg/kg을 투여하고, 이것과 비교하기 위해 난소를 절제한 대조군과 난소를 절제하지 않은 정상군으로 하여 항산화 효과에 관한 실험방법으로 진행하였다. 노화 유도된 쥐의 각 조직에서 지질 과산화가 증가되었고 유리기 반응이 더 심하게 일어난 간 조직에서 노화 진행이 그만큼 촉진되었음을 알 수 있었다. 산소라디칼 반응이 항진된 원인을 규명코자 간 조직에서 항산화 효소의 활성을 조사한 결과, SOD와 GSH의 활성이 감소되었으며, 노화나 지질과산화가 심하게 진행되었던 간 조직에서 이들 항산화 효소의 활성감소가 심한 경향을 나타내었다. AST의 활성은 OVX 군이 Sham 군에 비해 2.1배 높게 나타났으며, ALT 활성은 크게 변화하지 않았다. 과산화지질한량은 OVX 군이 Sham 군에 비해 1.4배 높게 나타났으며, CS를 투여한 군에서는 31%-38%의 저해율을 보였다. 항산화 효소들은 Sham 군에 비해 OVX 군에서 낮게 나타났으며, 물질 투여 군에서는 정도의 차이는 있지만 대체로 증가하는 경향을 보였다. 이러한 결과에서 보듯이, 난소절제로 생성된 유리기는 물질 투여로 인한 항산화 효소의 증가를 가져오고 이는 결국 유리기로 손상된 막의 지질과산화 정도를 완화시켜 세포를 보호하는 효과를 가져왔다고 할 수 있다. 따라서 CS는 여러 가지 측면에서 난소절제로 인해 유도된 지질과산화 및 활성산소에 의한 공격으로부터 세포를 보호하고 회복시켜주는 효과가 있다고 사료되어진다.

