대두유에서 글리세롤리시스 반응을 이용한 디글리세리드의 효소적 생산

Diacylglycerol Production by Enzymatic Glycerolysis of Soybean Oil.

  • 박경준 (서울산업대학교 식품공학과) ;
  • 안은영 (서울산업대학교 식품공학과) ;
  • 권기석 (서울산업대학교 식품공학과) ;
  • 김강성 (용인대학교 식품영양학과) ;
  • 강성태 (서울산업대학교 식품공학과)
  • 발행 : 2004.03.01

초록

Lipase를 사용하여 대두유와 글리세롤로부터 디글리세리드를 생산하였다. 디글리세러드의 초기 생산 속도는 리파제의 양에 크게 영향을 받았다 그러나 반응혼합물에 첨가된 리파제의 양은 평형상태의 디글리세리드의 함량에는 거의 영향을 미치지 않았다. 초기 지방산 생성속도는 0.5-2.3%의 글기세롤 수분함량에서 매우 영향을 받았으며 3.3-5.2%의 수분함량에서는 느리게 증가하였다 평형상태에서의 DG 함량은 글리세롤 수분함량 4.4%까지 서서히 증가하였으며 5.2-6.4%의 수분함량에서는 지방산함량의 증가로 인하여 오히려 감소하였다 가장 높은 디글리세리드 생산효율을 나타내는 반응온도는 $30-50^{\circ}C$이었으며 글리세롤과 트리글리세드의 몰 비율은 최종적인 디글리세리드 생산율에는 영향을 미치지 많았다. 글리세롤(GL)과 트리글리세리드(TG)의 몰 비율이 0.75:1인 경우에 높은 효소촉매반응을 나타냈으며 첨가된 글리세롤이 반응에 모두 이용될 수 있는 최적조건으로 확인되었다. 최적화된 조건에서 45%의 디글리세리드를 얻을 수 있었으며 전체 디글리세리드 중 65%는 1,3-디글리세리드였다

Diglyceride (DG) was prepared by reaction of soybean oil and glycerol in the presence of lipase. The initial rate of DG production was greatly affected by the amount of lipase. However the DG content at equilibrium was hardly affected by the amount of lipase added to the reaction mixture. The initial rate of FFA formation was highly affected by the moisture content between 0.5 and 2.3%, but at higher water content (3.3-5.2%), there was a small increase in the rate. And DG content at equilibrium slowly increased with the increase of the water content in glycerol up to 4.4%. However, there was a sharp decrease in DG content at higher water content (5.2-6.4%) due to higher free fatty acid production. The highest yield of DC was obtained at the temperature ranges of 30-5$0^{\circ}C$. The final yield of DG was not dependent on the glycerol (GL) to triglyceride (TG) molar ratio. However, at the molar ratio of 0.75:1 (GL/TG), the enzyme-catalyzed reaction was highly efficient and utilized all the glycerol. In optimized conditions for glycerolysis a yield of approximately 45% DG was obtained. 66% of total DG was 1,3-DG.

