Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Mass Production of HzSNPV Baculoviruses in Immobilized Heliothis zea (HzAM1) Insect Cell Culture

  • Son Jeong Hwa (Department of Biotechnology & Bioengineering, Pukyong National University) ;
  • Buchholz Rainer (Department of Bioprocess Engineering, Friedrich-Alexander University) ;
  • Kim Sung-Koo (Department of Biotechnology & Bioengineering, Pukyong National University)
  • Published : 2004.10.01
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Abstract

Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMl cell density was $7.5{\times}10^7$ cells/mL in the microspheres. After infection of the immobilized cells by Heliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was $2.87{\times}10^{10}$ PIBs/mL in the microspheres.

Keywords

References

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