Keywords

References

  1. Methods. Enzymology v.105 Catalase in vitro Aebi,H. https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Toxicol and Appl Pharmacol v.46 Lipid peroxidation and cell damage in isolated Hepatocytes due to bromotrichloromethane Albrecht,D.K.;H.Kappus;H.Remmer https://doi.org/10.1016/0041-008X(78)90095-9
  3. Kor J Gerontol v.7 Effects of aerobic exercise on hepatic antioxidant enzymes in cho-Iesterol-dietary rats Baek,Y.H.;K.W.Kim;T.H.Nam
  4. Anal Biochem v.44 Superoxide dismutase : improved assays and an assay applicable to acrylamide gels Beauchamp,C.;I.Fridovich https://doi.org/10.1016/0003-2697(71)90370-8
  5. Biol Reprod v.45 Functional and morphological changes in the hypothalmo-pituitury-gonadal axis of aged female rats Bestetti,G.E.;M.J.Reymond;F.Blanc;C.E.Boujon;B.Furrer;G.L.Rossi https://doi.org/10.1095/biolreprod45.2.221
  6. Arzneimittelfolschung v.45 Biochemical and pharmakokinetic aspects of oral treatment with chondroitin sulfate Conte,A.;N.Volpi;L.Palmieri;G.Lonca
  7. Arch biochem Biophys v.82 Tissue Sulfhydryl groups Ellman,H.L. https://doi.org/10.1016/0003-9861(59)90090-6
  8. Lab Invest v.47 Biology of disease : Membrane injury and Calcium Homeostasis in the Pathogenesis of Coagu-Iative Necrosis Farber,J.L.
  9. Ann NY Acad Sci v.648 Ovarian steroid and neurotoxin models of brain aging in rodents Finch,C.E.;S.G.Kohama;G.M.Pasinetti https://doi.org/10.1111/j.1749-6632.1992.tb24529.x
  10. J Clin Endocrinol Metab v.77 The relative contributions of menopause and aging to postmenopausal vertebral osteopenia Gambacciani M.;A.Spinetti;L. de Simone;B.Cappagli;S.Maffei;F.Taponeco;P.Fioretti https://doi.org/10.1210/jc.77.5.1148
  11. Aging cell v.1 Oxidative stress and Aging : beyond correlation Golden T.R.;D.A.Hinerfeld;S.Melov https://doi.org/10.1046/j.1474-9728.2002.00015.x
  12. Crit Rev Toxicol v.18 Lipid peroxidation and Mechanisms of Toxicity Horton,A.A.;S.Fayrhurst https://doi.org/10.3109/10408448709089856
  13. J Biol Chem v.261 Difference between N-acetylgalactosamine 4-sulfate 6-O-sulfotransferases from human serum and squid cartilage in specificity toward the erminal and interior portion of chondroitin sulfate Inoue,H.;K.Otsu;S.Suzuki;Y.Nakanishi
  14. Inflamm Res v.46 Ingibition of cartilage degradation in rat collagen-induced arthritis but not adjuvant arthritis by the neutriphil elastase inhibitor MDL Janusz,M.J.;S.L.Durham https://doi.org/10.1007/s000110050233
  15. Cell Immunol v.165 Suppressive effect of a neutrophil elastase inhibitor on the development of collgen-induced arthritis Kakimoto,K.;A.Matsukawa;M.Yashinaga;H.Nakamura https://doi.org/10.1006/cimm.1995.1183
  16. J Reprod Fertil Suppl v.46 Ageing of the neuropeptidergic signals in rats Kalra,S.P.;A.Sahu;P.S.Kalra
  17. Biochemistry v.23 Selective hydrolysis of chondroitin sulfates by hyaluronidase Kundson,W.;M.W.Gundlach;T.M.Schimid;H.E.Conrad https://doi.org/10.1021/bi00297a028
  18. Biochem Biophys Res Commun v.71 Glutathione peroxidase activity in selenium-deficient rat liver Lawrence,R.A.;R.F.Burk https://doi.org/10.1016/0006-291X(76)90747-6
  19. J Biol Chem v.193 Protein Measurement with Folin phenol reagent Lowry,O.H.;N.J.Rosenbrough;A.S.Farr;R.J.Randall
  20. J Mol Biol v.225 X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotrnsferase Mcphalen,C.A.;M.G.Vincent;J.N.Jansonius https://doi.org/10.1016/0022-2836(92)90935-D
  21. Annu Rev Biochem v.52 Glutathione Meister,A.;M.E.Anderson https://doi.org/10.1146/annurev.bi.52.070183.003431
  22. Ann NY Acad Sci v.592 Hormonal influences on reproductive aging in mice Nelson,J.F.;L.S.Felicio https://doi.org/10.1111/j.1749-6632.1990.tb30311.x
  23. Anal Biochem v.95 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Ohkawa,H.;N.Ohishi;K.Yagi https://doi.org/10.1016/0003-2697(79)90738-3
  24. Arzneimittelforschung v.49 Effect of chondroitin sulfate-C on bradykinin-induced proteoglycan depletion in rats Omata,T.;Y.Segawa;Y.Itokazu;N.Inoue;Y.Tanaka
  25. Annu Rev Pharmacol Toxicol v.16 Chemicals, drugs, and lipid peroxidation Plaa,G.L.;H.Witschi https://doi.org/10.1146/annurev.pa.16.040176.001013
  26. J Biol Chem v.217 Glutathione reductase from bakers' yeast and beef liver Racker,E.
  27. Am J Clin Pathol v.28 A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase Reitman,S.;S.Frankel
  28. Experimentia v.50 Effects of chronic caffeine feeding on the activities of oxygen free radical defense enzymes in the growing rat heart and liver Rossowaa,M.J.;T.Nakamoto https://doi.org/10.1007/BF01920748
  29. J Biol Chem v.235 Enzymatic sulfation of mucopolysaccharides in hen oviduct. Ⅲ. Mechanism of sulfation of chondroitin and chondroitin sulfate A. Suzuki,S.;J.L.Stromiger
  30. Nuture v.306 Structure and mechanism of Copper, Zinc superoxide dismutase Tainer,J.A.;E.D.Getzoff;J.S.Richardson;D.C.Richardson https://doi.org/10.1038/306284a0