Journal of Plant Biotechnology

  • 제31권1호
  • /
  • Pages.61-65
  • /
  • 2004
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Alocasia cadieri Chantrier의 기내번식

In Vitro Propagation of Alocasia cadieri Chantrier

  • 한봉희 (원예 연구소 원예생명공학과) ;
  • 예병우 (농촌진흥청 연구관리과) ;
  • 구대회 (원예연구소 화훼과) ;
  • 유희주 (원예 연구소 원예생명공학과)
  • Han, Bong-Hee (Horticulture Biotechnology Division, Horticulture Research Institute) ;
  • Yae, Byeoung-Woo (Research Coordination Division, Rural development Administration) ;
  • Goo, Dae-Hoe (Floriculture Research Division, Horticulture Research Institute) ;
  • Yu, Hee-Ju (Horticulture Biotechnology Division, Horticulture Research Institute)
  • 발행 : 2004.03.01
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초록

본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia의 경정을 TDZ 0.1mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5mg/L첨가된 배지에서 효과적이었다. 형성된 신초의 발근은 IBA또는 NAA가 첨가된 배지보다는 활성탄 2.0g/L가 첨가된 배지에서 양호하였으며, 소식물체의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.

In order to micropropagate uniform plantlets of Alocasia cadieri Chantrier in vitro, the shoot tips were cultured on media containing various concentrations of BA and thidiazuron (TDZ). Multiple shoot formation from shoot tips was very effective on medium containing 0.1mg/L TDZ. The formed shoots from shoot tips were separated into a shoot, and cultured on media with BA, TDZ, and NM combination for proliferation. The shoots were multiplied very vigorously on medium with 0.5mg/L TDZ and 0.5mg/L NAA. The rooting and growth of multiplied shoots were more effective on medium with 2.0g/L activated charcoal, rather than those with IBA and NAA. Rooted plantlets show high survival in soil mixed with perlite 1: vermiculite 1 or vermiculite alone.

키워드

참고문헌

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