이차원 전기영동에 의한 단백질의 분리

Analysis of Protein by Two-dimensional Gel Electrophoresis

  • Woo Sun-Hee (Department of Agronomy, College of Agriculture, Life and Environment, Chungbuk National University) ;
  • Kim Sun-Lim (National Institute of Crop Science, RDA) ;
  • Jong Seung-Keun (Department of Agronomy, College of Agriculture, Life and Environment, Chungbuk National University) ;
  • Kim Hong-Sig (Department of Agronomy, College of Agriculture, Life and Environment, Chungbuk National University)
  • 발행 : 2004.10.01

초록

키워드

참고문헌

  1. Cleveland, D. W., Fischer, S. G., Kirschner,. M. W, and Laemmli, D. K (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J. BioI. Chern. 252: 1102-1106
  2. Fukuda, M., Islam, N., Woo, S. H., Yamagishi, A., Takaoka, M., and Hirano, H. (2003) Assessing MALDI/TOF as a means of rapid embryo protein identification in rice. Electrophoresis 24 : 13191329 https://doi.org/10.1002/elps.200390168
  3. Hirano, H. (1982) Varietal differences of leaf protein profiles in mulberry. Phytochemistry 21 : 15311518
  4. Hirano, H. and Watanabe, T. (1990) Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: Application to an insoluble protein. Electrophoresis 11 : 573-580 https://doi.org/10.1002/elps.1150110708
  5. Hirano, H. (1997) Screening of rice genes from the cDNA catalog using the data obtained by protein sequencing. J. Protein Chemistry 16: 533-536 https://doi.org/10.1023/A:1026377931210
  6. Ichimura, H., Yamamoto, K., Havukkala, I., Ohta, I., Mukai, Y, Hirose, S., Song, J., Nagasaki, H., Shinozuka, Y, Hamamatsu, C., Itadani, H., Kojima, S., Yamanouchi, U., Hamada, M., Ohkubo, N., and Nakamura, Y (1995) Rice cDNA classification into non-redundant groups. Rice Genome 4 (2) : 1
  7. Islam, N., Woo, S. H., Tsujumoto, H., Kawasaki, H., and Hirano, H. (2002) Proteome approaches to characterize seed storage proteins related to ditelocentric chromosomes in common wheat (Triticum aestivum L.). Proteomics 2(9) : 1146-1155 https://doi.org/10.1002/1615-9861(200209)2:9<1146::AID-PROT1146>3.0.CO;2-6
  8. Ito, K., Kimura, Y, Sassa, H., and Hirano, H. (2000) Identification of the rice 20S proteasorne subunits and anlaysis of their N-terminal modification. Jpn, J. Electroph. 44: 205-210
  9. Komatsu, S., Kajiwara, H., and Hirano, H. (1993) A rice protein library: A data-file of rice proteins separated by two-dimensional electrophoresis. Theor. Appl. Genet. 86: 935-942
  10. O'Farrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chern. 250: 4007-4021
  11. Pearson, W. R. and Lipman, D. J. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acda. Sci. USA. 85 : 2444-2448 https://doi.org/10.1073/pnas.85.8.2444
  12. Sasaki, T., Song, Y, Koga-Ban, E., Matsui, F., Fang, H., Higo, H., Nagasaki, M., Miya, E., Murayama-Kayano, T., Takiguchi, A., Takasuga, M., Niki, K., Ishimaru, H., Ikeda, Y, Yamamoto, Y, Mukaim, I., Ohta, N., Miyadera, I., Havukkala, Y, and Minobe, Y (1994) Toward cataloguingall rice genes: large-scale sequencing of randomly chosen rice cDNA from a callus cDNA library. Plant 1. 6: 615-624. https://doi.org/10.1046/j.1365-313X.1994.6040615.x
  13. Tsugita, A., Kawasaki, A., Uchiyama, Y, Kamo, M., Miyatake, N., and Nozu, Y (1994) Separation and characterization of rice proteins. Electrophoresis 15: 708-720 https://doi.org/10.1002/elps.1150150198
  14. Woo, S. H., Fukuda, M., Islam, N., Takaoka, M., Kawasaki, H., and Hirano, H. (2002) Efficient peptide mapping and its application to identify embryo proteins in rice proteome analysis. Electrophoresis 23 : 647-654 https://doi.org/10.1002/1522-2683(200202)23:4<647::AID-ELPS647>3.0.CO;2-O
  15. Woo, S. H., Higa, A., Kimura, M., long, S. K., and Yamaguchi, I. (2003) Proteome analysis of wheat lemma. Biosci. Biotechnol. Biochem. 67: 2486-2491 https://doi.org/10.1271/bbb.67.2486
  16. Woo, S. H., Kim, H. S., Song, B. H., Lee, C. W., Park, Y M., Jong, S. K., and Cho, Y. G. (2003). Rice proteomics : A functional analysis of the rice genome and applications. J. Plant Biotechnology. 30:281-291 https://doi.org/10.5010/JPB.2003.30.3.281