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Chemical Interaction of L-Ascorbic Acid with Ferritin Molecule

가시부영역의 흡수 스펙트럼 변화에 의한 L-Ascorbic Acid와 Ferritin과의 상호작용

  • 김미옥 (대구보건대학 보건다이어트과) ;
  • 나재철 (대구보건대학 보건다이어트) ;
  • 장상문 (대구보건대학 호텔조리음료계열)
  • Published : 2004.03.01

Abstract

This study consisted of designing a sensitive assay to measure the rate of L-ascorbic acid (AsA)-prompted release of ferritin iron, the use of ferrozine as a chelating agent to trap releases Fe(II). The initial rate of iron release was measured in the appearance of Fe(ferrozine)$_3$$^{2+}$ at 562 nm. The release of iron from ferritin by AsA was dependent on time and AsA conditions under aerobic and anaerobic conditions. Effect of oxygen on the release of iron from ferritin was also confirmed. It was suggested that the release of iron from ferritin was participate not only AsA but also $O_2$$^{[-10]}$ . In this study, it was found that iron can be released from ferritin and chelate as Fe(ferrozine)$_3$$^{2+}$ and the release was more than 50% in the presence of AsA without $O_2$$^{[-10]}$ . Based on the findings, the following can be assumed (1) AsA is diffused into ferritin (2) ferric ion is reduced to ferrous ion (3) is diffused from ferritin.tin.

생체 내에 있어서 AsA 대사와 철 대사는 상호 밀접하게 연결되어 있다고 생각하여, 본 연구에서는 철단백질의 하나인 ferritin을 이용해서 AsA와의 상호작용의 유무를 조사하였다. Ferritin으로부터 철의 유리를 측정하기 위하여 ferrozine을 사용하여 AsA에 의한 ferritin으로부터 철의 유리를 흡광도 562 nm에서 Fe(ferrozine)$_3$$^{2+}$의 형태로 측정하는 방법을 사용하였다. 호기적 및 혐기적 조건 하에서 AsA에 의한 영향을 살펴 본 결과, ferritin으로부터 철의 유리에 대해서 AsA의 농도 및 시간의 증가에 의존하는 것을 알 수 있었다. 또한ferritin으로부터 철의 유리에 있어서 산소의 영향을 살펴본 결과, 가시부영역의 흡수 스펙트럼변화의 측정 결과에 의해 호기적 및 혐기적 조건 하 모두에서 ferritin으로부터 철의 유리를 확인하여 산소의 영향이 나타났고, 혐기적 조건하에서 보다 호기적 조건 하에서의 ferritin으로부터 철의 유리가 증가하는 것이 확인되었다. 즉, ferritin으로부터 철을 유리하는데 $O_2$$^{-}$ 가 관여할 가능성이 나타났다. 그러나, 본 연구 결과에서 반응시간 1시간 후의 호기적 및 혐기적 조건 하에서 562 nm에서의 흡광도를 살펴 본 결과 호기적 조건 하에서 유리된 철의 50% 이상이 AsA 자신에 의해, 나머지는 AsA와 산소분자와의 반응에 의해 생긴 $O_2$$^{-}$에 의한다고 생각되어졌다. 지금까지 ferritin으로부터 철의 유리는O$_2$$^{-}$에 의한 것이라는 보고가 많았지만, 이 결과에 의해 ferritin으로부터 철의 유리에 $O_2$$^{-}$가 관여하지만, 그것과 같은 정도 혹은 그 이상 AsA가 중요하다는 것이 밝혀졌다.

Keywords

References

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