Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Diacylglycerol Production by Enzymatic Glycerolysis of Soybean Oil.

대두유에서 글리세롤리시스 반응을 이용한 디글리세리드의 효소적 생산

  • 박경준 (서울산업대학교 식품공학과) ;
  • 안은영 (서울산업대학교 식품공학과) ;
  • 권기석 (서울산업대학교 식품공학과) ;
  • 김강성 (용인대학교 식품영양학과) ;
  • 강성태 (서울산업대학교 식품공학과)
  • Published : 2004.03.01
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Abstract

Diglyceride (DG) was prepared by reaction of soybean oil and glycerol in the presence of lipase. The initial rate of DG production was greatly affected by the amount of lipase. However the DG content at equilibrium was hardly affected by the amount of lipase added to the reaction mixture. The initial rate of FFA formation was highly affected by the moisture content between 0.5 and 2.3%, but at higher water content (3.3-5.2%), there was a small increase in the rate. And DG content at equilibrium slowly increased with the increase of the water content in glycerol up to 4.4%. However, there was a sharp decrease in DG content at higher water content (5.2-6.4%) due to higher free fatty acid production. The highest yield of DC was obtained at the temperature ranges of 30-5$0^{\circ}C$. The final yield of DG was not dependent on the glycerol (GL) to triglyceride (TG) molar ratio. However, at the molar ratio of 0.75:1 (GL/TG), the enzyme-catalyzed reaction was highly efficient and utilized all the glycerol. In optimized conditions for glycerolysis a yield of approximately 45% DG was obtained. 66% of total DG was 1,3-DG.

Lipase를 사용하여 대두유와 글리세롤로부터 디글리세리드를 생산하였다. 디글리세러드의 초기 생산 속도는 리파제의 양에 크게 영향을 받았다 그러나 반응혼합물에 첨가된 리파제의 양은 평형상태의 디글리세리드의 함량에는 거의 영향을 미치지 않았다. 초기 지방산 생성속도는 0.5-2.3%의 글기세롤 수분함량에서 매우 영향을 받았으며 3.3-5.2%의 수분함량에서는 느리게 증가하였다 평형상태에서의 DG 함량은 글리세롤 수분함량 4.4%까지 서서히 증가하였으며 5.2-6.4%의 수분함량에서는 지방산함량의 증가로 인하여 오히려 감소하였다 가장 높은 디글리세리드 생산효율을 나타내는 반응온도는 $30-50^{\circ}C$이었으며 글리세롤과 트리글리세드의 몰 비율은 최종적인 디글리세리드 생산율에는 영향을 미치지 많았다. 글리세롤(GL)과 트리글리세리드(TG)의 몰 비율이 0.75:1인 경우에 높은 효소촉매반응을 나타냈으며 첨가된 글리세롤이 반응에 모두 이용될 수 있는 최적조건으로 확인되었다. 최적화된 조건에서 45%의 디글리세리드를 얻을 수 있었으며 전체 디글리세리드 중 65%는 1,3-디글리세리드였다

Keywords

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