Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Dietary Factors on Lymphocyte DNA Damage in Smoking Elderly People in Korea

식이 요인이 SCE 빈도수로 본 흡연노인 임파구 DNA손상에 미치는 영향

  • 강명희 (한남대학교 식품영양학과) ;
  • 이정희 (천안외국어대학 외식산업과)
  • Published : 2004.03.01
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Abstract

The spontaneous frequency of genetic damage and the possible relationship of this damage to dietary and nutritional variables were investigated in peripheral blood lymphocytes from 45 elderly people using sister chromatid exchange (SCE). The relationship of dietary and nutritional factors on SCE was assessed by different degrees of smoking status such as smokers (n=14), ex-smokers (n=16) and non-smokers (n=15). Significant relationship of the SCE frequency to nutrient intake of the combined subjects (n=45) was found. When cigarette smoking status was taken into account, there were negative linear relationships between SCE and fat, phosphorus or vitamin A intakes of the non-smokers as well as SCE and the dietary quality scores. There was a positive linear relationship between SCE and food frequency of meat and fish among the smokers. Use of artificial sweetners in ex-smokers of the elderly people produced a significant increase of SCE in comparison with the mean SCE for those not using sweetners. Other dietary parameters, including intake of coffee, green tea and ginseng tea, alcohol consumption, use of processed foods, and administration of vitamin pills did not show any correlation with SCE. These results suggested that dietary fat, phosphorus or vitamin A status are the major determinants of spontaneous DNA damage in lymphocytes of the elderly people.

영양소의 섭취를 포함한 여러 식이성요인에 따라 일부 지역 남자 노인의 인체 임파구 SCE 빈도수가 어떤 영향을 받는지를 조사해 보고자하여 60세 이상의 노인 45명을 대상으로 설문조사를 실시한 후 채혈하여 임파구 SCE 시험을 실시하였다. 조사 대상자의 식이성 요인이 SCE 빈도수에 미치는 영향에 대해 분석한 결과는 다음과 같다. 1. 채혈 대상자를 흡연여부에 따라 흡연군(n=14), 담배를 피우다가 현재는 끊은 금연군(n=16), 비흡연군(n=15)으로 나눈 후 SCE 빈도수를 비교한 결과 흡연군의 SCE빈도수가 비흡연군에 비해 유의적으로 높았으며 노인의 나이가 많을수록 DNA 손상정도가 증가하였다. 대상 노인의 인구학적 요인 중 신장, 혹은 체중이 전 대상자군에서 SCE 빈도수와 각각 역의 상관관계를 보였으며 흡연군에서는 신장과 SCE빈도수가 역의 상관관계, 그리고 pack years로 본 흡연력과 SCE 빈도수가 정의 상관관계 를 보였다. 2. 전 대상자군에서는 24시간 회상법으로부터 구한 영양소의 섭취와 SCE빈도수 간에 상관관계가 없었으나 흡연군의 경우는 식이섬유질 섭취량, 금연군의 경우는 비타민 C 섭취량과 SCE 빈도수가 각각 정의 상관관계를 보였다. 그러나 비 흡연군에서는 지방, 인 혹은 비타민 A 섭취량과 SCE빈도수 간에 역의 상관관계를 보였다. 3. 흡연군에서 육어류 섭취빈도와 SCE빈도수 간에 정의 상관관계를 보였고, 비 흡연군에서 식 사균형도가 SCE 빈도수와 역의 상관관계를 보였다. 세 군을 모두 합한 전대상자군의 경우, 혈당수준과 SCE 빈도수 사이에 정의 상관관계가 나타났다. 금연군에서 감미료를 섭취하는 군의 SCE 빈도수가 섭취하지 않는 군보다 유의적으로 높았다. 나머지 식이성 요인들은 SCE 빈도수와 상관성을 보이지 않았다. 본 연구 결과는 앞으로 DNA손상도가 높은 노인 대상으로 이의 회복을 위하여 식이성 요인을 이용한 영양중재실험을 계획할 때, 혹은 노인 대상의 영양교육 시에 중요한 기초자료로 활용될 수 있을 것이다.

Keywords

References

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