Korean Journal of Environmental Biology (환경생물)

  • Volume 22
  • /
  • Pages.38-46
  • /
  • 2004
  • /
  • 1226-9999(pISSN)
  • /
  • 2287-7851(eISSN)
Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Effects of Polycyclic Aromatic Hydrocarbons Contamination on the Community Structure of Microorganisms in Gwangyang bay Sediments

다환방향족 탄화수소가 광양만 퇴적토의 미생물 군집구조에 미치는 영향

  • 권개경 (한국해양연구원 해양미생물연구실) ;
  • 정성영 ;
  • 이정현 (한국해양연구원 해양미생물연구실) ;
  • 현정호 (한국해양연구원 해양미생물연구실) ;
  • 김상진 (한국해양연구원 해양미생물연구실)
  • Published : 2004.03.01
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Abstract

Effect of polycyclic aromatic hydrocarbons (PAHs) on the community structure of indigenous microorganisms in Gwangyang Bay sediments was investigated in Mar. & Aug.,2000. Microbial community structure was analyzed using 5'-terminal restriction fragment length polymorphism (T-RFLP) method. Microbial community structure based on T-RFLP method revealed that community differentiated by sampling period except station 1 located near the stream discharge site from Yeosu Industrial Complex. Even, microbial diversity was higher at stations showed relatively high concentrations of PAHs. The microbial community structure was severely changed during the enrichment culture with 1,000 ppm of PAHs mixture. It was also different between cultivated at 8$^{\circ}C$ and 30$^{\circ}C$. The results implied that temperature, poyosity, organic content and etc were more responsible than PAHs on the microbial community structure.

다환 방향족 탄화수소(PAHs)에 의한 오염이 미생물군집구조에 미치는 영향을 파악하기 위하여 연안에 가까운 광양만 퇴적토를 대상으로 하여 2000년 3월과 8월 2회에 걸쳐 PAHs 오염도와 말단제한절편 다형성 (T-RFLP)분석방법을 이용하여 미생물 군집구조를 조사하였다. T-RFLP방법으로 조사한 미생물 군집은 계절에 따라 군집이 구분되었으며 월내천입구에 위치한 정점 1에서 3월에 다른 정점과 다른 독특한 군집구조를 형성하였다. 또한 상대적으로 PAHs 오염도가 높은 정점들에서 미생물 다양성도 높은 것으로 나타났다. PAHs 농후배양시료에서의 미생물군집구조 변화와 비교해 볼 때 조사대상지역 퇴적토의 미생물 군집구조는 PAHs의 오염에 부분적으로는 영향을 받지만 군집구조를 결정하는 주된 요인은 온도, 입도, 유기물 함량 등과 같은 환경요인인 것으로 사료된다.

Keywords

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