Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Ultrastructure of Cell Wall in the Suppressor Mutant of Null Pigmentation (SU-NPG) of Aspergillus nidulans

Aspergillus nidulans 색소결핍 억제돌연변이주의 세포벽 미세구조

  • Published : 2004.02.01
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Abstract

To investigate the effect of the cell wall on the pigmentation and branching in Aspergillus nidulans, ultrastructure of cell wall in suppressor mutant of the null pigmentation (SU-NPG, SU602) has been examined. Scanning electron microgrphs (SEM) revealed that the most outer layer of conidia wall peeled off in SU-NPG on day 6 from the complete conidiation. They also showed that hyphal growth and branching were not well developed in SU-NPG. Transmission electron micrographs (TEM) showed that the plasma membrane was not crenulated and the hyphal wall was thick in SU-NPG. These results indicated that the ultrastructure of cell wall in SU-NPC might be modified. Cytochemical analysis showed that the cell wall in SU-NPG was differentiated into Cl, C3, C2 and C4 layer in conidia and H1, H3, H2 and H4 layer in hyphae. C3 layer and H3 layer existed in SU-NPG. The increment of the diameter in SU-NPG hyphae might be caused by the thickness of H3 layer. These results suggest that SU-NPG may have an immature but the differentiated structure for the pigmentation in cell wall.

A. nidulans의 색소결핍 억제돌연변이주(Suppressor mutant of the null pigmentation; SU-NPG)의 세포벽 구조가 색소형성 및 균사분지에 미치는 영향을 조사하여 다음의 결과를 얻었다. SEM으로 세포 표면구조를 관찰한 결과, 완전배지에서 배양된 SU-NPG의 분생포자벽은 최외각 층이 6일 째부터 박리되었으며, 균사생장이 저조하였다 TEM으로 관찰한 결과, 균사 세포벽은 매우 두꺼워졌다. 이러한 전자현미경관찰 결과는 이들 돌연변이주의 세포벽 구조가 NPG 및 야생형의 그것과는 다르다는 것을 시사한다. 균사에 탄수화물 염색을 한 후, 세포화학적 미세구조를 관찰한 결과, 분생포자벽은 C1, C2, C3와 C4 층으로 되어 있었고, 균사 세포벽은 H1, H2, H3와 H4층으로 되어 있었다. 이러한 C3층과 H3층은 세포분화 시 색소형성 및 균사 분지형성에 필수적인 세포벽 구조임 을 시사한다. SU-NPG는 FGSC4에서와는 다르게 비정상적인 균사생장을 하였지만, 색소결핍 돌연변이를 억제하는데, 이는 SU-NPG의 균사 세포벽에는 H3층이 형성되었으며, 세포벽 형성과정은 정상적으로 진행되기 때문으로 사료된다.

Keywords

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