Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Thermostable Xylanase-producing Paenibacillus sp. DG-22.

내열성 Xylanase를 생산하는 Paenibacillus sp. DG-22 균주의 분리 및 효소 특성

  • Published : 2004.03.01
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Abstract

A new moderate thermophilic bacterial strain DG-22 which produces thermostable xylanase was isolated from a timber yard soil in Kyungju, Korea. On the basis of morphological, biochemical and phylogenetic studies the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. No cellulase activity was detected. The temperature and pH for optimum activity were 8$0^{\circ}C$ and 5.0-5.5, respectively. The crude xylanase was stable at $60^{\circ}C$ and retained 60% of initial activity after 2h at $70^{\circ}C$. Zymogram analysis of the culture supernatant showed two xylanase active bands with molecular masses of 22 and 30 kDa.

경주지역에 있는 목재 저장소의 토양으로부터 내별성 xylanase를 생산하는 호열성 세균인 DG-22 균주가 분리되었다. 형태적, 생화학적, 계통학적 연구에 근거하여 이 분리균은 Paenibacillus 속하는 종으로 판명되었다. 이 균주에서 xylanase의 생산은 성장배지에 xylan을 첨가함으로서 유도되었고 glucose또는 xylose에 의해서 억제되었다. Cellulase 활성은 탐지되지 많았다. 효소 활성을 위한 최적온도와 pH는 각각 $80^{\circ}C$와 5.0-5.5이었다. 이 조효소는 $60^{\circ}C$매서 안정하였고 $70^{\circ}C$에서는 2시간 추에도 60%의 활성을 유지하였다. 배양상등액의 zymogram 분석을 통해 22 kDa과 30 kDa 크기의 xylanase활성 band를 확인하였다

Keywords

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