생명과학회지 (Journal of Life Science)

  • 제14권1호
  • /
  • Pages.174-179
  • /
  • 2004
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Hydroxyapatite 인산염 가용화 균 Klebsiella sp. DA 71-1의 분리와 가용화특성

Isolation and Characteristic of the Phosphate Solubilizing Bacteria Klebsiella sp. DA 71-1

  • 이진우 (동아대학교 응용생명공학부) ;
  • 정연주 (동아대학교 응용생명공학부) ;
  • 이경아 (동아대학교 응용생명공학부) ;
  • 최시림 (동아대학교 응용생명공학부) ;
  • 김영길 (동아대학교 식품과학부) ;
  • 최용락 (동아대학교 응용생명공학부)
  • 발행 : 2004.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

난용성 인산염을 가용화시키는 균주를 염류직접 및 인산과다처리 재배지로부터 다수 분리하였다. 다수의 균들은 대다수가 tri-calcium phosphate의 가용화능을 가진 균주들이 었고 hydroxyapacte의 가용화능을 가진 균주가 소수있었다. 본 연구는 hydroxyapatite 가용화능을 가진 분리균주 Klebsiella sp. DA7l-1의 배양특성에 따른 난용성 인산염(hydroxyapatite, tri-calcium phosphate, aluminium phosphate), pH변화 및 배양온도에 따른 인산염의 가용화특성 등을 조사하였다 인산 가용화능은 유기산 생성과 밀접한 관련이 있다고 많이 보고되어 졌지만 아직 pH저하에 따른 인산 가용화의 정확한 기작은 확인이 되지않은 상태이며 배지내의 pH저하는 균주의 유기산 생성에 따른 것으로 사료되어진다. 조사한 결과 배양시간 12 이후부터 pH 저하를 나타냈으며 pH저하는 균생육에 영향을 미치지 않는 범위내에서 이루어졌으며 pH가 저하된 상태의 배지 조건에서 균의 생육이 계속될수록 인산가용화능이 높게 이루어졌다. 또한 DA7l-1은 초기 pH에 영향을 크게 받지 않았으며 일반 토양의 온도와 비슷한 조건의 온도에서도 가용화 능력이 뛰어난 것으로 나타났다.

To develop high effiency biofertilizer, a bacterium having ability to solubilize inorganic phosphate was isolated from cultivated soils, using a sucrose minimal agar-hydroxyapatite medium. The strain was identified as Klebsiella sp. DA7l-1, based on the physiological and biochemical properties. The activity of solubilizing inorganic phosphate of Klebsiella sp. DA7l-1 against three types of insoluble phosphate such as tri-calcium phosphate, aluminium phosphate, hydroxyapatite were quantitatively determined. The results indicated that the strain solubilized hydroxyapatite. The MPS (mineral phosphate solubilizing) conditions of Klebsiella sp. DA7l-1, were measured to determine the optimal conditions. The optimal temperature and initial pH to solubilize insoluble phosphate in sucrose minimal medium were $30^{\circ}C$ and pH 6.0, respectively.

키워드

참고문헌

  1. Lett. Appl. Microbiol. v.18 Phosphates and the unilization of organic phosphorus by Rhizobium leguminsarum Abd-Alla, M. H. https://doi.org/10.1111/j.1472-765X.1994.tb00873.x
  2. Groundnut News. v.6 Response of groundnut to phosphate solubilizing microorganisms Agasimani,C.;A.Mudlagiriyappa;M.N.Sreenivasa
  3. Introduction to soil microbiology Alexander,M.
  4. Crop. Res. Hisar. v.5 Phophate solubilizing microorganism(PSM) as inoculant their role in augmenting crop productivity India-A review. Dubey,S.K.;S.D.Billore
  5. Am. J. Alt. Agric. v.1 Bacterial solubilization of mineral phosphates : historical perpectives and fufure propects. Goldstein,A.G.
  6. World J. Microbiol. & Biotechnol. v.14 Effect of buffering on the phophate-solubilizing ability of microorganisms. Gyaneshwar,P.;Naresh,K.G.;Parekh,L.J. https://doi.org/10.1023/A:1008852718733
  7. Soil. Biol. Biochem. v.27 Solubilization of hardly-soluble AIPO₄with P-solubilizing microorganisms. Illmer,P.;A.Barbato;F.Schinner https://doi.org/10.1016/0038-0717(94)00205-F
  8. Soil. Biol. Biochem. v.27 Solubilization of inorganic calcium phosphate-solubilization mechanisms Illmer,P.;F.Schinner https://doi.org/10.1016/0038-0717(94)00190-C
  9. Soil Biology & Biochemistry. v.27 Solubilization of inorganic Phosphates by microorganisms isolated from forest soils. Illmer,P.;F.Schinner https://doi.org/10.1016/0038-0717(94)00190-C
  10. Korean J. Biotechnol. Bioeng v.13 Isolation and cultural characteristics of a phosphate-solubilization fubgus, Penicillium sp. PS-113 Kang.S.C.;M.C.Choi
  11. Cheju National University Journal v.17 Mycorrhizae distribution and rock phosphate dissolution by soil fungi in the citrus fields in Jeju-do Kim.H.O.;Z.K.Uo;S.C.Lee;R.M.N.Kucey
  12. Microbiology v.31 Bacterial ferilizers, their effectivencess and mode of action Mishustin.E.N.;A.N.Naumova
  13. Soil Microbiology and Biochemistry Paul.E.A.;F.E.Clark
  14. Soil. Biol. Biochem. v.13 Influence of soil inoculation with vesicular-arbuscular mycorrhiza and a phosphate-dissolving bacterium on plant growth and $^{32}$P uptake Raj,J.;D.J.Bagyaraj;A.Manjunath https://doi.org/10.1016/0038-0717(81)90004-3
  15. Mycological Res. v.99 Solubilication of insoluble metal compounds by soil fungi:Development of a screening method for solubilizing ability and metal tolerance. Sayer,J.A.;S.L.Raggtt;G.M.Gadd https://doi.org/10.1016/S0953-7562(09)80762-4
  16. Lehrbuch der Bodenkunde Scheffer,F.;P.Schachtschabel
  17. Soil. Biol. Biochem. v.8 Utilization of rock phospate in alkaline solis by plants inoculated with mycorrgizal fungi and phosphate-solubilizing bacteria Azcon,R.;Bares,J.M.;Hayman,D.S. https://doi.org/10.1016/0038-0717(76)90078-X
  18. J. Korean Soc. Agric. Chem. Biotechnol. v.44 Isolation and Cultural Charateristics of a Phosphate-Solubilizing Bacterium, Aeromonas hydrophila DA57. Song,O.R.;S.J.Lee;S.H.Kim;S.Y.Chung;I.H.Cha;Y.L.Choi
  19. Ind. J. Agr. Res. v.27 Rock phosphate-superphosphate in wheat in relation to inoculation with phosphate solucilizing organism and organic waste Tiwari,V.N.;A.N.Pathak;L.K.Lehri
  20. Ind. J. Exp. Biol. v.31 Solubilization of natural rock phosphates and pure insoluble inorganic phosphates by Aspergillus awamori Varsha,N.;T.Jugnu;H.H.Patel

피인용 문헌

  1. Antifungal Activity of Bacillus sp. GJ-1 Against Phytophthora capsici vol.41, pp.2, 2013, https://doi.org/10.4489/KJM.2013.41.2.112
  2. Mechanisms of Phosphate Solubilization by PSB (Phosphate-solubilizing Bacteria) in Soil vol.45, pp.2, 2012, https://doi.org/10.7745/KJSSF.2012.45.2.169