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Isolation and Identification of Feather-Degrading Bacteria for Biotechnological Applications of Keratinaceous Protein Waste

케라틴 단백질 폐기물의 생물공학적 적용을 위한 우모 분해세균의 분리 및 동정

  • 손홍주 (밀양대학교 생명공학과) ;
  • 김용균 (밀양대학교 생명공학과) ;
  • 박연규 (밀양대학교 환경공학과)
  • Published : 2004.04.01

Abstract

Feathers, which are almost pure keratin protein, are produced in large amounts as a waste by-product at poultry-processing plants. Keratinolytic enzymes may have important uses in biotechnological processes involving keratin-containing wastes from poultry and leather processes. In this study, screening and identification of keratin-degrading bacteria were investigated. Five keratin-degrading bacterial strains (F3-1, F3-4, F7-1, C1-1, C1-2) were isolated from compost and decayed chicken feather. On the basis of morphological, physiological studies, and Biolog system, all isolates were identified as the genus Bacillus. Among them, the strain F7-1 had the highest feather-degrading activity and was selected for further taxonomical study. Phylogenetic analysis of strain F7-1 based on comparison of 165 rDNA sequences revealed that this strain is closely related to Bacillus megaterium.

케라틴으로 구성되어 있는 우모는 도계 공장에서 부산물로 대량 배출되는 단백질 폐기물이다. 현재, 물리화학적 처리를 통하여 우모를 가축의 사료로 재활용하기 위한 방법이 시행되고 있으나 이러한 방법은 많은 단점을 가지고 있으며, 결과적으로 keratinolytic protease의 이용은 우모의 생물 공학적 처리를 위해 반드시 필요한 과정임을 알 수 있다. 본 연구에서는 우모의 생물학적 처리를 위하여 keratinolytic protease를 생성함으로서 우모를 분해할 수 있는 세균을 자연계로부터 분리한 후, 그 분류학적 위치를 검토하였다. 퇴비화중인 볏짚, 닭 가공공장 주변의 토양 및 붕괴중인 우모로부터 keratinolytic protease생성능이 우수한 5균주를 최종 선정하였다. 그중 효소 생성능과 우모 분해능이 가장 우수한 F7-1을 실험 균주로 선정하여 형태학적, 배양적 및 생화학적 특성을 조사한 결과, Bacillus sp.로 동정되었으며, Biolog system을 이용한 동정에서도 Bacillus sp.로 조사되었다. 보다 정확한 균주 동정을 위하여 16S rDNA 염기서열 분석을 수행하였으며, 그 결과 F7-1 균주는 B. megaterium과 계통진화학적으로 가장 유연 관계가 높았다.

Keywords

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  1. Nutritional regulation of keratinolytic activity in Bacillus pumilis vol.30, pp.3, 2008, https://doi.org/10.1007/s10529-007-9567-3
  2. Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1 vol.19, pp.9, 2010, https://doi.org/10.5322/JES.2010.19.9.1077
  3. Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium vol.164, pp.4, 2009, https://doi.org/10.1016/j.micres.2007.02.004
  4. Biological activity of Bacillus spp. evaluated on eggs and larvae of red palm weevil Rhynchophorus ferrugineus vol.65, pp.1, 2015, https://doi.org/10.1007/s13213-014-0881-4
  5. Characterization of Bacillus Species with Keratinase and Cellulase Properties Isolated from Feather Dumping Soil and Cockroach Gut pp.2250-1746, 2018, https://doi.org/10.1007/s40011-018-1026-5