생명과학회지 (Journal of Life Science)

  • 제14권1호
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  • Pages.98-103
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  • 2004
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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논물의 미생물군으로부터 해조분해능을 갖는 균주의 분리

Isolation of a Seaweed Hydrolytic Strain from the Microflora in Water of a Paddy Field

  • 김해섭 (여수대학교 식품공영양학부) ;
  • 최옥수 (순천제일대학 식생활학부) ;
  • 강동수 (여수대학교 식품공영양학부) ;
  • 박욱민 (여수대학교 식품공영양학부) ;
  • 백승한 (순천제일대학 식생활학부) ;
  • 배태진 (여수대학교 식품공영양학부)
  • 발행 : 2004.02.01
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초록

해조 또는 해조성분을 가수분해시키는 효소를 생산하는 미생물이 존재할 것으로 추정되는 논물에서 채취한 미생물군 시료에서 다시마분말 첨가 고체배지를 이용하여 순수배양을 실시하여 16균주를 분리하였으며, 이들 균주를 다시마분말 첨가 액체배지에서 3주간 배양하며, 1주 간격으로 전당과 환원당의 함량을 측정하여 추출율을 비교한 결과 배양 전 기간에서 30A412균주가 높은 추출율을 나타내었다. 30A412 균주를 제외한 나머지 15균주는 3주간의 배양으로도 그 추출율이 10%이하를 나타내었다. 30A412균주의 알긴산, 미역 및 다시마 분말 첨가 배지에서의 성장율은 배양 1주일 후부터 서서히 증가하였으며, 대조구보다 높은 성장율을 보였다. 알긴산, 다시마 및 미역 분해율은 배양기간이 경과함에 따라 증가하는 경향을 보였다. 균체 성장율과 분해율 모두 다시마 배지에서 가장 높은 값을 나타내었으며, 다음으로 미역배지, 알긴산 배지의 순이었다.

Various bacterial strains were isolated from water in a paddy field, and their seaweed degrading activities were investigated. As the result, 16 strains were obtained from the microflora sample. They were incubated in a liquid medium of sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of the liquid medium were measured once a week during the incubation period. Ratio of reduced sugar to total sugar of 30A412 strain was highest. Accordingly, 30A412 strain was incubated in three different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week during the incubation period. Ratios of reduced sugar to total sugar and cell growth were increased with the incubation period. Cell growth and degrading ratio were highest in the liquid medium with sea mustard powder.

키워드

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