키워드

참고문헌

  1. Yeast v.14 Candida rugosa lipases. Molecular Biology and Versatility in Biotechnology Benjamin,S.;A.Pandey
  2. J. Org. Chem. v.64 A New approach to the stereospecific synthesis of phospholipid use of L-glyceric acid for the preparation of diacylglycerol Farzaneh,S.;D.W.Roodsari;S.P.Gregory;H.Joseph
  3. Biochim. Biophys. Acta. v.912 Lipase-catalyzed ester synthesis in oil-continuous microemulsions Flecher,P.D.I.;R.Freedman;B.Robinson;G.Rees;R.Schomacker
  4. Appl. Biochem. and Biotechnol v.82 Lipase-catalyzed production of short-chain acids trepenyl esters of interest to the food industry Francoise,L.;P.robert
  5. J. Am. Oil Chem. Soc. v.66 Enzymatic glycerolysis of a triglyceride in aqueous and nonaqueous microemulsions Holmberg,K.;b.Lasesen;M.Strak
  6. J. Am. Oil Chem. Soc. v.65 Enzymatic preparation of monoglycerides in microemulsion Holmberg,K.;e.Ostberg
  7. J. Am. Oil Chem. Soc. v.61 Continuous synthesis of glycerides by lipase in a microporous membrane reactor Hoq,M.M.;T.Yamane;s.Shimizu
  8. J. Ferment. Bioeng v.80 Esterification of glycosides by a mono-and diacylglycerol lipase from Penicillium camembertii a comparison of the products with Candida cylindracea lopase Hirofumi,N.;S.Kitabata;S.Yuji;N.Masaki;T.Yoshio
  9. Biotechnol. Lett v.20 Rapid enzymatic production of acylglycerols from conjugated linoleic acid and glycerol in a solvent-free system Jose,A.;C.O.Arcos;G.C.Hill Jr.
  10. Kor. J. Food Sci. Technol. v.30 Development and use of carbohydrate polyesters Jung,H.Y.
  11. Kor. J. Food Sci. Technol. v.26 Effect of temperature on diacylglycerol production by enzymatic solid-phase glycerolysis of hydrogenated beef tallw Kang,S.T.;T.Yamane
  12. J. Lipid Res. v.2 Separation of lipid classes by chromatography on florisil Karroll,K.
  13. EP0377154, A3, B1, B2 Kao corp(JP) Preparation of diglycerides Kohori,J.;Y.Kawakara;Y.Hirota
  14. J. Am. Chem. Soc. v.53 Food emulsifiers: Surface activity, edibility, manufacture, composition, and application Lauridsen,J.B.
  15. Wiedrmann: Nautrition and technic of edible oil Lee,Y.J.;H.Lars
  16. J. Am. Oil Chem. Soc. v.67 Solid phase enzymatic glycerolysis of beef tallow resultion in a high yield of monoglyceride McNeill,G.P.;S.Shimazu;T.Yamane
  17. J. Am. Oil Chem. Soc. v.68 High yield enzymatic glycerolysis of fats and oils McNeill,G.P.;S.Shimazu;T.Yamane
  18. Biochim. Biophys. Acta v.912 Lipase-catalysed ester synthesis in oil-continuous microemulsions Paul,D.I.;f.Robert;b.Freedman;H.Robinson
  19. Kor. J. Food Sci. Technol v.30 Producion of unique microbial lipid Sin,Y.C.
  20. Bailey's Industrial Oil and Fat Products v.2 In Fat splitting, esterification, and interesterification Sonntag,N.O.N.
  21. J. Am. Chem. Soc. v.59 Glycerolysis of fats and methyl esters Sonntag,N.O.V.
  22. Appl. Biochem. Biotechnol. v.50 Production of glycerides from glycerol and fatty acids by native lipase of Nigella sativa seed Sunna,M.L.Dandik;H.A.Aksoy
  23. LIPIDS v.18 Quantitative determination of Tri-, Di-, Monooleins and Free Oleic Acid by the Thin Layer Chromatography-Flame Ionization Detector System Using Internal Standards and Boric Acid Impregnated Chromarod Tatara,T.;T.Fujii;T.Kawase;M.Minagawa
  24. JAOCS v.71 High-yield diacylglycerol formation by solid-phase enzymatic glycerolysis fo hydrogenated beef tallow Yamane,T.;S.T.Kang;K.Kawahara;Y.Koizumi
  25. J. Jpn. Oil. Chem. Soc. v.35 Glycerolysis of fat by lipase Yamane,T.;M.M.Hoq;S.Ithoh;S.Shimizu
  26. J. Jpn. Oil Chem. Soc. v.35 Continuousglycerolysis of fat by lipase in microporous hydrophobic membrane reactor Yamane,T.;M.M.Hoq;S.Ithoh;S.Shimizu
  27. Kor. J. Food Sci. Technol v.30 Production and use of functional lipids Yun,S.H.;K.S.Kim;S.W.Zoe
  28. Development of enzymatic reactor for production of emulsifier and useful materials Korea Advanced Institute of Science & Technology
  29. Determination of lipolytic activity of lipase for industrial use v.601 Japan Industry Standard