Journal of Korea Technical Association of The Pulp and Paper Industry (펄프종이기술)

Korea Technical Association of the Pulp and Paper Industry (한국펄프종이공학회)
권호 표지

Recycling of Waste Paper with Alkaline Cellulolytic Enzyme (II) - Purification of alkaline cellulolytic enzymes and characteristics of reaction with fiber -

호알칼리성 목질분해 효소를 이용한 폐지 재생(제2보) - 알칼리성 목질분해 효소 정제 및 섬유 반응 특성 -

  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Alkaline cellulolytic enzymes from cultured medium of Coprinus cinereus 2249 were purified with gel and ion-exchange chromatography and characteristics of those enzyme proteins were investigated. A fiber length distribution and a crystallinity of cellulose and sugar composition of enzyme treated Mixed Office Wastepaper(MOW) and Unbleached Kraft Pulp(UKP) were analysed. The conclusion could summarized as follows; \circled1 Alkaline and acidic, endo- and exo-glucanases were purified from cultured medium of Coprinus cinereus 2249. \circled2 The approximate molecular weight of alkaline endo-glucanase was 42 kDa, and also that of alkaline exo-glucanase was 50 kDa. A fiber length distribution and a crystallization of cellulose and sugar composition of enzyme treated MOW and UKP were not so much changed with original paper and pulp.

Keywords

References

  1. Appl Microbiol Biotechnol v.53 A comparison of enzyme aided bleaching of softwood paper pulp using combination of xylanase, mannase and galactosidase Clarke,J.H.;K.Davidson;J.E.Rixon;J.R.Halstead;M.P.Fransen;H.J.Gilbert;G.P.Hazlewood
  2. Enzyme and microb. Technol. v.3 Biodegradation of cellulosic materials: Substrate, microoganisms, enzyme and products Bisaria,V.S.;Ghose,T.K.
  3. American Chemical Society Toner removeal by alkaline active cellulase from desert Bisdiomycetes, ACS Symposium series 655 Sreenath,H.K.;Yang,V.W.;Burdsall,H.H. Jr.;Jeffries,T.W.
  4. Appita v.48 no.5 Separation by flotation of contaminants from recycled fiber Nishi,K.;Pratima,B.;Pramod,K.B.
  5. Tappi Journal v.77 no.4 Comparienzyme enhance with conventional deinking of xerographic and laser printed paper. Jeffries,T.W.;Klungness,J.H.;Sykes,M.S.;Rutledge-cropsey,K.R.
  6. Tappi Journal v.82 no.5 Enhancing douglas-fir pulp properties with a combination of enzyme treatments and fiber fractionation Mansfield;Shawn D.;Shawn,D.;Jason;Roberts;Norm;Olson;James A.;Saddler;John N.
  7. Tappi Journal v.76 no.3 Enzymic modification of secondary fibers Jackson,L.S.;Heitmann,J.A.;Joyce,T.W.
  8. Pulp and paper v.9 Enzymatic deinking of offset waste Morgan,B.R.
  9. Tappi pulping conf. Alkaline cellulases and enzymatic deinking of mixed office waste Abukakr,M.;Rutledge-Copsey,K.;Klungness,J.H.
  10. Korea TAPPI v.35 no.3 Recycling of waste paper with alkaline cellulolytic enzyme(1) Kang,S-H.;J-Mj.Lee;S-B Park;T-J.Eom
  11. Nucl. Instr. and Meth. in Phys. Res. A. v.380 The effect of charge traping on the spectrometric performance of n-type cardnium detectors Iwanczyk,J.S.;Debrowski,A.J.